FastQCFastQC Report
Sat 31 Dec 2022
SRR5849648.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849648.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111658
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT11441.0245571298070895RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC10190.9126081427215247No Hit
AGACTGGGCTTTGATGACATTGTATAAGAAACAAAAAGCCAAAATGTTAGGT1890.16926686847337405No Hit
ATTTAAGGGAACACACATCCTACATCTCAGGCCAGTTCATTCTTATCTAAAT1790.16031094950652885No Hit
TTCGCTGGGATGTATGTATATGTGTATTTATTATTTTCTGAAGCTTTTGTGA1760.1576241738164753No Hit
CTATGAACGGGAGGTCACTTGGATATTAGATAATGAGGGAAAATGCCAGGAA1740.15583299002310627No Hit
GTAACCGGGATAGTAAGCCCTAAATTTCAGTGAACGAATGTTCTTCAAGGAA1720.15404180622973723No Hit
TCACCCAGGGATACTTGATCCCAACAAAATGATTTACATTATAAATTCACAT1670.14956384674631465No Hit
CAGTAGGGGTCAAAAACAAGGTTAATTGATACCTACCATAAATTCTACCTGA1610.14419029536620753No Hit
GTACTGGGGAAATACGATAAAGTGATAATTTCTTCATCTCTCCATCTAAGGT1570.14060792777946945No Hit
GCTTTGGGGCTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA1550.13881674398610042No Hit
TTCTAAGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC1550.13881674398610042No Hit
TAAATTGGGATTTAAAGTCACGCCTTGTGTTTTTTTTACTACTTTATTCAGC1540.13792115208941588No Hit
GAATCAGGGATATGAGTAGAACTTTTATTTTAAAATGATTTTTAAAAATGCT1530.13702556019273138No Hit
GCTTGTGGGAGTGGAATGAACTGTTATGGTCTCCCTGATTGATTATAATTTT1530.13702556019273138No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1490.1334431926059933No Hit
GCTCCGGGAGAGTCATTGTTCTTTCGAGCAGTCATTCATATCAGCGTAATGA1480.13254760070930877No Hit
ACGTCCGGGTGTCAGTAAGAACATAATTAAGTTATATTTTTCTCCAATTCAA1470.13165200881262426No Hit
TCATCTGGGAGCAGTAAAAAGAAAAGTAGAAAATTACAGACAAGGAGAGCAA1470.13165200881262426No Hit
GCAACTGGGACTTCTTCTTCACTTTCACTGTTGGATTCTTCACAATCTACAA1450.12986082501925525No Hit
TGTTTGGGGCATCTGTTTGAGGGAAAGGTCTCTGGGCAAGCAAGTGGTTATT1450.12986082501925525No Hit
TACTGCGGGAGGCTGATTTTTAAAAATCTTTCCTAAGAAAAAAGCATGCATA1440.12896523312257072No Hit
CACACTGGGGAGGCCCTGCATGGGCCAGCCCCTCCATCTGTACTTGGTCCAA1420.12717404932920168No Hit
CCGCCGGCTAAAGCCCCGGATCCTGGCCACCCAGATCCCCTCACCTGAAGGC1420.12717404932920168No Hit
TTGCAGGGGAACTGACTTATTGCCCATCCTGGCAGAAACAGCCACTTAGATA1410.12627845743251714No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT1400.1253828655358326No Hit
ACAAGTGGGGTGGCTTTAACATCCATGTGACTAGTGTATATGGTATTTGCTC1390.12448727363914812No Hit
GGGCATGGGGAAAAAACTTGTAGATAAACTATATAGCCTAGAAATAGTGACA1370.12269608984577908No Hit
CGCGTAGGGACCCAAGGGAGTGCCACGGCCCCTGACAGCCCCACAAGCTTTT1360.12180049794909456No Hit
TGTAGACGGGATAAAGACCCAATTACAGAATTTGTTGAATGTTTATATGTTA1350.12090490605241003No Hit
GTCCTTGGGATACTTGAAAAAAATTTCATTTAGAAACCTAGTTCAGAATCTG1350.12090490605241003No Hit
CTAAGCGTGGGCAATGCTAAAACTAATCGTCCCAACAATTATATTACTACCA1350.12090490605241003No Hit
GGTCCTGGGAAGATCTAATTTTGTACTTTCCACTTCTCAGACTTGGAATTAA1340.12000931415572552No Hit
