FastQCFastQC Report
Sat 31 Dec 2022
SRR5849666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65287
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT4920.7535956622298468No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC4690.7183665967191017No Hit
TAGAGAGGGAGTACATAAATGCCTTTTTACAGCTTAATCATTTTGGTCTTCT1140.17461362905325717No Hit
GCATCTGGGGTGGGGTTTTTTCCTGTATGGGAAACCATTTATGCCAAGCTTT1010.15470154854718396No Hit
ATAAGCGGGATGTGGTTTTCAAAAAATTGTTGAAGACGTGACTTCATAGCAA990.15163815154624963No Hit
TCTCAGGGGCCTGTGAACACTGAAAGAGAAAGCCTAATGTAAAGTAGTGATG980.15010645304578246No Hit
ACTAAAGGGGAGGTTCAGTGGATGATTTTTGTTCTGTCACTGATTTTTTCAC980.15010645304578246No Hit
GAGTCCGGGGTGGTCCTTTTACTCAAAATCGTTCTATATCTTGCCTGTCTGC970.1485747545453153No Hit
TGCAAATGGGATCTAGTTAACATTTTAATTTTAAAATTGCCAACTTTTGGGA960.14704305604484813No Hit
TATAGAGGGAAGATCACCTTTGTGGATTTCCTTGCCTATGATGTCCTTGACA960.14704305604484813No Hit
TATGGTGGGGGAATTACGCTCGCAGAGCTGCGGGCTCTCATGGAGCTCAGGT950.14551135754438096No Hit
GACAATGGGTCATTTGTAGAGCTTCGAATTCCTTGCCCCTGAAAATAAGCTC930.14244796054344663No Hit
TCCTAAGGGATAGCCACATACATCTAGATTAGACAAGAAGAAATATCTCAAG920.14091626204297947No Hit
TCATTTGGGGATTGTATTGAGGATTAGTCTCAGTTGGAAAGCCAACTACAGA910.1393845635425123No Hit
GCTTAGGGGTCGCCAGAGGTGCTTCACCCTGAGTTTTGTTTTGTATTGTTTT910.1393845635425123No Hit
CTCGATGCGGGGAGTATCTGCTTGTTAAAATGGAAAAATGTGTCTTTAGAGC900.1378528650420451No Hit
ACAGCAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT890.13632116654157797No Hit
GGTTTAGGGAGACAAGTAAATAAATATAAGACAAAAATCATTCATAATCTTA890.13632116654157797No Hit
CGCTGTGGGCGATCTGATATAGGGACTTGTTTCTGAAATAAAATTCTGAAGG880.1347894680411108No Hit
GAACACGGGATTTGAAGAATGTCAGCAGAATTTAATGAAACTCCAGAAAGGA880.1347894680411108No Hit
AGCACTGGGAGGTTCCAACATAGGAATTTTGGGGGGACACCAACATTTGGAT860.13172607104017645No Hit
TTCAGTGGGTGTCCTTGTCAATTTTTTTTACTGATAAAGCAGGATAAGTCTG860.13172607104017645No Hit
TCACAAGGGATACATGGATGATGATGTAATTGTGCAAGGTGATATTCTTGCC860.13172607104017645No Hit
TCCGTGGGGAGTGCAGAGAATGTGGAAAATCCTTTATGTACAACTACCGACT850.13019437253970928No Hit
GACTTTAGGGTCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAA850.13019437253970928No Hit
CTAACAGGATTGAATAAAATTTTATATAGGCAGTCCCCAGCCCTTTCCTCCT840.12866267403924211No Hit
CTTGCTGGGAATTCTTGCATGTTTGTATCTAATTTCTGAACGAATGAGCACA840.12866267403924211No Hit
AAAACAGGGATATGTGTTCTTGTTCTGATGTTGTTTCCTGAAATGATTTTTC830.12713097553877495No Hit
TCAGCAGGGGACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCTGTCGAGCAGC830.12713097553877495No Hit
ATGAATGGGAGGTTTACATTACTGATTTCCAGAAGTAAGTAGTTTCCCCTTT820.12559927703830776No Hit
