FastQCFastQC Report
Sat 31 Dec 2022
SRR5849663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99865
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT6290.6298502979021678No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC4800.4806488759825765No Hit
CACAACGGGATATCAAGCAGGGATGGCAAACTGAAGAGCTGAGGAAATGATG1520.15220547739448254No Hit
ACAAGGAGGGACAAGCTGATGGGAGAGAAGAATAGAGCAGATTGCAGTAGGA1400.14018925549491812No Hit
AACGCTGGGCGCTCTTCCGATCTGACTTGGGGAATCAAAACGCATTGAGTGG1370.13718520002002704No Hit
GCACCTGGGCTTCTGATGGGAAATCTTATGATAATGCATGCCAAATCAAAGA1370.13718520002002704No Hit
GAAATAGGGATTGGAAATCTCAGATGAAATACATTTCTAGTAGTACCACCAG1360.13618384819506335No Hit
TACCTGGGGAAAGGCATCGAGCCTTCCGCATCGCTGATCCAGTGGTTCTGGG1350.13518249637009963No Hit
GCTACAGGGGTATTAAGCTATTTGCTTCTTTCTCTCTCTCTTTTTTTTTTTT1310.13117708907024483No Hit
GTGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1300.13017573724528114No Hit
AGTAGTGGGAGAATTTTGTATCCAGCAAATCTAAGTTTCGTAAATGAAGAAA1290.12917438542031742No Hit
AGCAATGGGTGAGGCATGAACTTGACCTACGTTCCTCAGAGCCGAGTTTCTA1290.12917438542031742No Hit
AATACTGGGTGGCTGAATCATCCGCTACCTTCACGCCAATGGCGCCTCAATA1280.12817303359535373No Hit
TACTATGGGACATTACCTATGAGAAAGACCCTGTCTTGTACATGTACCAACT1280.12817303359535373No Hit
ACAAGGGAGGCAGAAGACCAGAGAATCTGAACCCTTGAGCAAATCTAAACAT1280.12817303359535373No Hit
GTCAGCGGCGCAGCATGCACCAAGGTTATTCGTTATTTACTAGTTCATTCAC1270.12717168177039No Hit
ACTATGGGGACTGCTTTGAATTGGATGAATGAATTTGAGAAGTGGCAAGAGG1260.12617032994542635No Hit
CGAGTAGGGAGGATTAAGAAACTCAGTCAAAACTGCACAACTACATGGAAAC1250.12516897812046263No Hit
TTGTTAGGGATCCTACTTTTAACAAGCTGTTCTGAAGCTTAATCCAGTTACA1240.12416762629549893No Hit
ATCTTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1240.12416762629549893No Hit
AAGCTCGGGATATACAGACAAATGATTGCATGAGGCCATGTTTATATGTGTG1240.12416762629549893No Hit
TATTTCGGGGGTGGGGATGTACCTTAAGTTTCCTTGTGGAATAATAGACTTT1240.12416762629549893No Hit
AATCGAGGGAGCATTCAATTATGCCAGTTCCATTAGAATACATGAAAGAACT1240.12416762629549893No Hit
ACACCAGGGACATTATCTGCTAGCACCATCTGCTAGACTTGAAAAATCAAAA1240.12416762629549893No Hit
TTAGGGGGAGGCATATTCTTTTCTCTTGCTATCCCCTCTACCTGAAATGATC1230.12316627447053521No Hit
ATCAACGGGGATCTTTAGAAGGGGGTTGAGGTAGAGGGAGCTACAAAGATTA1230.12316627447053521No Hit
TCTTTAGGGATCCAAGTCTACCAAGGTAACTAAGTAATGGATGTTTTCTCTC1230.12316627447053521No Hit
