FastQCFastQC Report
Sat 31 Dec 2022
SRR5849650.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849650.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111898
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT11871.0607875029044307RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC10630.9499722961983235No Hit
TTCGCTGGGATGTATGTATATGTGTATTTATTATTTTCTGAAGCTTTTGTGA2070.1849899015174534No Hit
ATTTAAGGGAACACACATCCTACATCTCAGGCCAGTTCATTCTTATCTAAAT1980.17694686232104237No Hit
AGACTGGGCTTTGATGACATTGTATAAGAAACAAAAAGCCAAAATGTTAGGT1890.16890382312463137No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1810.16175445495004379No Hit
CTATGAACGGGAGGTCACTTGGATATTAGATAATGAGGGAAAATGCCAGGAA1800.16086078392822034No Hit
GCGCACGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1680.150136731666339No Hit
GTAACCGGGATAGTAAGCCCTAAATTTCAGTGAACGAATGTTCTTCAAGGAA1580.1412000214481045No Hit
GGGCATGGGCTGACCAAGCAAAGAAACATTTAGTTGATGGTGCTTGATATTT1580.1412000214481045No Hit
TCCAGGGGAACCTATGAATGAGACTTTTACACCACGTAAACTTGCTCTTCCT1530.13673166633898728No Hit
TTCAGGGGGAAAGGGGGAATTTGGAGTGGAAGGCATGAGGGAAAGGTACTCA1520.13583799531716384No Hit
GTACTGGGGAAATACGATAAAGTGATAATTTCTTCATCTCTCCATCTAAGGT1520.13583799531716384No Hit
ATTGCTGGGATGTTGATTTTTGGGCAGGTATGTACTGGCAGTAAGTTGAAAA1510.1349443242953404No Hit
CCGCCGGCTAAAGCCCCGGATCCTGGCCACCCAGATCCCCTCACCTGAAGGC1510.1349443242953404No Hit
TCATCTGGGAGCAGTAAAAAGAAAAGTAGAAAATTACAGACAAGGAGAGCAA1500.13405065327351695No Hit
GCTTGTGGGAGTGGAATGAACTGTTATGGTCTCCCTGATTGATTATAATTTT1500.13405065327351695No Hit
TAAGAAGGGGGAGGCTTCTAAGCCAAATGGACAGAGGTATGATAGTGTCAAT1490.1331569822516935No Hit
CACACTGGGGAGGCCCTGCATGGGCCAGCCCCTCCATCTGTACTTGGTCCAA1430.12779495612075284No Hit
TACTGCGGGAGGCTGATTTTTAAAAATCTTTCCTAAGAAAAAAGCATGCATA1430.12779495612075284No Hit
GCATAAGGGATGTTTGGATCTCCGTAAACACATTTTTGTTCTTAGTCTATCT1430.12779495612075284No Hit
GCAACTGGGACTTCTTCTTCACTTTCACTGTTGGATTCTTCACAATCTACAA1410.12600761407710592No Hit
TTGCAGGGGAACTGACTTATTGCCCATCCTGGCAGAAACAGCCACTTAGATA1410.12600761407710592No Hit
GTATGCGGGATTCATAAGCTAATATTTTTTTAAAGTTACATTAAGATTTTTT1410.12600761407710592No Hit
ACATGCGGGCACTTCCGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTG1400.12511394305528248No Hit
TTGCTAGGGACACGAGGCTCAGCAGGCCATGGACATCCACTTCCACAGCCCG1400.12511394305528248No Hit
TTCTAAGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC1390.12422027203345903No Hit
ATTATCGGGATGTTGAGCCTTGCTTACCAGCAAAGAATATTTTTAATGTGGA1380.12332660101163559No Hit
TGTTTGGGGCATCTGTTTGAGGGAAAGGTCTCTGGGCAAGCAAGTGGTTATT1380.12332660101163559No Hit
TCGGTTGGGGGCAGTCTGTCTGAGGGCGGCCGAAGTGGCTGGCTCATTTAAG1370.12243292998981214No Hit
