FastQCFastQC Report
Sat 31 Dec 2022
SRR5849664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100305
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT6740.671950550819999No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC5280.5263944967848063No Hit
GCACCTGGGCTTCTGATGGGAAATCTTATGATAATGCATGCCAAATCAAAGA1450.14455909476097903No Hit
CACAACGGGATATCAAGCAGGGATGGCAAACTGAAGAGCTGAGGAAATGATG1430.14256517621255171No Hit
ACAAGGAGGGACAAGCTGATGGGAGAGAAGAATAGAGCAGATTGCAGTAGGA1420.14156821693833807No Hit
GAAATAGGGATTGGAAATCTCAGATGAAATACATTTCTAGTAGTACCACCAG1410.14057125766412443No Hit
GTCAGCGGCGCAGCATGCACCAAGGTTATTCGTTATTTACTAGTTCATTCAC1390.13857733911569714No Hit
ACTAAAGGGACATGAAAGTATGGGCACCAAAACGTTAAAAGTGGTTTTGTTG1380.13758037984148347No Hit
GTCAAAGGGGTAGTTTGCTTTTGATTTCAAAATGCCACCTCTTCTGATTCCA1380.13758037984148347No Hit
GCTCGGGGACTTCTCTGAATGTCCAGTTGGAAAGATGTAGAGTTGTTGCCCA1380.13758037984148347No Hit
AATACTGGGATATTGTCTCTTGTAAAATCCTTACTCTCTCTTTCTCTGCCCA1360.13558646129305618No Hit
TACACAGGGGTCATGAAAAGTCCAGTGGAGAAAATCTCAATGCTTACTGTTA1360.13558646129305618No Hit
CGAGTAGGGAGGATTAAGAAACTCAGTCAAAACTGCACAACTACATGGAAAC1350.13458950201884254No Hit
AGCTACGGGAAAGTAAATTAGTATTGCTGCAAATAGCTAAATTATATTTATT1330.13259558347041522No Hit
CGTATGGGTAGAGCAGAGATCATAACCAAGGAGAGAGAGGTCTCAGAATGGA1330.13259558347041522No Hit
AGCTAAGGGAAGAAGTGTTACCTTATTAAAATGACCACCATTCTAAACCATT1320.13159862419620158No Hit
AGTAGTGGGAGAATTTTGTATCCAGCAAATCTAAGTTTCGTAAATGAAGAAA1310.13060166492198794No Hit
TTGTTAGGGATCCTACTTTTAACAAGCTGTTCTGAAGCTTAATCCAGTTACA1310.13060166492198794No Hit
TAGCTAGGGGCCTCAGGTGGTTCATCCCTTTTGGCACTCATGGTGCCACTTG1300.1296047056477743No Hit
AACTAAGGGGCTCTCACTTTAATTTCATCCTGTCATTAGTAGACAAACGCTT1300.1296047056477743No Hit
CACTAGGGGAAGCTCATGTTTTTAAAGATCGTTTATTGAGATGATTTTGAAA1290.12860774637356065No Hit
TGAATAGGGACACCAACCAGCTTTTCACTTTCGTGGTTTCGTCACGGTTCTG1280.12761078709934698No Hit
CACAGCGGCTGGCGCTGGGATCTGTAATGGTGGAGAAAATGTTTTGTTACTT1280.12761078709934698No Hit
TAGTTGGGGGCTTTTCCCATTGTGATAAACTGGTTCCAGGCTTCACGAGTAC1270.12661382782513333No Hit
TCACAGGGGGAAATAAAAAAATTTAAAAATTTGACTTTATTGGAAAAGTACT1260.1256168685509197No Hit
AGCAATGGGTGAGGCATGAACTTGACCTACGTTCCTCAGAGCCGAGTTTCTA1260.1256168685509197No Hit
GATCTGGGGAAAGCAATATTATTTCCAAGTATATTAATTTGTTAGAAAACTT1240.12362295000249239No Hit
GCATAAGGGCGATCTAAATACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC1240.12362295000249239No Hit
AGAAGCGGGGTGTCTCCATGAGAATCAAATACAGAAACGTGAGCAAATGACG1240.12362295000249239No Hit
TGTGGTGGGACTACTCATTAGCGCACTGGCTAAGCTGCCTTTGCCTTCTCGT1240.12362295000249239No Hit
