FastQCFastQC Report
Sat 31 Dec 2022
SRR5849657.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849657.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84249
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGCCGTCT5340.6338354164441121No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCATGATCTCGTATGC4410.5234483495353061No Hit
TTAGGTGGGACCAGAAAACAGATGCAGCTAGCAAACCGTTTGCGGAGGTACG1370.16261320609146696No Hit
AGTAGGGGGTGCATTAAAACTGTTCTCTGAAATTAAGCTTGTCTTGTGAGGA1360.16142624838277012No Hit
AGCCTGGGTGACGGAGCCAGATTTTGTCTCTAAAAAACAAGAAAAAGAAAAA1350.16023929067407328No Hit
CTTAAGGGGATTTGTATCTGGCATCACTTACTAACACACGACATGCGGCTTT1240.14718275587840807No Hit
CTGCCTGGGGTTTCTTTTGCCACCTACTCCTAACTTTTTGTTTGGTTTTTAA1240.14718275587840807No Hit
TTGAGTCGGGGAGGACGGGACCCCAGGGCAGCGAAGCAGAATGGCCAACATG1220.14480884046101436No Hit
TAAAAAGGGGAACTCCTGACCTCAAGTGATCCACTCACCTCGGCCTCCCAAA1210.14362188275231755No Hit
GAACTGGGGGAAGTGAGAGAAAGAAGAGGGAGGCTCGTTCTGTTGCTCGTAT1200.1424349250436207No Hit
GACTTGGGGAAATTGGTGATTACAAGATGAATGTTATCCGGGTAAATGGAAG1180.140061009626227No Hit
GCGGCTGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1180.140061009626227No Hit
TATTCTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT1180.140061009626227No Hit
ACCAAAGGGGCAACCATGATTTGACAAGTGGAGTTGCATCCCAACTCACTGA1170.1388740519175302No Hit
AAAACTGGGGGAGCAGCAAAATAAATAATGTGGTACCGGATTCTAAATCATT1160.13768709420883335No Hit
TTGTCTGGGAAACGTGAAGAAAAATAAAAATATAGATTTTCCAGCACTGGGA1140.13531317879143964No Hit
GCTGATGGGCTGCCACCACGCCCGGCTAACTTTTTGTATTTTTAGTAGAGAC1130.13412622108274283No Hit
TTAAACGGGATTTTTGTATGTGTTAAAAAGACTACTATATCACAATTAAAAG1110.13175230566534915No Hit
GAAACGGGGCTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGGAACAGAGT1110.13175230566534915No Hit
GTCAGGGGAGCCCTAAGGTGAACCAGACTTGGGAAAGATTGTCTTTAAAAAT1110.13175230566534915No Hit
TTCCTAGGGTAAATAAATAAATGAAGTAGAAGAAGAACCAGCAGTTAGGGTT1100.1305653479566523No Hit
AGCGCAGGGACGGCCTCGCCTCGGTATCGCAGCGGGTCCTCTCTATCTAGCT1100.1305653479566523No Hit
ATATGGGGGCAGAGTCGCTTAACACAAAGCACCCAACTTACACTTAGGAGAT1090.12937839024795547No Hit
GACAATGGGACTTAACTGCATGATTTCTGTTTTATCTACCTCTAAAGCAAAT1090.12937839024795547No Hit
GAAGCAGGGATCCACACTTCTCCAGAATCATGCACCAGACGGTGAAGGAACT1090.12937839024795547No Hit
TGTCTGGGGCCAGGACTAGAAACGCCTCCTCCTCCTCTTCCAGGATCCCGGC1080.12819143253925863No Hit
TGTTCAGGGTCCGATCTGATTACGGGTAATGGCAAAAACTGCAATCACTTTT1080.12819143253925863No Hit
CAGTCTGGGCTCCTGTGTACCGGCATCTTGTGTTGGGAATGTTCCCCCCTCC1070.1270044748305618No Hit
ACATAAGGGACTTCTTTTTGAAATGGAACTATGCAAAGTCTTGAATTTTATT1070.1270044748305618No Hit
GATGCAGGGAAGCAGCCTTATGAAAAGAAGGCTGCGAAGCTGAAGGAAAAAT1070.1270044748305618No Hit
