FastQCFastQC Report
Sat 31 Dec 2022
SRR5849659.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849659.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85511
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT5610.6560559460186408No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC5010.5858895346797488No Hit
TTAGGTGGGACCAGAAAACAGATGCAGCTAGCAAACCGTTTGCGGAGGTACG1380.16138274607945177No Hit
AGCCTGGGTGACGGAGCCAGATTTTGTCTCTAAAAAACAAGAAAAAGAAAAA1320.15436610494556255No Hit
GAACTGGGGGAAGTGAGAGAAAGAAGAGGGAGGCTCGTTCTGTTGCTCGTAT1270.1485189040006549No Hit
AGTAGGGGGTGCATTAAAACTGTTCTCTGAAATTAAGCTTGTCTTGTGAGGA1260.14734946381167335No Hit
AACATGGGGGGCAACAGTGGACTCAGACTTCTGAGGAATTTGGGTTTGTTCC1250.1461800236226918No Hit
AACTATGGGACGACCTTAACAACCCGGACAAGCACGCCAAGCTGGACTTCCT1230.14384114324472874No Hit
AGCGCAGGGACGGCCTCGCCTCGGTATCGCAGCGGGTCCTCTCTATCTAGCT1230.14384114324472874No Hit
GACACAGGGTATTTAATCAGTGTCCCCCCAATAAATCACTTATCCATTTATT1220.14267170305574722No Hit
CTTTTAGGGGGCCCACACTAGACATGGCGCTTGCCAGCGTGTTGGAGAGACC1210.14150226286676568No Hit
GCCAAGCGGGCACTCAGGGCATTGTATCTGATAGCCACTAATGGAACTCCAG1210.14150226286676568No Hit
TTATGGGCGATCTAAAAGCGGGATTGTTTTAAATGCCAACACTGTCTTCTCA1200.14033282267778413No Hit
TCCACTGGGCTGCTTTGCCTGATCTAGTAGCTGTCCACATATTCAGGTAATC1200.14033282267778413No Hit
GTGTAAGGGATGTGAAGTTTACAAAGGAACCAAAATGATAATGGGAGAATTT1190.1391633824888026No Hit
GAAGCAGGGATCCACACTTCTCCAGAATCATGCACCAGACGGTGAAGGAACT1190.1391633824888026No Hit
AGTCTAGGGGGCAGCGTGAGTAGGGAGCCCCGGCTGGTGGACGCCGAGCCTC1180.1379939422998211No Hit
TCTACAGGGAGAGGGAGCGGGCAGGGAAAGGGGGAGAGTGTTCCAGGAATGC1170.13682450211083955No Hit
GGGAAAGGGAAAATAATTCATTTTCTAAGACATTTTTGAGGAGATAATACTA1160.135655061921858No Hit
CAGTTGGGGGACGCTCTTCCGATCTCTAGAAGGGTTATTTGGGCAAGTTTAA1160.135655061921858No Hit
TTCCTAGGGTAAATAAATAAATGAAGTAGAAGAAGAACCAGCAGTTAGGGTT1150.13448562173287648No Hit
GCGTGCGGGCTCCGGGGCCCTGGGAGGCCATGCAGAGAGGCAGAGGTCCTTT1150.13448562173287648No Hit
ATATGGGGGCAGAGTCGCTTAACACAAAGCACCCAACTTACACTTAGGAGAT1130.1321467413549134No Hit
AAAGTGGGGGAGAACATGGTTTAATTATTCGAGTGAAAGGATTTAACCACAA1120.13097730116593187No Hit
GACAATGGGACTTAACTGCATGATTTCTGTTTTATCTACCTCTAAAGCAAAT1110.12980786097695035No Hit
GCAGTAGGGGAATTGAGTTTTCTTTTCTTGATGTTGGTTTCCTTCATATCAC1100.1286384207879688No Hit
AGTTGCGGGTAAGCCGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC1100.1286384207879688No Hit
CGATGCGGGATACTAATGAGAAACCTATGAATGTCAGGAATGCAGCAAGGCC1100.1286384207879688No Hit
CTATAAGGGATCTGAGAGTTTGGGGAGGATATTTCTATAGGGACCTTTAACC1100.1286384207879688No Hit
GTACCTGGGAGAAGAGGAGTGTAGTTGGCATCTTTTCCTTGAAAGATTAATA1090.12746898059898726No Hit
