FastQCFastQC Report
Sat 31 Dec 2022
SRR5849665.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849665.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100759
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT6380.6331940571065612No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC5160.512113061860479No Hit
AACTAAGGGGCTCTCACTTTAATTTCATCCTGTCATTAGTAGACAAACGCTT1610.1597872150378626No Hit
CACAACGGGATATCAAGCAGGGATGGCAAACTGAAGAGCTGAGGAAATGATG1560.15482487916712154No Hit
AGTAGTGGGAGAATTTTGTATCCAGCAAATCTAAGTTTCGTAAATGAAGAAA1530.1518474776446769No Hit
CACTAGGGGAAGCTCATGTTTTTAAAGATCGTTTATTGAGATGATTTTGAAA1450.14390774025149117No Hit
TTGTTAGGGATCCTACTTTTAACAAGCTGTTCTGAAGCTTAATCCAGTTACA1430.14192280590319475No Hit
GCTCGGGGACTTCTCTGAATGTCCAGTTGGAAAGATGTAGAGTTGTTGCCCA1420.14093033872904653No Hit
GCGTGAGGGGCACAGGAAGCTTTCTGCATATTCTTTCAAGAGATTTCGAGGT1410.13993787155489834No Hit
TGAAGGGGGAGATCTAAAATCTTCACTACTATGTAACCGTAGGTAGGTTGTC1400.1389454043807501No Hit
GTCAGCGGCGCAGCATGCACCAAGGTTATTCGTTATTTACTAGTTCATTCAC1390.13795293720660187No Hit
GTCAAAGGGGTAGTTTGCTTTTGATTTCAAAATGCCACCTCTTCTGATTCCA1390.13795293720660187No Hit
CATACAGGGGTGCGTGTCAACCCCACCGACTGCTGCAAACAGTGTCCAGTGG1380.13696047003245368No Hit
AAGGCCGGGATTTGCCAAAAGGAGGCACTTTTTATTTAAAATTTGAGACTAA1380.13696047003245368No Hit
GTCGCGGGAGAGCAGCAATCTTTGAAATTAGTCAACAATGCAGTCTTTTTTA1370.13596800285830546No Hit
TTTGCTGGGGGGAGTGTAAATTGGTTCAGCCATTGTGGAGAGCAGTGGGGTG1340.13299060133586083No Hit
AATACTGGGATATTGTCTCTTGTAAAATCCTTACTCTCTCTTTCTCTGCCCA1330.1319981341617126No Hit
TGAATAGGGACACCAACCAGCTTTTCACTTTCGTGGTTTCGTCACGGTTCTG1310.13001319981341616No Hit
GCACCTGGGCTTCTGATGGGAAATCTTATGATAATGCATGCCAAATCAAAGA1290.12802826546511975No Hit
TGAGCTGGGGAGTTTTCCTAACCTATTGATACTGCTTATACATGTAAGCAAG1270.1260433311168233No Hit
TACGCGGGGAGGCTTTCCCCTTTCTTTCTGTTCTAGCAGCAGGAAAAGTTTC1270.1260433311168233No Hit
TACACAGGGGTCATGAAAAGTCCAGTGGAGAAAATCTCAATGCTTACTGTTA1270.1260433311168233No Hit
GAAATGGGGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTG1250.1240583967685269No Hit
CGAGTAGGGAGGATTAAGAAACTCAGTCAAAACTGCACAACTACATGGAAAC1250.1240583967685269No Hit
ACAAGGAGGGACAAGCTGATGGGAGAGAAGAATAGAGCAGATTGCAGTAGGA1250.1240583967685269No Hit
GAATGCGGGTCGCCGGGATATGGGAAAAATTGAATTTTGGAAACAAGGACCA1250.1240583967685269No Hit
TTTTCAGGGCAAACACATACCCATACATATTTTAAGATATGCTGTTAATCAG1250.1240583967685269No Hit
TAGCTAGGGGCCTCAGGTGGTTCATCCCTTTTGGCACTCATGGTGCCACTTG1240.12306592959437866No Hit
TTAGGGGGAGGCATATTCTTTTCTCTTGCTATCCCCTCTACCTGAAATGATC1230.12207346242023044No Hit
GGGGACGGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTA1230.12207346242023044No Hit
AATACTGGGTGGCTGAATCATCCGCTACCTTCACGCCAATGGCGCCTCAATA1230.12207346242023044No Hit
AGAGAAGGGACAAATACATTGTCTTAACAATGAAGTCTATGTACATTTGATT1220.12108099524608225No Hit
CAGCCAGGGCGTGGTCTGTGCAAACACTTCGTGGTTCTATATATCAGCAGCA1220.12108099524608225No Hit
CGAAGGGGGAGATACGTGATTGTTAATGATTTATTTTCCTTTCCTTTTCTCT1220.12108099524608225No Hit
ATCTTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1200.1190960608977858No Hit
GCTACAGGGGTATTAAGCTATTTGCTTCTTTCTCTCTCTCTTTTTTTTTTTT1200.1190960608977858No Hit