CTTTTAGGGGGCCCACACTAGACATGGCGCTTGCCAGCGTGTTGGAGAGACC1070.1270044748305618No Hit
TGGCCAGGGACATTAGAAGCCAAGCCAATCCTTTTTCTTTTTTTTGGAGGTC1070.1270044748305618No Hit
ATCAAGGGGGGTTCCAAATCTCACAAGCCTCTTTGTATGTGATGCTCTCGCA1070.1270044748305618No Hit
GTGTAAGGGATGTGAAGTTTACAAAGGAACCAAAATGATAATGGGAGAATTT1060.12581751712186495No Hit
TACATGGGTGAAATTAAATAATGGTTTGAACAGAAAATTCAAACAAGACTCT1050.1246305594131681No Hit
AATCAGGGGGGGCAAAAAGTGAGAAAGTAAGACTGTGGGAGGTGACTGTTAC1050.1246305594131681No Hit
TATCAAGGGGCCTGGAGAATGGCTCTCACTACTCACCTTGTCTTTCAGCTTC1040.12344360170447126No Hit
TTATATGGGATGACATGGAAATTATTGAAGAAGAAGCTTTCTTGGCTTGGAA1040.12344360170447126No Hit
GAACACGGGATATTCAGTAATGAAGAATGGTGCCACCACTCAAGCAACAAGC1040.12344360170447126No Hit
TATTCTGGGACCACACATTGGGGGGAAAAAAGACATTTTCCTACACCCACCC1040.12344360170447126No Hit
ATCCAGGGGCTTGCTTCCTCGCTCCATAGCCCTCAACTGCCCAGGCGCTCCC1030.12225664399577443No Hit
TTTCAAGGGTTTGAATCTCACCATTTTCCTGTGCATCGGCAAATTTGTTTGG1030.12225664399577443No Hit
AGTCTAGGGGGCAGCGTGAGTAGGGAGCCCCGGCTGGTGGACGCCGAGCCTC1020.1210696862870776No Hit
TGTTTTGGGAGGCTTCGGAATAATCTCCCATATTGTAACTTACTACTCCGGA1020.1210696862870776No Hit
TCCACTGGGCTGCTTTGCCTGATCTAGTAGCTGTCCACATATTCAGGTAATC1020.1210696862870776No Hit
TGCCATGGGACAGGTGTGAGCCACCATGCCTGGGCTAAACTCTTTAAGTCTC1020.1210696862870776No Hit
GCGTGCGGGCTCCGGGGCCCTGGGAGGCCATGCAGAGAGGCAGAGGTCCTTT1020.1210696862870776No Hit
AATTTGGGGAAACATCCTTCTGTACCTTCTTCAATTTTACCCCTTTCCTTAT1020.1210696862870776No Hit
CTATAAGGGATCTGAGAGTTTGGGGAGGATATTTCTATAGGGACCTTTAACC1020.1210696862870776No Hit
TAACACGGGGGTACATAAATGATTATAGCAGCATTATTCTTAATAGCCAAAA1010.11988272857838075No Hit
TGCTCTGGGAACTTTGAACCTGTGAAATGCTTTACCTTGTTTACAGTTTGGC1010.11988272857838075No Hit
AAAGTGGGGGAGAACATGGTTTAATTATTCGAGTGAAAGGATTTAACCACAA1010.11988272857838075No Hit
AGTTGCGGGTAAGCCGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGAC1010.11988272857838075No Hit
TTAAATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCT1010.11988272857838075No Hit
TAAGCTGGGATCCTGCATACAACAAAAAAACACACTAGCCCCTTCCCCAGGG1010.11988272857838075No Hit
GGCAGTGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTA1000.1186957708696839No Hit
ACTAACGGGGGTTTTAAGAATTGAGAAATATCACATTGCCCCTGATGTTTTG1000.1186957708696839No Hit
AAAAGGTGGGATCTAACTCACCCTCAGACTGTCACTGGAACACCTGCATGAA1000.1186957708696839No Hit
CACTCTGGGAGATTTGGTTGCATAAGTAAAACATTCTCATTTATAAAAGACA1000.1186957708696839No Hit
TGCACGGGTACAGAACGAACGTTGTCTTCACATGTTTTTACAAGATGAAATA1000.1186957708696839No Hit
TCTTACGTCGGGAGTTCAAGACACTTAGTCCTCCACAGTGGGTGGAAGAGTG1000.1186957708696839No Hit
GTATCAGGGATGGCTCAAAGCCTCTGGGGGCTCAAGGAAGGCTGGGCTGCTC990.11750881316098707No Hit