TGCACGCGGGCTGGGGACTGATAAGTGCTTTAAGCAATGTCCATACCCCGTC1090.12746898059898726No Hit
TACATGGGTGAAATTAAATAATGGTTTGAACAGAAAATTCAAACAAGACTCT1090.12746898059898726No Hit
TATTCTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT1090.12746898059898726No Hit
AATTTGGGGAAACATCCTTCTGTACCTTCTTCAATTTTACCCCTTTCCTTAT1090.12746898059898726No Hit
ATTCTAGGGCAATTTGAAAATAAAAATTGAAAACAGTATGCTTTATAACAGC1090.12746898059898726No Hit
GAACACGGGATATTCAGTAATGAAGAATGGTGCCACCACTCAAGCAACAAGC1080.12629954041000574No Hit
TGTTCCGGGGTGAGTCTGCGACAAGGGCCCCACGGGGACGGTGCTCGGCGTC1080.12629954041000574No Hit
GAAACGGGGCTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGGAACAGAGT1070.1251301002210242No Hit
TAACACGGGGGTACATAAATGATTATAGCAGCATTATTCTTAATAGCCAAAA1070.1251301002210242No Hit
ATAAAAGGGACTGAAGTACTAGGTAAATGTTTTTGTTTTGTTTGTTATTTAT1070.1251301002210242No Hit
TTATATGGGATGACATGGAAATTATTGAAGAAGAAGCTTTCTTGGCTTGGAA1060.12396066003204267No Hit
AAATCCGGGCTAGATCTAAAGAGAGAAAAAGACATCAATACAATAATAGAAG1060.12396066003204267No Hit
TTCTACGGGTACGCAGTTTAACTATGTAGATAGATTCCTATTGCACCATAAT1060.12396066003204267No Hit
GATATAGGGCACATATGCAATGCCTGATGGGTTCCTTGCAGCCCAAAATGAT1060.12396066003204267No Hit
GCACAGGGCCGATCTGTCAAGGGTAGACTCAAATGTTGTAAAGCAGGTGCTC1060.12396066003204267No Hit
GTTAACGGGATTTACCTTAAGTAGGTATAACTCTTATGGGATATACAGTATA1050.12279121984306111No Hit
TGATCAGGGTTAAAAAAGCACCGTGGAGAATGGCCCTCTTCAGGAAAGAAAA1050.12279121984306111No Hit
TTGTCTGGGAAACGTGAAGAAAAATAAAAATATAGATTTTCCAGCACTGGGA1050.12279121984306111No Hit
TCAAGTGGGCAAGGAGCAGCGGCACTGGGACATCTCGTCGTCCGAGCGCCTG1040.1216217796540796No Hit
AAATTAGGGATGGCTCCCAGTGGGTATTGGCGATTCTTGTGATGCAGGGCCT1040.1216217796540796No Hit
GTATCAGGGATGGCTCAAAGCCTCTGGGGGCTCAAGGAAGGCTGGGCTGCTC1040.1216217796540796No Hit
CGTCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1040.1216217796540796No Hit
TTTGAGGGGGTCTGAACATCAGCTCCTCCATGACAAGGGGAAGTCCATCCAA1040.1216217796540796No Hit
TCGGTTGGGGATATAGGAGAATTCAGCTTATCTAGACAGTCTTAAGTTTCTA1030.12045233946509806No Hit
ACCAAAGGGGCAACCATGATTTGACAAGTGGAGTTGCATCCCAACTCACTGA1030.12045233946509806No Hit
TTTCAAGGGTTTGAATCTCACCATTTTCCTGTGCATCGGCAAATTTGTTTGG1030.12045233946509806No Hit
GGACTTGGGGGACGAGGCCGTGGGTACGACCGGAAGCCGCAGCATGCTGATC1030.12045233946509806No Hit
GCTTAAGGGTACCACAATCATCTACTTTCTTATTAGAGGACAATTTAGGCAG1030.12045233946509806No Hit
AACCGAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1020.11928289927611653No Hit
AAAACTGGGGGAGCAGCAAAATAAATAATGTGGTACCGGATTCTAAATCATT1020.11928289927611653No Hit
TATCAAGGGGCCTGGAGAATGGCTCTCACTACTCACCTTGTCTTTCAGCTTC1010.11811345908713498No Hit
CTTAAGGGGATTTGTATCTGGCATCACTTACTAACACACGACATGCGGCTTT1000.11694401889815345No Hit
AAATCCGGGGGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCACGGC1000.11694401889815345No Hit
TGTAACGGGTGATAGAAGTAGCATGTTGCTGCCAGAATACAAGCATTGCTTT1000.11694401889815345No Hit
TACCATAGGGGCTGGCAAGGGAGGAGGGCCGGCCAGCATTTGGTGGCCCATC1000.11694401889815345No Hit
GACTTGGGGAAATTGGTGATTACAAGATGAATGTTATCCGGGTAAATGGAAG990.11577457870917192No Hit
TGCCATGGGACAGGTGTGAGCCACCATGCCTGGGCTAAACTCTTTAAGTCTC990.11577457870917192No Hit
TAAAAAGGGGAACTCCTGACCTCAAGTGATCCACTCACCTCGGCCTCCCAAA990.11577457870917192No Hit
TTATGAGGTGCAGTATAGAAGGCAAATGCAATTATAGTCTTTATTATAGTAG990.11577457870917192No Hit
GCGGCTGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG990.11577457870917192No Hit
CTCAGTGGGGGTTTACATGTTCCAATATGATTCCACCCATGGCAAATTCCAT980.1146051385201904No Hit
CATTTTGGGAAGGACAGCTTTGCAGTTCTCCTGGAGTTCGCTGAGGAGCAGC980.1146051385201904No Hit
TTAAAAGGGATAAAATGATGCTTGGCATTTTTAGAGTATCTGCCATATCTAT970.11343569833120884No Hit
CAGTCTGGGCTCCTGTGTACCGGCATCTTGTGTTGGGAATGTTCCCCCCTCC970.11343569833120884No Hit
GTTAAGGGACGAACTTTGCAAAATACTTAAATGACAATTACCTAGAAAATTC970.11343569833120884No Hit
GTCAGGGGAGCCCTAAGGTGAACCAGACTTGGGAAAGATTGTCTTTAAAAAT970.11343569833120884No Hit
TGCTTCGGGATCGGACTGATGCGTGCTTCATTCGAGTTATTGGATCTGAGCT970.11343569833120884No Hit
AAAACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA960.11226625814222732No Hit
ATCCAGGGGCTTGCTTCCTCGCTCCATAGCCCTCAACTGCCCAGGCGCTCCC960.11226625814222732No Hit
ACAAGAGGGATGTTTATTCAGAATCTTCTGTGGGAAAAGAATGTGAGAAACA960.11226625814222732No Hit
TGCAAGGGCATCTCTTGGCTGTACTATAAGAACACATTAATTCAATGGAAAT960.11226625814222732No Hit
CAGCATGGGTTAGCGGAAATGAAGACATTGATCAAAGCTCTGGTGAGATGGG960.11226625814222732No Hit
CCCCAGCCCCTCATCTTCCACATGGAGATGCTGAAGGTGGAGAGCCCTGGCA960.11226625814222732No Hit
CGGACCGGGTGGTTAGAGAAATTTTCTGTCCTAAAAGTAGAAATAGCCCCTT960.11226625814222732No Hit
GTATATGGGATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCACAGT960.11226625814222732No Hit
GCTGATGGGCTGCCACCACGCCCGGCTAACTTTTTGTATTTTTAGTAGAGAC960.11226625814222732No Hit
ACGACGGGGACATTTATTGCTATCCAGTGTCAGAACAGTTAGTTTAGGAAAA950.11109681795324579No Hit
TTGTTTGGGAGCAGAACTCTTACTGGTAGAAAAAATAGACCTGAATTATGTG950.11109681795324579No Hit
TATTCTGGGACCACACATTGGGGGGAAAAAAGACATTTTCCTACACCCACCC950.11109681795324579No Hit
CATTTAGGGGGGCGACGCCACAGAGAAAGATGCATCCAAGAAGTCAGATTCA950.11109681795324579No Hit
ACTAACGGGGGTTTTAAGAATTGAGAAATATCACATTGCCCCTGATGTTTTG940.10992737776426426No Hit
TTGGACGGGTATCTGAAGTTGGGATGTGATAATTGGGTTTTTAAAAATCTGA940.10992737776426426No Hit
TGATTAGGGTGACAAGCTCGGGCCTATGATTCAGAAGTATGATGAGCCCATT940.10992737776426426No Hit
TATCCATGGGACAAATTAAAATGTTATGACTTTGATGTGCATACAATGAAGA940.10992737776426426No Hit
GACGACGGGGCTTCTAAGTTCATTCAGGTTGTTGGCGGAATTCAGTTCTTTG940.10992737776426426No Hit