AGCTAAGGGAAGAAGTGTTACCTTATTAAAATGACCACCATTCTAAACCATT1200.1190960608977858No Hit
ATAAGGGATAGTGTTTTAGTGATGACTGAGAAAATAGGTTGAGTTTTTTTAA1190.1181035937236376No Hit
TACACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1190.1181035937236376No Hit
CGGTCCGGGGAAGTAGATGCTGATGGTAATGGCACAATTGACTTCCCTGAAT1180.11711112654948937No Hit
TAAGGCGGGTGTTTAGAGTTCTGTTGTCAAAAGAAAACTAATGAATAAATTA1180.11711112654948937No Hit
TCGTTAGGGATGTATTGCAAACCAGAGATCTCAGTCAGATCCCCTAAGGGGA1180.11711112654948937No Hit
GAAATAGGGATTGGAAATCTCAGATGAAATACATTTCTAGTAGTACCACCAG1180.11711112654948937No Hit
ACACAAAGGGAGAAAAGGAAGGAAATAATAAAGATAAAAATATAAATAAGTG1170.11611865937534117No Hit
AGCGAAGGGGAGCATTAAAAATCATCAGCATTTAACTGAGACCCCACTATAG1170.11611865937534117No Hit
TATTTCGGGGGTGGGGATGTACCTTAAGTTTCCTTGTGGAATAATAGACTTT1170.11611865937534117No Hit
TCATTGGGAGCACCCCTAGTCATGAAAATAAAATGTCTAGACATTGCCAAAC1170.11611865937534117No Hit
TGTATAGGGATCCCTGATACAAACTGTTGCTAATTACCATCTTTATGGCCTG1170.11611865937534117No Hit
GTATTGGGGAATAAATATAAATGAGATAAATAAAAGAATACATATTTTACCC1160.11512619220119295No Hit
CACAGCGGCTGGCGCTGGGATCTGTAATGGTGGAGAAAATGTTTTGTTACTT1160.11512619220119295No Hit
ATCAACGGGGATCTTTAGAAGGGGGTTGAGGTAGAGGGAGCTACAAAGATTA1160.11512619220119295No Hit
TACCTGGGGAAAGGCATCGAGCCTTCCGCATCGCTGATCCAGTGGTTCTGGG1150.11413372502704472No Hit
AGTAGGGAGTTCTGAGTTTTGACAAGCATATACAGTCATGTAACCACCACAA1150.11413372502704472No Hit
TCAGGCGGGATGCCAAGCCAGACTGACTACAGCCTCCTCTGAACTCTGTTGT1150.11413372502704472No Hit
TCAGCTGGGTGTTTGAAAGCCACTGGATCCGATTTGGAAGCAATATCATCAT1150.11413372502704472No Hit
GATCTGGGGAAAGCAATATTATTTCCAAGTATATTAATTTGTTAGAAAACTT1140.11314125785289653No Hit
AGCTACGGGAAAGTAAATTAGTATTGCTGCAAATAGCTAAATTATATTTATT1140.11314125785289653No Hit
ATCGTGGGGAGCCGCGGGATACATATATGGATGTATTTATAATGACATATAA1140.11314125785289653No Hit
AATTTTGGGGACGGCTCTTCCGATCTTAATCAGGGCATTCAGGCACTTGGTC1140.11314125785289653No Hit
CCAAGAGGGGCTCCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGT1130.1121487906787483No Hit
GTTTGCGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTG1130.1121487906787483No Hit
TACTAAGGGTGAAATGGGAACAAGCAGCCAATTTTCACCTTAAATTTTACTT1130.1121487906787483No Hit
CAGTCTGGGGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTA1130.1121487906787483No Hit
AGAAGCGGGGTGTCTCCATGAGAATCAAATACAGAAACGTGAGCAAATGACG1120.11115632350460007No Hit
TGCCATGGGATGCCTACATCAAGAACTTAGAATGATCACAAATTTACAACCT1120.11115632350460007No Hit
GGGTCTGGGACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAAT1120.11115632350460007No Hit
CGTATGGGTAGAGCAGAGATCATAACCAAGGAGAGAGAGGTCTCAGAATGGA1120.11115632350460007No Hit
TGCTAAGGGGAGGACATCCACCAGTACAGGGAGCAGATCAAACGGGTGAAGG1120.11115632350460007No Hit
CCTACCAGTTGTATTGTCTCCAACTAGTTCACCTTTCTGAGCCTCCAGGGCC1120.11115632350460007No Hit
ACTATGGGGACTGCTTTGAATTGGATGAATGAATTTGAGAAGTGGCAAGAGG1120.11115632350460007No Hit
AACACTGGGCCCTTAAGAACCTGACCCCAGTGAATGAAGCTGATGCACAGGG1110.11016385633045188No Hit
CCCCACTGCAGATCCTCCAGATGACTGACCTACTCTAAAGCCCACTGCTCAG1110.11016385633045188No Hit
CTACACGGGATTGCACTTTACATAAGACAGGCAAACCACAGGTTCTTGTGGA1100.10917138915630366No Hit