TACCATGGGGAAGTTCTGTCACTGGATCTGGTTTCAGTGTCTCAGACCTTGC990.11750881316098707No Hit
ACTCATGGGGTAGCTAACCTTTGTTGGAGTCAGGAAAAGCTTCTGGGACAAA990.11750881316098707No Hit
GTACCTGGGAGAAGAGGAGTGTAGTTGGCATCTTTTCCTTGAAAGATTAATA990.11750881316098707No Hit
GCTTAAGGGTACCACAATCATCTACTTTCTTATTAGAGGACAATTTAGGCAG990.11750881316098707No Hit
TACCCTGGGGATAGTCTGAGGCTATTGACTTAAACCAATAACTGTACTTTAT990.11750881316098707No Hit
TTATGGGCGATCTAAAAGCGGGATTGTTTTAAATGCCAACACTGTCTTCTCA980.11632185545229023No Hit
TTGCGCAGGAGACAAAACCTGGATTTTTGTGATAAAAGCACAGGATGGTGTC980.11632185545229023No Hit
TGCACGCGGGCTGGGGACTGATAAGTGCTTTAAGCAATGTCCATACCCCGTC980.11632185545229023No Hit
TGAGTAGCTGGGATTGCAGGTGCACGCCACCACACCCAGCTAATTTTGGTAT980.11632185545229023No Hit
TTCTGTGGGGATCTGAAACTAGGGGGAGCCTGGAGTATCCTTCCATTTCTCA970.1151348977435934No Hit
AACCGAGGGGTGTAGAACTGGTCATAGAGGAAGAGCTAGAAATCCAGTAGCA970.1151348977435934No Hit
CTAAAGGGGATTCTAAATAAGATATTTCTGATACTATACCATTTTCTCAGGA970.1151348977435934No Hit
TGTTCCGGGGTGAGTCTGCGACAAGGGCCCCACGGGGACGGTGCTCGGCGTC960.11394794003489657No Hit
ACAAGAGGGATGTTTATTCAGAATCTTCTGTGGGAAAAGAATGTGAGAAACA960.11394794003489657No Hit
TAGCCAGGGTCTAGGTATGGGTGTTAATTTATTTTATAATATTGAGTCATAT960.11394794003489657No Hit
GGGAAAGGGAAAATAATTCATTTTCTAAGACATTTTTGAGGAGATAATACTA960.11394794003489657No Hit
ACGTGGGTAGATATATGAAAAACAAAAAGCAGGAAATTAAATCATACCACCA960.11394794003489657No Hit
CCCCAGCCCCTCATCTTCCACATGGAGATGCTGAAGGTGGAGAGCCCTGGCA960.11394794003489657No Hit
CAGTTGGGGGACGCTCTTCCGATCTCTAGAAGGGTTATTTGGGCAAGTTTAA960.11394794003489657No Hit
TTTTGTGGGAGATGAAACAAGCCATGATTATAATTAACCTGTCAGGGCTCTT960.11394794003489657No Hit
TCATTAGGGACTACACTCATTTAAATATTTTTAAGAAAATAAAAAGCTTAAG950.11276098232619972No Hit
TGCAAGGGCATCTCTTGGCTGTACTATAAGAACACATTAATTCAATGGAAAT950.11276098232619972No Hit
TCGTTAGGGATGTTCTATAAATTCAGAGGAGTAGAAGATTACAAAATTCCAA950.11276098232619972No Hit
GCACAGGGCCGATCTGTCAAGGGTAGACTCAAATGTTGTAAAGCAGGTGCTC950.11276098232619972No Hit
GGAATAGGGTGCACAGGGGAGAGCTGTAGAGGTTACAGTAGCATCACCAGCC950.11276098232619972No Hit
AGTGTGGGGAGTGGAGAAGAGGTGAGAAAACCTTGATTACAAAACCAGCATG950.11276098232619972No Hit
CCCCCCACCACAGTCCCCAGAGTGTGATATTCCCCTTCCTGTGTCCATGTGA940.11157402461750288No Hit
AACTATGGGACGACCTTAACAACCCGGACAAGCACGCCAAGCTGGACTTCCT940.11157402461750288No Hit
CTCCCATGGTCTCTTTGATTCATCCTTTCTGACTTGGAACTTTAAGTCTGTA940.11157402461750288No Hit
AACATGGGGGGCAACAGTGGACTCAGACTTCTGAGGAATTTGGGTTTGTTCC940.11157402461750288No Hit
TTTACTGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT940.11157402461750288No Hit
GCCAAGCGGGCACTCAGGGCATTGTATCTGATAGCCACTAATGGAACTCCAG940.11157402461750288No Hit