TCTTACGTCGGGAGTTCAAGACACTTAGTCCTCCACAGTGGGTGGAAGAGTG940.10992737776426426No Hit
ACAATTGGGTTGGAAATGGATATCAGACTATGAAGGTCATCAATGTTATCTG930.10875793757528271No Hit
GGCAGTGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTA930.10875793757528271No Hit
TATTCCGGGAGGTACCATCCATGCATACAATTTGGTCATAGCAAAAGTAATT930.10875793757528271No Hit
CACAGTGGGTCTCAAGTAGGGAGGAGAAATGATGGATCTGCAGCATGGGAGC930.10875793757528271No Hit
TACCATGGGGAAGTTCTGTCACTGGATCTGGTTTCAGTGTCTCAGACCTTGC930.10875793757528271No Hit
TACTATGGGAACATCTCTCCTTGATTGTCATTTCATTCTTTTGCATGTTATT930.10875793757528271No Hit
ATTGCCGGGCCGATCTTTGGTAGGGATCCTCAGGTCTGCAACCAAAGATTCA930.10875793757528271No Hit
GCTGCCGGGGATGGGAGGATGCTGTTACTAGGAGAGTTTTGAGATACTGCAG930.10875793757528271No Hit
TTGAGTCGGGGAGGACGGGACCCCAGGGCAGCGAAGCAGAATGGCCAACATG920.10758849738630118No Hit
ACCACCGGGCTTTGGGAGGCTGAGGCAGGTGGATTGCCTAAGCTCAGCAGAC920.10758849738630118No Hit
TTACAGGGGAAGAAAAGAACTGGTTTATTTTAATGAAAAGATTTGATTCCCC920.10758849738630118No Hit
TGCTAAGGGGGGATCATTTAGAGATGGGGGTTAGGGAGAGTAGGAGTAACTC920.10758849738630118No Hit
TTGAAAGGGGTCGCGCCGTGTTTTCTTCACCTCAGAAGATTGAAGGTTTTAA920.10758849738630118No Hit
TTTACTGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT920.10758849738630118No Hit
ATTACGGGACTCCGTACAGTATGAACATTAAGACAACAGCCAGTCTATCTAC910.10641905719731964No Hit
TTTGAGGGGATGCCAGGAAGTCATCATTTCACATGCATTAGTTGCAGAAGTT910.10641905719731964No Hit
GCGACAGGGGTATACTTTTTTATAATTAATGACTAAATGATCACTTTGGAGG910.10641905719731964No Hit
GCGTCCGGGCAAGTTCAAGATCCACATCCCAGGTGGCCTCAGCCCCGAGTCC910.10641905719731964No Hit
TATGCTGGGATTTAAAAATAAGTCAACAGTAAACCACTGAAAAAATATATGT910.10641905719731964No Hit
CAGTTTGGGGGAAATTAAAAGATCTGGTTAGAGTAAAAAGTTCTTTTTAAGG910.10641905719731964No Hit
AATCAGGGGGGGCAAAAAGTGAGAAAGTAAGACTGTGGGAGGTGACTGTTAC910.10641905719731964No Hit
TGACATGGGCAAATAGCAAGAGAAGTGCATTTAGCTTCTTATGATTTGCACA910.10641905719731964No Hit
AATTAAGGGAAGAGAGATATATAAATCTCTGAATCTATTTGTGGATATGTCC910.10641905719731964No Hit
TTAAATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT910.10641905719731964No Hit
AACGTGGGCAAAGTTCTTAAGGACCAACCTCCTAATAGTGTGGAAGGTCTTC900.10524961700833811No Hit
GAAGCAGGGGGAGCTGCGCTGCTCGTTTCCAACCCTAGGTGCTGACCTCTAG900.10524961700833811No Hit
ACGTGGGTAGATATATGAAAAACAAAAAGCAGGAAATTAAATCATACCACCA900.10524961700833811No Hit
GTTAGCGGGTTGATGGAAGTAGTTTCCAGGAAAATAAACTGTCCTCAAAAAC900.10524961700833811No Hit
CAGTACGGGAAGACTATAGCTGCAATAGATAGTGATTCCTTTGATAGATCTG900.10524961700833811No Hit
CACGAAGGGATGAAATCAACATTTCAAATTGGTAATAGAATGACCTTATTTA900.10524961700833811No Hit
TACCCTGGGGATAGTCTGAGGCTATTGACTTAAACCAATAACTGTACTTTAT900.10524961700833811No Hit