TACCGTGGGATCAGTCAAGATCCCACACAAGAAACACAACCAATAGGATACT1090.10817892198215545No Hit
TGAACAGGGAGTACTGAATTTATCCATAAACTCGAAGCTCTGCTGCAAAGAG1080.10718645480800723No Hit
TGTCCAGGGAGGCTATCTTTCAGTGGCCATTACTATGTTTCGTACACATGCT1080.10718645480800723No Hit
ACGTATGGGAGGGTTAATTTTAAATAATGATGCAAATCCCAAACCTGATCTA1080.10718645480800723No Hit
GCATAAGGGCGATCTAAATACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC1070.10619398763385901No Hit
AAGCTCGGGATATACAGACAAATGATTGCATGAGGCCATGTTTATATGTGTG1070.10619398763385901No Hit
ATGTCAGGGACCAACTTGGATTAACATACTGGTTCATGGGTGAAGAGACAAG1070.10619398763385901No Hit
TTAGAGAGGGACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCA1060.1052015204597108No Hit
ATTTATGGGTGACTTACCTGTTCAGTACTCTACAATTCCTCTGATAATATAT1060.1052015204597108No Hit
CTAACAGGGATATCTTCTGATGTGGAGATTTGAGAAGCATTTGTATTGGATG1060.1052015204597108No Hit
TGTAGTAGGTATTGTGACTTCATAATTATTTGGAATAAGATTGGTGATGTTG1060.1052015204597108No Hit
CTTACTGGGCTTGCAGTGGTATCTTTTCTTTGGGTTACAGTGTTGTTCATCC1050.10420905328556258No Hit
AGACATGGGGCTACAGAGCAGGAGTTGCCGCAGCCCCAGGCTGAGACAGGGT1050.10420905328556258No Hit
TCACAGGGGGAAATAAAAAAATTTAAAAATTTGACTTTATTGGAAAAGTACT1050.10420905328556258No Hit
CGACGGGGGAAAAGATTTAAGTAATTTTGCCATCCTAGGCTTAAATGCAATA1050.10420905328556258No Hit
GCATCTGGGCTTCCACAGGGATCTAGGAGTGTGGAGATTGTAAAATTCCCCA1050.10420905328556258No Hit
GCCTGTGGGTCCAAGCCATAGCTTGGGCTGAAGGACAAATTGAAATTGTCTG1050.10420905328556258No Hit
GATCCAGGGCATATGAGCAGTATGTCTGATGTGTAAAAAGATAATTCATTTG1050.10420905328556258No Hit
GCTGTTGGGATGGTTTTATAACTCTCTCATTCATATTCAGACACAATGCAAA1040.10321658611141436No Hit
AGAGGCGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC1040.10321658611141436No Hit
TGTGGTGGGACTACTCATTAGCGCACTGGCTAAGCTGCCTTTGCCTTCTCGT1040.10321658611141436No Hit
TGAGGCGGGATAGTAGCTATACAAAATATATCTCTAGGCCCTAAAAATTTGC1030.10222411893726616No Hit
TTCTTAGGGTCTTTTTGTAGACATTTAAATACTGTTTATACTCCAACCTGGA1030.10222411893726616No Hit
GATTAAGGGTGGTTGCTGATGTTAGATTATTGTTAAACTGTAATTACTAATT1030.10222411893726616No Hit
CGGAATGGGACCAAGACGTCTGCTTTTGTGACAGTCAGAAAACACACCTGGA1020.10123165176311794No Hit
TTCTCAGGGAGAGACTAGAATTGTGGAGGTTGGGACCTTGTAACTCCAATAA1020.10123165176311794No Hit
ATAATGGGGCTAGCTGGGGGAACTGGACTGGTGGGGGCTCCAGCATGTGGTG1020.10123165176311794No Hit
TCTTTAGGGATCCAAGTCTACCAAGGTAACTAAGTAATGGATGTTTTCTCTC1020.10123165176311794No Hit
ACAAGGGAGGCAGAAGACCAGAGAATCTGAACCCTTGAGCAAATCTAAACAT1020.10123165176311794No Hit
GTGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.10023918458896972No Hit
GATTCTGGGAGGATATAACTTATCAGCAACCAAGACTTACAGCATGGTATGG1010.10023918458896972No Hit
CAATAGGGGATTCTCTATTTTTTAATGTTACTCAGATTTGATATGCTATGCT1010.10023918458896972No Hit
GCTGGGGGATTTTTCAGACTGGCCATTGAAGTTATATACCTAAGGATAGTGC1010.10023918458896972No Hit
TAGCTCGGGAAGTGCTGAAAAGGAAGGTGTCTAGGAGTGACACTGACGTGTT1010.10023918458896972No Hit
AATCGAGGGAGCATTCAATTATGCCAGTTCCATTAGAATACATGAAAGAACT1010.10023918458896972No Hit
CAATAGGGGATGCTGAATCAATGTTTCTCAGCCCTTAAATGGCCTTTCTGTG1010.10023918458896972No Hit
ATGTGGGGACATTCCAGAGTCTAAGCATGGAATTTAAGTTTTGTATTTTGAA1010.10023918458896972No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)