ATCGCTGGGATACTTAAAGTTCCATCAACTGGACTTCAATGATTCTATGTCT1370.12243292998981214No Hit
GCTGCTGGGGCGTGGGCAGCGGGCCCTGCGCCTCACTGCACCCCTCCCTGCA1350.12064558794616526No Hit
GGAATGGGGCAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGAAAGTGGC1340.11975191692434181No Hit
ATCGTAGGGGTTGTCTCCACTGTTTCCTGGAGAAAGGTCCAATTACCCAAAA1330.11885824590251837No Hit
TTTGATGGGCCTGGCTGGTGGGAATACACAGAATATATACTGAAAGGAGGCT1330.11885824590251837No Hit
ACCATGGGCAGCCTAACATACTGAGTTTTCTTTAACTTTCTAAATTATTGAA1330.11885824590251837No Hit
AAGAAGGGGTGGATAGTATTGTAAAGACAAGTCTTCTATGCTTTGAAATAGA1330.11885824590251837No Hit
ATATATGGGGCTTTGAGCCAGCCAACCAGATGGTGAAATGTGACCCTCGCCA1320.11796457488069492No Hit
GTACTAGGGGATTACCGATACAGAAGACGATCACCTTCTCCTTATTATAGTC1320.11796457488069492No Hit
CGTGCTGGGATTACAGACGTGAGCCACCGTGCCCGGCCCTTAAATATTTATG1320.11796457488069492No Hit
AGAATGGGGACAACAGTGTGGGAGAGAATCAACCGTAAAAATGTCTTCATTA1300.11617723283704803No Hit
AGGCGCGGGGCATGGGAAAATCTTCTTTACAAAAAGGAACCTGAATATGATT1300.11617723283704803No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT1300.11617723283704803No Hit
GTTTCCCCAAAGTCCGAGCGTGCAGAATTTGCTAGAACTGTCAATCTGTCTT1290.11528356181522458No Hit
CAGTAGGGGTCAAAAACAAGGTTAATTGATACCTACCATAAATTCTACCTGA1270.11349621977157769No Hit
TGTAGACGGGATAAAGACCCAATTACAGAATTTGTTGAATGTTTATATGTTA1260.11260254874975424No Hit
TCACCCAGGGATACTTGATCCCAACAAAATGATTTACATTATAAATTCACAT1260.11260254874975424No Hit
AAATATGGGAAGGGAATTTGTCAAATAATGCTGTTTAGCTAATTGTTGCAAG1260.11260254874975424No Hit
CTAAGCGTGGGCAATGCTAAAACTAATCGTCCCAACAATTATATTACTACCA1260.11260254874975424No Hit
TAAATTGGGATTTAAAGTCACGCCTTGTGTTTTTTTTACTACTTTATTCAGC1250.1117088777279308No Hit
CTATATGGGACTTGTTTTGTGGCTTGTCATGTGTAAGGCACTATGTTTAATC1250.1117088777279308No Hit
GGTCCTGGGAAGATCTAATTTTGTACTTTCCACTTCTCAGACTTGGAATTAA1250.1117088777279308No Hit
CCAGAAGGGGGGGGCATGGCAGCATTGTAAAGTGTGGCTGGTACAAACCAAA1250.1117088777279308No Hit
GTTGTTGGGCAGCACACCTGCCAGCAACTTGGGGGAATAAGCGAAGGTTTCC1240.11081520670610735No Hit
GCTATGGGGACCGAAATTTGATCAACAAGCTGTCCGTTGTGGATCAGAAGCT1240.11081520670610735No Hit
GCTTTGGGGCTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA1240.11081520670610735No Hit
ATATGAGGGACACTTCTCCTGGGAACACAAAATGGTCAAACCACTTTGGAAA1240.11081520670610735No Hit
TAGGACAGGGTCCGATCTAGCTATGGGAATCTAGCATAGTTGAACCTCTGAA1230.10992153568428391No Hit
GAATCAGGGATATGAGTAGAACTTTTATTTTAAAATGATTTTTAAAAATGCT1230.10992153568428391No Hit
TGAAAGGGAGACCACCAGATTTCTTCTGGTGAAAACACCAGATCAGGTAAAT1230.10992153568428391No Hit
CTCTAGTTGGGGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTA1230.10992153568428391No Hit