GGCTTAGGGGAAGCATTATTTTCCCAACACAAAAGAGCTTTTTTCTCGGTAT820.12559927703830776No Hit
CTCACGGGCTTATTTTACTTAATAATGACCCCAAAGTGCAAGAGTACTGATG820.12559927703830776No Hit
AAGTGCGGGGGGAGTCTGCAAAGGAGCCTGGCCTCCCCCATTGCCAGAACTG820.12559927703830776No Hit
TATCAAGGGGATCTGAATTGGGGATCATCCACACGGGGCAGTCGGCCCTCGG820.12559927703830776No Hit
CTTATAGGGGATAACACATAATCTCCAACAGAAGTTACTGAATACATTCATA820.12559927703830776No Hit
GTTTCAGGGAACATGGTGTGAGGACGGACTGGGAGCCGGTACAGACTCCAGT820.12559927703830776No Hit
TCGTACGGGGAAACATTTTCTAGGAAAAATAAAGGCAAACATACAATGTCAA810.1240675785378406No Hit
TCATGCGGGATTCGATATCTCAGCTCTAGAATAGCCCTGTGCTAAGAATGAC810.1240675785378406No Hit
TTCTTCGGGTACACAATTCTGACTCTGCTCAGTGGGTGCCAAAACTGTTGCT810.1240675785378406No Hit
GCTGAAGGGATCAACTCAGTAGCCCACACCCAGTTATCTGGGAGCTGATGGT810.1240675785378406No Hit
GATCATGGGCAGCCAGAACGCCTGAACGCAGGCACATACTTCCTATTCTACA800.12253588003737345No Hit
ATTAAAGGGAGCCCTGATGATCTCTGGAATCATCTTTGGGGTCATTCTTCCA800.12253588003737345No Hit
TATGTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA800.12253588003737345No Hit
AATCTGGGACCTGACAAGTCCTAACCATGATTATTAAATCTAAGAGAGACTG800.12253588003737345No Hit
GGAATCGGGAAAGCGAAACTATTTTTATAGAGAAAAAGGGTCTTTCTTAACG790.12100418153690627No Hit
TGGCCAGGGACTATAATTTACTTTTGACTTAATGGGGCATAAATAAAACTTT790.12100418153690627No Hit
TAGCTTGGGGACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTG790.12100418153690627No Hit
CAGCAAGGGGCCTGTAATCCCTGCTGCTTGGGAGGCTGAAACAGGAGAATCG780.1194724830364391No Hit
GCTGCTGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC780.1194724830364391No Hit
TTATTTGGGACAGTAGGAAACGTCCCAATAAGGTAACTTTTTCAGAATTGAA780.1194724830364391No Hit
AACAAAGGGATAGCACTCAATACGAAATATTCTAGGGATATTTTACTGTAGT780.1194724830364391No Hit
CGTGACGGGCATCCTTTGAATTCACTCGGAGGCAAGGTGGTCATTTCTTATC780.1194724830364391No Hit
CTACTAGGGGGGCCAACCTTGACTACCCCGGCCCAGGGCTCACCTACAAGAC780.1194724830364391No Hit
CGTTTTGGGTAGGTGTGAGCACTGCACCCAGCCTTGTGTTTTTTTTCTACAT780.1194724830364391No Hit
TGAACTGGGGGCACACAGCGAATAGGCTGGTTCTCCATGAGGATTGTATTTT780.1194724830364391No Hit
CAAGTTGGGGTGCTAAGTTAAAATACAGTTTAGTTATTTGCTTTAAAATAAA780.1194724830364391No Hit
CTTTAGGGGGAAGTCAATCATTTTTCCTATAGGGCTCTGTTTCCTTTTCTTT780.1194724830364391No Hit
GCTAGGGGGTTCTGCATCCACCGATTCAAACAACTGTGGAACAAAATTGTCA780.1194724830364391No Hit
TACCATGGGGTACACTTGGTGTAACTGTATGGAAATATAGTTTCTAGCTTTT780.1194724830364391No Hit
GGTCTAGGGGACATAGCAATTCTACTCCTGTATATGTGCCCAAGAAAGATGA770.11794078453597194No Hit
GTATTCGGGAAGACTCTTCTCCAAAATTTTGATAAATGGCTTTTTATAATAA770.11794078453597194No Hit
TACCATGGGGAAGAAAAAAAACCTCATATAATTTCAGCTTTTTGGAAGAAAG770.11794078453597194No Hit
TAACGAGGGAACAGTTAAGAAGCAAAGGCAATTATTAAAGATTTGGTGACTC770.11794078453597194No Hit
GTTATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC770.11794078453597194No Hit
GAATGATGGGAGTTCAGTGCTGACCATTAAAAACTATCATGCTTGATACGGT770.11794078453597194No Hit
AATCCTGGGATCCCTTGTCCTCTGACATTAAATTATGTAGAGAAAGGCTTCC760.11640908603550476No Hit
TTAAGTGGGGCTGCACTGCATCAGTATCTTACTAAAAATGTGAAATGAAAGG760.11640908603550476No Hit
TGTGGCGGGTATTGCTACGGAAACTGAGAACCAAGGGGCAGAAAGATAACCT760.11640908603550476No Hit
TTGCCAGGAAGTCCTTGTTCCTGGTGAACCACAGTTAGGGTGTTTTGTTTAT760.11640908603550476No Hit
GTTATCGGGGACATAATGAAATAATGAAATGAGATGAAATAAAATAATGAAA760.11640908603550476No Hit
ACTTATGGGTGCTATTTCTTTCTAGAATTTATTTGCCATTTTACCTGTTTGG760.11640908603550476No Hit
CTGCTAGGGATCAACAAAGAGATAATATATTTTAAAAAGAAGTAAACAAATT750.1148773875350376No Hit
AAGAGTGGGGATGCTTGACTTAAAGCTCTACAGCTAGCAGTAATAATCATAT750.1148773875350376No Hit
TTCGCAGGGAGTCGCGAGCGAACGACCAAGAGGGTGTTCGACTGCTAGAGCC750.1148773875350376No Hit
GTGCTGGGAATGTGGCTAATAGTGGTATGATTTCTAGCCCGTGGGTTTAAAA750.1148773875350376No Hit
CCACTGGGGATTGTAGCTTATATAACATTTTCTAAATTTAGATATCCCATCA750.1148773875350376No Hit
TTCATAGGGAAGTAGATCAAACTAGCTTTTAAATGGATTAGCTTGTTCTCAA750.1148773875350376No Hit
TGTCATGGGCTACCTGCGGGATACTAACGTGTTTATTGAGAGCATTTTACCT740.11334568903457044No Hit
AAAATGGGATGAGACTAGGGCTATGATTATAAATGATTAGGGAAAATGATTA740.11334568903457044No Hit
CGTTATGGGCTGGTGAACATTTTTCCAATTACATGGATATGAAACATTTTGT730.11181399053410326No Hit
ATAGATGGGGGGAAACAAATCTGTCCAAAAAGAAAGTCTGTGAGAAGCTAAG730.11181399053410326No Hit
CACGGTGGGTCCAATCAAAGTTTTAAAAACACCATTTTAAGTTATGTTCAGT730.11181399053410326No Hit
AATACAGGGAAATTTTTAAGTCTGTAAATATATTTTAAAGTTATATAATAAT730.11181399053410326No Hit
GGTCTAGGGATTCAGGTTTATGAAGGCGAGCGTGCCATGACAAAGGATAACA730.11181399053410326No Hit
CTATCAGGGATATCCAGAGTTCATGCTTCTGGATGAAGACCGTGGCTTTTGG730.11181399053410326No Hit
TTTGGCGGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTAT720.1102822920336361No Hit
CTTCATGGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAACGAGGTG720.1102822920336361No Hit
TTCATGGGGAAAGAGACTGAGAGAAATTAATAGTATGATACGCACAGGAGAA720.1102822920336361No Hit
TGATGCGGGAGTTCAAGATTTTCGGTATATAAAATCTGTCCTTTCCTACCTG720.1102822920336361No Hit
ACGTGAGGGATGTGAACACATTGACTATTAAGCAAACTTAAGAGTGGTTGGA720.1102822920336361No Hit
TTCTAGGGGGAAGGTGTAGTTCAAGTAATTACTACAAATAATTAAAGCCCTA710.10875059353316893No Hit
CTACGCGGGATTGACTTCTAATTGTGAATTAGTTTCCCTTTTCTACTTGTTT710.10875059353316893No Hit
GTATGGGGGATACCTTCCTTAGAGAAATGTCAATTCATATAACTTGCACGTT710.10875059353316893No Hit
ACCAAAGGGATTAAACTATTGGATAGAGGAAAATGGTCCAAGATAAGAACAT700.10721889503270175No Hit