TGAAGGGGGAGATCTAAAATCTTCACTACTATGTAACCGTAGGTAGGTTGTC1240.12362295000249239No Hit
CCTACCAGTTGTATTGTCTCCAACTAGTTCACCTTTCTGAGCCTCCAGGGCC1240.12362295000249239No Hit
AACGCTGGGCGCTCTTCCGATCTGACTTGGGGAATCAAAACGCATTGAGTGG1230.12262599072827875No Hit
ATCAACGGGGATCTTTAGAAGGGGGTTGAGGTAGAGGGAGCTACAAAGATTA1230.12262599072827875No Hit
AATACTGGGTGGCTGAATCATCCGCTACCTTCACGCCAATGGCGCCTCAATA1230.12262599072827875No Hit
GAATGCGGGTCGCCGGGATATGGGAAAAATTGAATTTTGGAAACAAGGACCA1230.12262599072827875No Hit
CGGTCCGGGGAAGTAGATGCTGATGGTAATGGCACAATTGACTTCCCTGAAT1220.1216290314540651No Hit
TGAGCTGGGGAGTTTTCCTAACCTATTGATACTGCTTATACATGTAAGCAAG1220.1216290314540651No Hit
GGCTCAGGGATAGGAATTGTTAAGAAACAAGGTTTTTGTTTTTTGTGTGCTG1200.1196351129056378No Hit
GCGTGAGGGGCACAGGAAGCTTTCTGCATATTCTTTCAAGAGATTTCGAGGT1200.1196351129056378No Hit
AGCGAAGGGGAGCATTAAAAATCATCAGCATTTAACTGAGACCCCACTATAG1200.1196351129056378No Hit
GCTACAGGGGTATTAAGCTATTTGCTTCTTTCTCTCTCTCTTTTTTTTTTTT1200.1196351129056378No Hit
TACACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1200.1196351129056378No Hit
AAGGCCGGGATTTGCCAAAAGGAGGCACTTTTTATTTAAAATTTGAGACTAA1200.1196351129056378No Hit
AAAAAGGGGATGTTGATAAGGTGGAAGAAGAGATTTTTAAAACATTTTATTA1190.11863815363142416No Hit
AATCGAGGGAGCATTCAATTATGCCAGTTCCATTAGAATACATGAAAGAACT1190.11863815363142416No Hit
AGCATAGGGGGCCATACAGCAGGCCACAATCTGATGACCCTTCTTCTAAACA1190.11863815363142416No Hit
TTAGGGGGAGGCATATTCTTTTCTCTTGCTATCCCCTCTACCTGAAATGATC1180.1176411943572105No Hit
TGTATAGGGATCCCTGATACAAACTGTTGCTAATTACCATCTTTATGGCCTG1180.1176411943572105No Hit
GAAATGGGGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTG1170.11664423508299686No Hit
GCTGGGGGATTTTTCAGACTGGCCATTGAAGTTATATACCTAAGGATAGTGC1170.11664423508299686No Hit
TACTATGGGACATTACCTATGAGAAAGACCCTGTCTTGTACATGTACCAACT1170.11664423508299686No Hit
GTATTGGGGAATAAATATAAATGAGATAAATAAAAGAATACATATTTTACCC1160.1156472758087832No Hit
TACTAAGGGTGAAATGGGAACAAGCAGCCAATTTTCACCTTAAATTTTACTT1160.1156472758087832No Hit
TGTGCTGGGAAGCTGTGTAGATGCCCCATAGAGGACTCTCTCATCCGTGGGC1150.11465031653456956No Hit
TGTCCAGGGAGGCTATCTTTCAGTGGCCATTACTATGTTTCGTACACATGCT1150.11465031653456956No Hit
TGTACTGGGATAATTGGTGTCCCTGGAGATAAGAATGAAAATAATTAAATAA1150.11465031653456956No Hit
AAAAGTGGGACCTAGAATAAATATAAAATGGGGGAAAAATGTGACAGACAAG1150.11465031653456956No Hit
GAATCGAGGGATGAAAGATTAGAAGCCTGGAATAATCAGGCTTTTTAAATGA1150.11465031653456956No Hit
CTTACTGGGCTTGCAGTGGTATCTTTTCTTTGGGTTACAGTGTTGTTCATCC1140.11365335726035591No Hit
ATAAGGGATAGTGTTTTAGTGATGACTGAGAAAATAGGTTGAGTTTTTTTAA1140.11365335726035591No Hit
GGGTCTGGGACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAAT1140.11365335726035591No Hit
GTCTAAGGGACTTGAAGTGGTTTGTGTTCTAAATATATTAACATGCTCAGGG1140.11365335726035591No Hit
CGCGCTGGGGTGATTTTTCCTTTTAATTTTATTGCTCAGAAATGAGGCATAT1140.11365335726035591No Hit
TGTTTAGGGGTGAGAGTTAGTGCCAGATGCTTGTCACATGGGAGGCCATCGA1130.11265639798614226No Hit
GTGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1130.11265639798614226No Hit
AGTTTCGGGTACTGAATACTGTAGGTAGTTGTAACACAATGGTGTTTGTGTA1130.11265639798614226No Hit
ACACAAAGGGAGAAAAGGAAGGAAATAATAAAGATAAAAATATAAATAAGTG1120.11165943871192861No Hit
ATGATGGGGGCATTCAGAACTCTACCACGAGCTCAACCAGAAGTTCCACACT1120.11165943871192861No Hit
CTAACAGGGATATCTTCTGATGTGGAGATTTGAGAAGCATTTGTATTGGATG1110.11066247943771496No Hit
ATCGTGGGGAGCCGCGGGATACATATATGGATGTATTTATAATGACATATAA1110.11066247943771496No Hit
GCAAAGGGGATCCTCTTAGTTCTCCCATTGAGATTTCACTGCTGGACTAAGA1110.11066247943771496No Hit
ACACCAGGGACATTATCTGCTAGCACCATCTGCTAGACTTGAAAAATCAAAA1110.11066247943771496No Hit
ATCTTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1100.10966552016350131No Hit
GCAATAGGGAATCTGAAAAATCTGAAACAGCATCCATAATCTGAAAAATCTG1100.10966552016350131No Hit
TGCTAAGGGGAGGACATCCACCAGTACAGGGAGCAGATCAAACGGGTGAAGG1100.10966552016350131No Hit
TCGTTAGGGATGTATTGCAAACCAGAGATCTCAGTCAGATCCCCTAAGGGGA1090.10866856088928767No Hit
GTAAAGGGGTGTTTTTTGTATGATATTTTTTCTTTCTAATTAAAGATAAATA1090.10866856088928767No Hit
AATTTTGGGGACGGCTCTTCCGATCTTAATCAGGGCATTCAGGCACTTGGTC1090.10866856088928767No Hit
AGACATGGGGCTACAGAGCAGGAGTTGCCGCAGCCCCAGGCTGAGACAGGGT1080.10767160161507401No Hit
TACCTGGGGAAAGGCATCGAGCCTTCCGCATCGCTGATCCAGTGGTTCTGGG1080.10767160161507401No Hit
TATTTCGGGGGTGGGGATGTACCTTAAGTTTCCTTGTGGAATAATAGACTTT1080.10767160161507401No Hit
GTTTGCGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTG1080.10767160161507401No Hit
AAGCTCGGGATATACAGACAAATGATTGCATGAGGCCATGTTTATATGTGTG1070.10667464234086037No Hit
TGAACAGGGAGTACTGAATTTATCCATAAACTCGAAGCTCTGCTGCAAAGAG1070.10667464234086037No Hit
CTGTTTCTGGGCCCAAGAAACTGAGGAATGTTTAATATGAAAACATACATCG1060.10567768306664672No Hit
GTTTAAGGGGACATGTTTTCTGTACAGTCAGTTTCATTAATAAGTAAGGAAG1060.10567768306664672No Hit
CGACACGGGGGGAATTTATCATCTCGCCATAAATATAAAACTTAAGCAACAA1060.10567768306664672No Hit
AAGTTTGGGGCCACCTACCTGCCCTGGAGTGGGCCTGGAATATCTCCGGATA1060.10567768306664672No Hit
TGTATAGGGATTCACCTATGAAATAACCTTTCTTCTTACGAAAACGGAGTCA1060.10567768306664672No Hit
CATACAGGGGTGCGTGTCAACCCCACCGACTGCTGCAAACAGTGTCCAGTGG1050.10468072379243307No Hit
TTAGAGAGGGACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCA1050.10468072379243307No Hit