ACAATTGGGTTGGAAATGGATATCAGACTATGAAGGTCATCAATGTTATCTG930.11038706690880604No Hit
CTGTTTGGGAACTACTTAAAGGGTGATTTTCTGTTACTTTTTAGCAAATTTT930.11038706690880604No Hit
AACCGAGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG930.11038706690880604No Hit
GTTCACGGGGTATATTGATTGGGAGAAACTTGAACGCAAAGAGATCCAGCCC930.11038706690880604No Hit
TTATGAGGTGCAGTATAGAAGGCAAATGCAATTATAGTCTTTATTATAGTAG930.11038706690880604No Hit
CGTCTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA930.11038706690880604No Hit
GCTTGGGTACAAATGTCAGCTCTGAAGAGGAGGAAGTAAATCTTCAATGCTA930.11038706690880604No Hit
TCTACAGGGAGAGGGAGCGGGCAGGGAAAGGGGGAGAGTGTTCCAGGAATGC930.11038706690880604No Hit
ATAGCAGGTGGAACATCTGAATACAACAGACCCCAACAGTACTTTCCGCTGT930.11038706690880604No Hit
TCTGTTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT930.11038706690880604No Hit
AATTAAGGGAAGAGAGATATATAAATCTCTGAATCTATTTGTGGATATGTCC930.11038706690880604No Hit
TTCCAAGGGGTGCCACCATCACCCCTGATGAGGCCCGTGTGGAAGAGTTCAA930.11038706690880604No Hit
TCGGTTGGGGATATAGGAGAATTCAGCTTATCTAGACAGTCTTAAGTTTCTA920.10920010920010921No Hit
TAAAGCAGGGGAAAGAATAAAAAGACAGCTGTCAGAACCACAAGACAAACAG920.10920010920010921No Hit
CGGACCGGGTGGTTAGAGAAATTTTCTGTCCTAAAAGTAGAAATAGCCCCTT920.10920010920010921No Hit
GTACGAGGGAAATGCCAAGGACCTTTTGCTGCCTAAATCCACTCTGCAGGAA920.10920010920010921No Hit
CAAATAGGGTCTGTGAGAGGGCAGTATTACCTGCCATGAAGAGATATTTTAT920.10920010920010921No Hit
AAATCCGGGCTAGATCTAAAGAGAGAAAAAGACATCAATACAATAATAGAAG910.10801315149141236No Hit
TTCTTAGGGCAAGGTACAGGGAGCTAGGAGAGGGTTTTACTACAGCAGAAGA910.10801315149141236No Hit
GAATGCGGGCCCTCCTCCAGGCCACGGATTTCATGTCCCTGTGACTGGTGGG910.10801315149141236No Hit
GCGCCAGGGGGCGTGTGTGCGCAGGGAGAGAACGCCGGCCACCTTCCCGCTT910.10801315149141236No Hit
AATCAGGGCGTCTGGATCCAAATGTTCAGAAATGGGATGTAACAGTATTAGA910.10801315149141236No Hit
TCTTTCTGGGGATCCGAATGAAAAGAGTAAACACAAGTTTATGGTACAGACA900.10682619378271552No Hit
GTTAACGGGATTTACCTTAAGTAGGTATAACTCTTATGGGATATACAGTATA900.10682619378271552No Hit
TGTAACGGGTGATAGAAGTAGCATGTTGCTGCCAGAATACAAGCATTGCTTT900.10682619378271552No Hit
GCAGTAGGGGAATTGAGTTTTCTTTTCTTGATGTTGGTTTCCTTCATATCAC900.10682619378271552No Hit
ACGTGGGGGGATGACTGTGGAGGGGCCACCCCCCAAAGATACTGGCATTGCT900.10682619378271552No Hit
TTAACGGGGAACGGCTCTTTCCCTCAACCTTAGTCTAGTCCCAGGGCTGAGG900.10682619378271552No Hit
AGCCTAGGGGAGTGAACAGTTCCTATCTGTATATTTGACTAAACCTTTTCCT900.10682619378271552No Hit
CACTACGGGTGAGCTGTGAAAGGGTGAGGGGAAAGGAGCCATGTCGGAGAAG890.10563923607401868No Hit
TAGGAATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTAC890.10563923607401868No Hit
GCCTTTGGGGGGAAGATATCACAAACATAATCTGTGGCTCAAAGGGGAGGTC890.10563923607401868No Hit