CGAAGGGGGAGATACGTGATTGTTAATGATTTATTTTCCTTTCCTTTTCTCT1230.12316627447053521No Hit
GAAATGGGGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTG1220.12216492264557152No Hit
CTTACTGGGCTTGCAGTGGTATCTTTTCTTTGGGTTACAGTGTTGTTCATCC1220.12216492264557152No Hit
GTTTAAGGGGACATGTTTTCTGTACAGTCAGTTTCATTAATAAGTAAGGAAG1220.12216492264557152No Hit
AAGGCCGGGATTTGCCAAAAGGAGGCACTTTTTATTTAAAATTTGAGACTAA1220.12216492264557152No Hit
CATACAGGGGTGCGTGTCAACCCCACCGACTGCTGCAAACAGTGTCCAGTGG1200.12016221899564412No Hit
TTAGAGAGGGACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCA1200.12016221899564412No Hit
AACTAAGGGGCTCTCACTTTAATTTCATCCTGTCATTAGTAGACAAACGCTT1200.12016221899564412No Hit
CACTAGGGGAAGCTCATGTTTTTAAAGATCGTTTATTGAGATGATTTTGAAA1200.12016221899564412No Hit
GATTAAGGGTGGTTGCTGATGTTAGATTATTGTTAAACTGTAATTACTAATT1200.12016221899564412No Hit
TCTAGCGGGCAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAG1200.12016221899564412No Hit
GCTGTTGGGATGGTTTTATAACTCTCTCATTCATATTCAGACACAATGCAAA1190.11916086717068042No Hit
GAATGCGGGTCGCCGGGATATGGGAAAAATTGAATTTTGGAAACAAGGACCA1190.11916086717068042No Hit
TGCCATGGGATGCCTACATCAAGAACTTAGAATGATCACAAATTTACAACCT1180.11815951534571673No Hit
GTCAAAGGGGTAGTTTGCTTTTGATTTCAAAATGCCACCTCTTCTGATTCCA1180.11815951534571673No Hit
TACACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1170.117158163520753No Hit
TGAGCTGGGGAGTTTTCCTAACCTATTGATACTGCTTATACATGTAAGCAAG1170.117158163520753No Hit
TGAGGCGGGATAGTAGCTATACAAAATATATCTCTAGGCCCTAAAAATTTGC1150.11515545987082561No Hit
AGCGAAGGGGAGCATTAAAAATCATCAGCATTTAACTGAGACCCCACTATAG1150.11515545987082561No Hit
TGTCCAGGGAGGCTATCTTTCAGTGGCCATTACTATGTTTCGTACACATGCT1150.11515545987082561No Hit
AGCTAAGGGAAGAAGTGTTACCTTATTAAAATGACCACCATTCTAAACCATT1150.11515545987082561No Hit
ACATTAGGGAGGGCTCACTGCAGCCTTTACTTGGGATTTTTAAATTATTGTG1150.11515545987082561No Hit
TACGCGGGGAGGCTTTCCCCTTTCTTTCTGTTCTAGCAGCAGGAAAAGTTTC1150.11515545987082561No Hit
TGTAGTAGGTATTGTGACTTCATAATTATTTGGAATAAGATTGGTGATGTTG1150.11515545987082561No Hit
AATACTGGGATATTGTCTCTTGTAAAATCCTTACTCTCTCTTTCTCTGCCCA1150.11515545987082561No Hit
GGCTCAGGGATAGGAATTGTTAAGAAACAAGGTTTTTGTTTTTTGTGTGCTG1140.11415410804586192No Hit
CACAGCGGCTGGCGCTGGGATCTGTAATGGTGGAGAAAATGTTTTGTTACTT1140.11415410804586192No Hit
ACAGGGCAACCTAAGTGTCCATCAACAGATGAATGGATAAAGAAAATGTGGT1140.11415410804586192No Hit
GCATAAGGGCGATCTAAATACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGC1130.11315275622089821No Hit
GCAAAGGGGATCCTCTTAGTTCTCCCATTGAGATTTCACTGCTGGACTAAGA1130.11315275622089821No Hit
GCTCGGGGACTTCTCTGAATGTCCAGTTGGAAAGATGTAGAGTTGTTGCCCA1130.11315275622089821No Hit
TGAATAGGGACACCAACCAGCTTTTCACTTTCGTGGTTTCGTCACGGTTCTG1120.11215140439593452No Hit
GTATTGGGGAATAAATATAAATGAGATAAATAAAAGAATACATATTTTACCC1120.11215140439593452No Hit
TCATCAGGGATTGAAGATGATTTGGGTTTATTTTACAGCTATATAAAACATA1120.11215140439593452No Hit
GTCTAAGGGACTTGAAGTGGTTTGTGTTCTAAATATATTAACATGCTCAGGG1110.1111500525709708No Hit
ACTAAAGGGACATGAAAGTATGGGCACCAAAACGTTAAAAGTGGTTTTGTTG1110.1111500525709708No Hit
ATGTCAGGGACCAACTTGGATTAACATACTGGTTCATGGGTGAAGAGACAAG1110.1111500525709708No Hit
AGATTCGGGAAACAAGAGTATTTTTATGAAAGAAAAGGACAAACCTATAAAT1100.11014870074600712No Hit
TACACAGGGGTCATGAAAAGTCCAGTGGAGAAAATCTCAATGCTTACTGTTA1100.11014870074600712No Hit
GCTGGGGGATTTTTCAGACTGGCCATTGAAGTTATATACCTAAGGATAGTGC1090.1091473489210434No Hit
ATTACTGGGATCCCCTTGGAGTGCCATGTATAAGTTGGGCTATTAGAGTTCA1090.1091473489210434No Hit
GCAGTGGGGGACAGAGCAAGACTCCATCTCAAAAAATACATAAATAAATAAT1090.1091473489210434No Hit
GGGTCTGGGACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAAT1080.10814599709607971No Hit
ATGCCTGGGAAAAAACTCACCAAAATGTCTCCATTTGAATTGTATTGATAAT1080.10814599709607971No Hit
TCAGCTGGGTGTTTGAAAGCCACTGGATCCGATTTGGAAGCAATATCATCAT1080.10814599709607971No Hit
AATTTTGGGGACGGCTCTTCCGATCTTAATCAGGGCATTCAGGCACTTGGTC1080.10814599709607971No Hit
TGTATAGGGATCCCTGATACAAACTGTTGCTAATTACCATCTTTATGGCCTG1080.10814599709607971No Hit
TACCGTGGGATCAGTCAAGATCCCACACAAGAAACACAACCAATAGGATACT1070.107144645271116No Hit
TACTAAGGGTGAAATGGGAACAAGCAGCCAATTTTCACCTTAAATTTTACTT1070.107144645271116No Hit
TGCTAAGGGGAGGACATCCACCAGTACAGGGAGCAGATCAAACGGGTGAAGG1070.107144645271116No Hit
CGGAATGGGACCAAGACGTCTGCTTTTGTGACAGTCAGAAAACACACCTGGA1060.10614329344615231No Hit
CAGCCCGGGAAGTTTTGAAAGGTTTACATTTCTGCCCTGTTTTATTGAATGT1060.10614329344615231No Hit
GTCGCGGGAGAGCAGCAATCTTTGAAATTAGTCAACAATGCAGTCTTTTTTA1060.10614329344615231No Hit
ACACAAAGGGAGAAAAGGAAGGAAATAATAAAGATAAAAATATAAATAAGTG1050.1051419416211886No Hit
GCGTGAGGGGCACAGGAAGCTTTCTGCATATTCTTTCAAGAGATTTCGAGGT1050.1051419416211886No Hit
AGTAGGGAGTTCTGAGTTTTGACAAGCATATACAGTCATGTAACCACCACAA1050.1051419416211886No Hit
TCAGGCGGGATGCCAAGCCAGACTGACTACAGCCTCCTCTGAACTCTGTTGT1050.1051419416211886No Hit