CTGTCTGGCGATCTGTATTCGGGGAACAAAATATAATTATTTATGGTACAAT890.10408017681935657No Hit
TTCGGAGGGGTCATTACCAGGCACGCGCAGGAAACATGGCGGCGGCGGGTGT890.10408017681935657No Hit
ATACCCTAAGCAGATAGCAAAGAAGATAATGGAGGAGCAATTGGTCATGGCC890.10408017681935657No Hit
TCTGCCGGGTTGGTCAACTTACTGGTAACACCAGAACAAGAAAGCAAAAAAG890.10408017681935657No Hit
TAAAAAGGGAAAGACAGCCAGTTCGAAACTGATATAGTACAACGAATGGAAC890.10408017681935657No Hit
TAGCCGGGGAGGTGGAAATAGGAGTCACTGATTTGAGTCATTTGTTTTGTAA890.10408017681935657No Hit
GATGCAGGGAAGCAGCCTTATGAAAAGAAGGCTGCGAAGCTGAAGGAAAAAT890.10408017681935657No Hit
CAAATAGGGTCTGTGAGAGGGCAGTATTACCTGCCATGAAGAGATATTTTAT890.10408017681935657No Hit
CCTACAGGGGTAATATTTTTTTTTTCCATGTAGAACAAATTATGGGCAGAAA880.10291073663037503No Hit
ACAACGGGAAGGAAATAGCAAATTGGGGTGGGGGGAATGTAGGGCATTCTAC880.10291073663037503No Hit
TATAGTGGGATTGACCCATCATCTGGTTTGGCATTTTTTGGACCATATACAA880.10291073663037503No Hit
TCGTTAGGGATGTTCTATAAATTCAGAGGAGTAGAAGATTACAAAATTCCAA870.10174129644139351No Hit
GCAGCAGGTCCAAGATGGCTAACCAAGATGGCTAATTTAGCCACATTGAATA870.10174129644139351No Hit
TTTGGCGGGGTGGACAAGAGTACGGGAAAAGAGAACAAGATTACTATCACTA860.10057185625241198No Hit
CAAGCAGGGGTCTGCCTTCACTGATATAAATCATTTCAATGTTAATAATAAT860.10057185625241198No Hit
TCTTACCGGGAACTTTGCAAAACATTCGTTTGAATCCCACATAAATCACATG860.10057185625241198No Hit
TAACGTGGGGTTATGAAATATTATATTGCCATCAAAAAGAATGAATTAGCCA860.10057185625241198No Hit
AAAAGGTGGGATCTAACTCACCCTCAGACTGTCACTGGAACACCTGCATGAA860.10057185625241198No Hit
TGTCTGGGGCCAGGACTAGAAACGCCTCCTCCTCCTCTTCCAGGATCCCGGC860.10057185625241198No Hit
TCTTAGGGGTAGTGAAAAGCCTAATTACAGAAAATTGTGCAGATACTAGTGA860.10057185625241198No Hit
TGTTTTGGGAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGA860.10057185625241198No Hit
CACTCTGGGAGATTTGGTTGCATAAGTAAAACATTCTCATTTATAAAAGACA860.10057185625241198No Hit
ACTCATGGGGTAGCTAACCTTTGTTGGAGTCAGGAAAAGCTTCTGGGACAAA860.10057185625241198No Hit
CAATCCGGGTGGACTTGAATGGCACCTGGAGCCCCTTCATTTTGGCCAATTT860.10057185625241198No Hit
GCCGTTGGGATTGTCCCCAGGACAGATAGCAGGGAGGGAAGGTAGAGAATGG860.10057185625241198No Hit
AACAATAGGTATGGTTTGTATGTGAACAGTACTTAATGTGAAAATTACTTTT860.10057185625241198No Hit
AATCGGGAGACCAATTGCAAAACTAAGCAAAATAAGAAAAGAGAAGATCCAA860.10057185625241198No Hit
TAGAAAGGGATCAACAAGTATTTCAATAATACCTCCTCCGCTCCACCTGCTG860.10057185625241198No Hit
AATCAGGGCGTCTGGATCCAAATGTTCAGAAATGGGATGTAACAGTATTAGA860.10057185625241198No Hit
CTCGCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC860.10057185625241198No Hit
CATCCTGGGGGGGGAGGTGGATGTCAGATCTGGTAGGTTGCAGCAGAGAAAA860.10057185625241198No Hit
GGAATAGGGTGCACAGGGGAGAGCTGTAGAGGTTACAGTAGCATCACCAGCC860.10057185625241198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)