CAATTTGGGGGAAGAACAAAGAATCCAAAGACCCTGCTGACGAGACTGAAGC1050.10468072379243307No Hit
TCCACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1040.10368376451821942No Hit
TGTGCTGGGAATGAAACAATGTATTTTGAATGCTGAAAATGAACAGTTTGAA1040.10368376451821942No Hit
CAATAAGGGATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAA1040.10368376451821942No Hit
CCAAGAGGGGCTCCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGT1040.10368376451821942No Hit
TATTCGGGGACAAAAACACTGGCACTTGAATGTTGAATGTCACCGTATGCGT1040.10368376451821942No Hit
CAGCCAGGGCGTGGTCTGTGCAAACACTTCGTGGTTCTATATATCAGCAGCA1040.10368376451821942No Hit
CCCCACTGCAGATCCTCCAGATGACTGACCTACTCTAAAGCCCACTGCTCAG1040.10368376451821942No Hit
TAACTTGGGATACAAATGCAAGTAAACAAGCCAATATTTCAACTGTTCCCAT1040.10368376451821942No Hit
TACGCGGGGAGGCTTTCCCCTTTCTTTCTGTTCTAGCAGCAGGAAAAGTTTC1040.10368376451821942No Hit
ATTCATGGGATCTTCCTAGTATGATGGAGAGAGAGGTTGAAAAATCAATAGA1040.10368376451821942No Hit
TCAGCTGGGTGTTTGAAAGCCACTGGATCCGATTTGGAAGCAATATCATCAT1040.10368376451821942No Hit
CAATAGGGGATGCTGAATCAATGTTTCTCAGCCCTTAAATGGCCTTTCTGTG1040.10368376451821942No Hit
ATGTCAGGGACCAACTTGGATTAACATACTGGTTCATGGGTGAAGAGACAAG1040.10368376451821942No Hit
TGGCCGGGCTGACTCCACATACTGCTACAAAGAAATACATCGTTGTTTATAT1030.10268680524400578No Hit
ATTAATGGGGATGCTGGGATGATTGTAACCCTACACACTATTCAATAAGTAG1030.10268680524400578No Hit
TACCGTGGGATCAGTCAAGATCCCACACAAGAAACACAACCAATAGGATACT1030.10268680524400578No Hit
ACATTAGGGAGGGCTCACTGCAGCCTTTACTTGGGATTTTTAAATTATTGTG1030.10268680524400578No Hit
CGAAGGGGGAGATACGTGATTGTTAATGATTTATTTTCCTTTCCTTTTCTCT1030.10268680524400578No Hit
GCAGTGGGGGACAGAGCAAGACTCCATCTCAAAAAATACATAAATAAATAAT1030.10268680524400578No Hit
ACAAGGGAGGCAGAAGACCAGAGAATCTGAACCCTTGAGCAAATCTAAACAT1030.10268680524400578No Hit
ACTATGGGGACTGCTTTGAATTGGATGAATGAATTTGAGAAGTGGCAAGAGG1030.10268680524400578No Hit
TTTGCTGGGGGGAGTGTAAATTGGTTCAGCCATTGTGGAGAGCAGTGGGGTG1020.10168984596979212No Hit
AGAGAAGGGACAAATACATTGTCTTAACAATGAAGTCTATGTACATTTGATT1020.10168984596979212No Hit
CAGTTAGGGAAGCTACCTTGTCATATGTGTTGATTCTTGAAAACTGGGACGT1020.10168984596979212No Hit
TTCTCAGGGAGAGACTAGAATTGTGGAGGTTGGGACCTTGTAACTCCAATAA1020.10168984596979212No Hit
CTACACGGGATTGCACTTTACATAAGACAGGCAAACCACAGGTTCTTGTGGA1020.10168984596979212No Hit
CAGTCTGGGGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTA1020.10168984596979212No Hit
TATTCAGGGGAGCTGAAAACTGATTTACTGAGATCAGCTGTGATAAAAGAAA1010.10069288669557848No Hit
GATTAAGGGTGGTTGCTGATGTTAGATTATTGTTAAACTGTAATTACTAATT1010.10069288669557848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)