ATCGTGGGGAGCCGCGGGATACATATATGGATGTATTTATAATGACATATAA1050.1051419416211886No Hit
CAATTTGGGGGAAGAACAAAGAATCCAAAGACCCTGCTGACGAGACTGAAGC1050.1051419416211886No Hit
TACCAGGGGACTTCAAGCTTTACCCTCTTAGTGAAAATAGTCTTAAAGATTC1050.1051419416211886No Hit
AGAGAAGGGACAAATACATTGTCTTAACAATGAAGTCTATGTACATTTGATT1040.10414058979622491No Hit
CGACGGGGGAAAAGATTTAAGTAATTTTGCCATCCTAGGCTTAAATGCAATA1040.10414058979622491No Hit
CCAAGAGGGGCTCCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGT1040.10414058979622491No Hit
ACGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1040.10414058979622491No Hit
GCAACGGGATTTGGAAGAGAGATAGTCTTTTGTTTTTAATAAGTTTCTTACT1040.10414058979622491No Hit
GGGGACGGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTA1040.10414058979622491No Hit
GATCTGGGGAAAGCAATATTATTTCCAAGTATATTAATTTGTTAGAAAACTT1030.10313923797126119No Hit
CCCCACTGCAGATCCTCCAGATGACTGACCTACTCTAAAGCCCACTGCTCAG1030.10313923797126119No Hit
TCACAGGGGGAAATAAAAAAATTTAAAAATTTGACTTTATTGGAAAAGTACT1020.1021378861462975No Hit
TCCACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1020.1021378861462975No Hit
TGTACTGGGATAATTGGTGTCCCTGGAGATAAGAATGAAAATAATTAAATAA1020.1021378861462975No Hit
CGTATGGGTAGAGCAGAGATCATAACCAAGGAGAGAGAGGTCTCAGAATGGA1020.1021378861462975No Hit
CTACACGGGATTGCACTTTACATAAGACAGGCAAACCACAGGTTCTTGTGGA1020.1021378861462975No Hit
AGCTACGGGAAAGTAAATTAGTATTGCTGCAAATAGCTAAATTATATTTATT1010.1011365343213338No Hit
GATTCTGGGAGGATATAACTTATCAGCAACCAAGACTTACAGCATGGTATGG1010.1011365343213338No Hit
AAGCTGGGGAACTACGGGATGGTCAGAAGCACTGAAGCAACCCTGAGCTTTG1010.1011365343213338No Hit
TGAAGGGGGAGATCTAAAATCTTCACTACTATGTAACCGTAGGTAGGTTGTC1010.1011365343213338No Hit
TGTACTGGGCGATCTGCACAGGGTGCACACAACAGATTTTAGATACTTTTTG1010.1011365343213338No Hit
GTCCACGGGGTAGTTTGAAAACCATTGTTCTGAGGAAGGGGGTCCAATCTGG1010.1011365343213338No Hit
TTTGCTGGGGGGAGTGTAAATTGGTTCAGCCATTGTGGAGAGCAGTGGGGTG1000.1001351824963701No Hit
CGGTCCGGGGAAGTAGATGCTGATGGTAATGGCACAATTGACTTCCCTGAAT1000.1001351824963701No Hit
TATGCTGGGAATCTTAGCATGCTTTCTGCTCTTTCTCTTTGGAAGTAGCATT1000.1001351824963701No Hit
ATGATGGGGGCATTCAGAACTCTACCACGAGCTCAACCAGAAGTTCCACACT1000.1001351824963701No Hit
CAATAGGGGATTCTCTATTTTTTAATGTTACTCAGATTTGATATGCTATGCT1000.1001351824963701No Hit
ATGCTGGGGGAGCCAACGTTTACTGACCGTCCTGCGTCTTGGACCAGCCTAC1000.1001351824963701No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)