CTTCTTGGGGAGGAAGTAATGTATGGCCTTCCACGTGGATTAATTTCACTCT700.10721889503270175No Hit
GGATTAGGGAAGTCAAATCCTTATCAGATCATTGTTGTATTCTAAGGAGTGA700.10721889503270175No Hit
CACATGGGGAATGTTGATGACTGGATTCCTATTTATATAGTATTTGGGGGCT700.10721889503270175No Hit
AGACCCGGGGACTCACAGTTCTGCAGAGCTGGGGAGGCCTCAGAAAACTTAC700.10721889503270175No Hit
GTCACAGGGCTATCGGGGGGGAAAATGAAATGGAAATGATGAGATGAGAAGA700.10721889503270175No Hit
TAATGTGGGAGCATAAATTATTTGCACCTTAATAAGTCCTCAGTTTGAACTA700.10721889503270175No Hit
GCTACTGGGATGATGATCATTGTGAAAATTCTGAGTTGAAGGTTAGTTTAAG700.10721889503270175No Hit
TAAACAGGGTGGTTATGATATCTTCACAGATAACACAGGCAAATTATCATTA700.10721889503270175No Hit
TGCTTGGGGAGACTGAAAGTGAAGGAATGGACAAAGATATCCCATGCAAATT690.1056871965322346No Hit
TATAGCGGGATTCTTATTTCTGATTGAGTATTGAATTTACATTTTTCAGAAT690.1056871965322346No Hit
TGTCGGGGAGCCGTTTTCCTTCAGGAACATAGATGCAAATTTCCTCAAAAAT690.1056871965322346No Hit
ACTTCTGGGGAAGCATCTAGATTAGACATCTGAGGGCCGTTTAACCTTCTGC680.10415549803176744No Hit
ATCTGTGGGGAGAGTCCTTGCCAGTTTTGTAACAAAGAGACCAACAATCATT680.10415549803176744No Hit
CATTAAGGGCAGGGCATGCCGGTCATCCTGAGACCTTCCTTTAACCTCCTTT680.10415549803176744No Hit
ACAGCCGGGATCTTTTTTGTTTTTGAGACAGAGTTTCGCTCTTGTTGCCTAA680.10415549803176744No Hit
AGCCTGGGCTTGATGGGGGATGCACTTCTGACAACTATCTGCTAAGAAAACT670.10262379953130026No Hit
CTGAAAGGGGAACCTGGGAGGCGGAGCTTGCAGTGAGCTGAGATCGCGCCAC670.10262379953130026No Hit
TCTGTGGGGCTTCAAAACTAAGGAGTTGCAGAAAAAAATGGATTTCACAGAG670.10262379953130026No Hit
GAATTCGGGGGTTGGGTGGGGCGGAGCGTCTGGGATGCGCTGGGAGCCTAGG670.10262379953130026No Hit
ACACGTGGGACTCATAGTTTATAAAATGGCAGAAAATGTATACAAACTAATT670.10262379953130026No Hit
GCTATAGGGCCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG670.10262379953130026No Hit
GAATTAGGGGACAAAGGAATCTTTATTAGTCAGGATTAAAGTAGTCCATGAT670.10262379953130026No Hit
AGCAGGGGGATAGATCATCTTGAAGTATTATTTCACATTGAAAAGAAGAAAA660.10109210103083309No Hit
ATAACAGGGAGCCTTATAAAAGGGAAGGTAAATGGCATAAATATGGTCGCAC660.10109210103083309No Hit
TGCGCCGGAATAATAAAGGGTTTTATTTTCCTTCATATTTTTGTTTTTAGCA660.10109210103083309No Hit
TTTAGAGGGTGTCTTACAATTGAATTCATTTTGCTGTTGAATGGGAAAGCAG660.10109210103083309No Hit
TGTAGCGGGCCGATCTGATAGAGGGCAGTAATCATACAGTTTTGCACTCAAT660.10109210103083309No Hit
TTACATGGGACATGAAATGATGACATAAAATGAGATGAAATGAGATGTAATG660.10109210103083309No Hit
TTAATACTGGGATTGACCAATAGGTTAGGTTTCCCAAAATAGTTTCATTTAG660.10109210103083309No Hit
ATTGTTGGGCAATTAATGCTGAACATGAAGAGTAAAGTATTTATCTGAAAGA660.10109210103083309No Hit
CTGAGTGGGAAACCCGCCAGCCCTGCGTTCTGTTCTAGGAGCTGCAGATGGT660.10109210103083309No Hit
GCACTTGGGAGGGTTCTACGTGATGGCTAATGGAATGTTTAGATCAGTTCAC660.10109210103083309No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)