TGATACGGGATTTTTGTATTACTTAAGATAGATTATCAGAAAGACAGTTACT1220.10902786466246046No Hit
CTACACAAGGGGTGAAGTTGTGTTTTTATTTTGTTTCATTTTTTGCAGAGTA1220.10902786466246046No Hit
AAGCAGGGGACTAATGTGTTTATTGAGAGCATTTTACCTTCCAGACTTCTCA1220.10902786466246046No Hit
ATAAGCGGGCTTCATTACAGAAGGCATATCACCTTCTACTCCCTCTATAATA1220.10902786466246046No Hit
GTCTGGGGGGCAAAAGAATGATGTTGTTTCTTTGTATGTCCTTGCTTCTTTT1220.10902786466246046No Hit
TTGTAAGGGAGGGCAGGCAGCCATCGGGTTGTCTTGCATCTGTTCTGATGGG1210.10813419364063702No Hit
GGGCATGGGGAAAAAACTTGTAGATAAACTATATAGCCTAGAAATAGTGACA1210.10813419364063702No Hit
GACCTTGGGATTCATTTATCTGTTTATTCATTTATTTATTGTCTGTTCTGAG1200.10724052261881357No Hit
CGTTAGGGGAAGGCTTTCAGACAACAGTAGGCTATTAATAGTTAAGTTTGAG1200.10724052261881357No Hit
TTGTGGGAGGGCTAACTAGATAGTTACAGTAATTAAGGCAGTGTAGAGTTGT1200.10724052261881357No Hit
GAACATGGGACAGAAATTAAGACTGTGGTACTAGCATCAAGATAAACAAATA1200.10724052261881357No Hit
CACCAGGGAAGGCAGAAGGATAAAGACAAGATTTAAAAAAATGAGAAAGAAG1200.10724052261881357No Hit
GTTAAAGGGTCTGCGCACGGGATTGGGAATACTCAAGTGATACAGAAGAGAT1200.10724052261881357No Hit
TAGATGGGTCAGAGATGGTTATAGTTTTAAAAATTGAAATATTTTTAAAAAG1190.1063468515969901No Hit
TGTCAAGGGATATCATAGAAGACTCAGGCACTTGGTAACTTTCTCTTAAGTA1190.1063468515969901No Hit
CATACGGGCAAACACCCCAGCAAGAAAACTCCCAAAGTGCAGAACCAGATTC1190.1063468515969901No Hit
ATGTTGGGGGAATGGAGTCTGCACTGTGTATGGCAAAAAAATAGATGGACAG1180.10545318057516666No Hit
ACGTCCGGGTGTCAGTAAGAACATAATTAAGTTATATTTTTCTCCAATTCAA1170.10455950955334321No Hit
CCTAGCCGGGGTCACCTAATTGGGATAGTAGAGGATCACCTGTATTTGGAAT1170.10455950955334321No Hit
GTGGCAGGGTCACCCTAGATTGGCATTTTTACATCAGTTTTATATCAATCTC1170.10455950955334321No Hit
TTGCTTTGGGGATCTAATCTAGGGATGCCCATTTGTTTTTATGGCTTTCTTA1170.10455950955334321No Hit
GTAACTGGGAATTATATCTTTCTATATCCAGCCATCTGGAAATACATAGGGG1160.10366583853151977No Hit
CTGTTTGGGGCAGTTGGGGCTGCCATCGGGGGACCAAATTTGGTGTTTGTTT1160.10366583853151977No Hit
TATCGAGGGTAGAGTACACAGTTAAAATAAATATTATTTTTTCATAAGTGTT1160.10366583853151977No Hit
TCAAGTGGGATCTGCAAAAGGGGATCTATCCATGGGAGTCAATAGGTTACTA1160.10366583853151977No Hit
ACGCATGGGGGAAATGGAACGAGATATGGATAGAAAAAAGATTGAAGAATTA1160.10366583853151977No Hit
GGTAACGGGGAAATTGAATAATGGTAATTTTAAGCAGTATATCTATGAAATG1160.10366583853151977No Hit
CTTTATGGGACCATTTTGCGATGTGTTTTATTCACTTGTGTTTGTATATAAA1150.10277216750969632No Hit
TCTGTCGGGGAGAAGTGTCCTTTATATTACCAGAAAATATGGGCTTGGCCTA1130.10098482546604944No Hit
GTCAAAGGGTACACGACGCTCTTCTGATCTCATTGTGGGCAAGAACTGAAGG1130.10098482546604944No Hit
TTAGCTGGGGTTGTGCCACAGAGTGTAGCCAGGTCCCCCTGCAGCTAGTCCT1130.10098482546604944No Hit
GGGCACGGGCATACAGGTGAGAACGTGCAATAATTTGTCTTTCTGTGCCTGA1120.10009115444422599No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)