TTCGCAGGGATAGTTTCCAAGAATAATATAAAGGAATACAGAGATAGTTTTT890.10563923607401868No Hit
CAAAGCGGGAATAAAGTGCCATTTTGTACAGCACTCTATTTAAGTAGTATGT890.10563923607401868No Hit
GCATTCGGGTAATTCGATAAATATGTATTAAGCTGTAAATGGCAATACTTGT880.10445227836532185No Hit
AATTGTGGGGTCCTTCACACTAGCTACATGATTTATAGTCTTGCTCCCCAAT880.10445227836532185No Hit
ATGCGAGGGTCTCAAACCGGGGATCTGTACTGGGGGTTTTTCTCCCATTTTC880.10445227836532185No Hit
TCAAGAGGGACTGTACTAGGAGTTATTATTCAGATTTATTTACCCACAAAAT880.10445227836532185No Hit
CTCGCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC880.10445227836532185No Hit
TTGGACGGGTATCTGAAGTTGGGATGTGATAATTGGGTTTTTAAAAATCTGA870.10326532065662501No Hit
TTTGAGGGGATGCCAGGAAGTCATCATTTCACATGCATTAGTTGCAGAAGTT870.10326532065662501No Hit
GAAGCAGGGGGAGCTGCGCTGCTCGTTTCCAACCCTAGGTGCTGACCTCTAG870.10326532065662501No Hit
TCTGCCGGGTTGGTCAACTTACTGGTAACACCAGAACAAGAAAGCAAAAAAG870.10326532065662501No Hit
TAAAAAGGGAAAGACAGCCAGTTCGAAACTGATATAGTACAACGAATGGAAC870.10326532065662501No Hit
GCGTCCGGGATTCTTTTTCAAATCTTTGTTTCTCCACACAATTGATAGGAAT870.10326532065662501No Hit
TAGTGCGGGATGCTAAACTCACCAAATACGTTACATGTTAAATGTTTCATTC870.10326532065662501No Hit
ATTCTAGGGCAATTTGAAAATAAAAATTGAAAACAGTATGCTTTATAACAGC870.10326532065662501No Hit
TATTCCGGGAGGTACCATCCATGCATACAATTTGGTCATAGCAAAAGTAATT860.10207836294792816No Hit
ATTGCCGGGCCGATCTTTGGTAGGGATCCTCAGGTCTGCAACCAAAGATTCA860.10207836294792816No Hit
CAGCGGGGATACTTTGTGATCTAAGGTAAATCACAGTAATGAAAACTAATCT860.10207836294792816No Hit
GCAAGGGAGTCAGGGAACAGATCAGCCCGGCCCCTGCATAGCCTCAGTGTGC860.10207836294792816No Hit
GCTATAGGGGAAGATGTGCAGTAAGGTAGAAGAAAAACAAAAAGAGTCTAAT860.10207836294792816No Hit
TCCATAGGGGTTGGTGGTGGTTGTGGTGGGGAAATGTGGATGGGGGATGTCC860.10207836294792816No Hit
TGACATGGGCAAATAGCAAGAGAAGTGCATTTAGCTTCTTATGATTTGCACA860.10207836294792816No Hit
ATCTCGGGGCCTCAGTGTCAGAGGTGGAGCAGTCATACGATGACTATGAGTT860.10207836294792816No Hit
TGATCAGGGTTAAAAAAGCACCGTGGAGAATGGCCCTCTTCAGGAAAGAAAA850.10089140523923132No Hit
ATAAAAGGGACTGAAGTACTAGGTAAATGTTTTTGTTTTGTTTGTTATTTAT850.10089140523923132No Hit
TACCATAGGGGCTGGCAAGGGAGGAGGGCCGGCCAGCATTTGGTGGCCCATC850.10089140523923132No Hit
TACTATGGGAACATCTCTCCTTGATTGTCATTTCATTCTTTTGCATGTTATT850.10089140523923132No Hit
TAACGAGGGGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATA850.10089140523923132No Hit
ACCATAGGGATATAGATCAAAGCAAACTCATCTAGTTTCAAAATCAGTACTC850.10089140523923132No Hit
GTATATGGGATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCACAGT850.10089140523923132No Hit
GGACTTGGGGGACGAGGCCGTGGGTACGACCGGAAGCCGCAGCATGCTGATC850.10089140523923132No Hit
TATAGTGGGATTGACCCATCATCTGGTTTGGCATTTTTTGGACCATATACAA850.10089140523923132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)