ATTGCTGGGATGTTGATTTTTGGGCAGGTATGTACTGGCAGTAAGTTGAAAA1330.119113722259041No Hit
TTCAGCGGGAGCCAGCCTAGATGCGCTTAGGTGTGAAGTGCCTCACACTGGC1330.119113722259041No Hit
GCATAAGGGATGTTTGGATCTCCGTAAACACATTTTTGTTCTTAGTCTATCT1330.119113722259041No Hit
GACCTTGGGATTCATTTATCTGTTTATTCATTTATTTATTGTCTGTTCTGAG1320.1182181303623565No Hit
TTCAGGGGGAAAGGGGGAATTTGGAGTGGAAGGCATGAGGGAAAGGTACTCA1320.1182181303623565No Hit
AGGCGCGGGGCATGGGAAAATCTTCTTTACAAAAAGGAACCTGAATATGATT1320.1182181303623565No Hit
TAAGAAGGGGGAGGCTTCTAAGCCAAATGGACAGAGGTATGATAGTGTCAAT1320.1182181303623565No Hit
CGTGCTGGGATTACAGACGTGAGCCACCGTGCCCGGCCCTTAAATATTTATG1320.1182181303623565No Hit
GCGACAGGGAAAGCTTTTTAATTGAGATAATTCACATATCATAATATTCGCC1310.11732253846567196No Hit
CCAGAAGGGGGGGGCATGGCAGCATTGTAAAGTGTGGCTGGTACAAACCAAA1300.11642694656898743No Hit
CAACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.11553135467230292No Hit
TCAAGTGGGATCTGCAAAAGGGGATCTATCCATGGGAGTCAATAGGTTACTA1280.1146357627756184No Hit
GCGCACGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1280.1146357627756184No Hit
CTCTAGTTGGGGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTA1280.1146357627756184No Hit
CCATGACCGGGACACTAACTAGTTCCTTAGCTACCTTCACATACATTCTATA1270.1137401708789339No Hit
TTCAATGGGGGAAATGGAACGAGATATGGATAGAAAAAAGATTGAAGAATTA1270.1137401708789339No Hit
TATCGAGGGTAGAGTACACAGTTAAAATAAATATTATTTTTTCATAAGTGTT1250.11194898708556485No Hit
ATCCATGGGACCAAATTACAAACGCAATACTACAGCATTTGTATATTAAGTA1250.11194898708556485No Hit
GTTGTTGGGCAGCACACCTGCCAGCAACTTGGGGGAATAAGCGAAGGTTTCC1240.11105339518888034No Hit
ATTATCGGGATGTTGAGCCTTGCTTACCAGCAAAGAATATTTTTAATGTGGA1240.11105339518888034No Hit
AACAAGGGACTGGTCCATTGGCCAAAGTTTGGAGCATAATGTTCCTGGTATA1240.11105339518888034No Hit
CACAGTGGGCTTCCGATCTGATAGAGGGAGAACTGGTTAGAGGACTCCCTCA1230.11015780329219581No Hit
AGAATGGGGACAACAGTGTGGGAGAGAATCAACCGTAAAAATGTCTTCATTA1220.10926221139551129No Hit
TTGTGGGAGGGCTAACTAGATAGTTACAGTAATTAAGGCAGTGTAGAGTTGT1220.10926221139551129No Hit
AAGCAGGGGACTAATGTGTTTATTGAGAGCATTTTACCTTCCAGACTTCTCA1220.10926221139551129No Hit
TTGCTAGGGACACGAGGCTCAGCAGGCCATGGACATCCACTTCCACAGCCCG1220.10926221139551129No Hit
TCCAGGGGAACCTATGAATGAGACTTTTACACCACGTAAACTTGCTCTTCCT1220.10926221139551129No Hit
TAACTCGGGCGATCTTAATAAGGGACTACTTAGCAGCTAACAATGAAATGTT1210.10836661949882678No Hit
ATATATGGGGCTTTGAGCCAGCCAACCAGATGGTGAAATGTGACCCTCGCCA1210.10836661949882678No Hit
GTAAGTGGGGTGTTCTGTCTTTAAAATGTAAGTGAATTTTGCATTCTCTATA1200.10747102760214225No Hit
GGGCATGGGCTGACCAAGCAAAGAAACATTTAGTTGATGGTGCTTGATATTT1200.10747102760214225No Hit
AAGAAGGGGTGGATAGTATTGTAAAGACAAGTCTTCTATGCTTTGAAATAGA1200.10747102760214225No Hit
GTCTGGGGGGCAAAAGAATGATGTTGTTTCTTTGTATGTCCTTGCTTCTTTT1200.10747102760214225No Hit
TGATGGGGTCTGTTGTTTGTGGCTGTTTCCACATTACAAAGGCTAAGCTGAG1190.10657543570545774No Hit
GCTGCGCTGTAGTCCAGGTTTCCTGAGCTCGGACCCTGGTTTCAGCTGTTTC1190.10657543570545774No Hit
TGATACGGGATTTTTGTATTACTTAAGATAGATTATCAGAAAGACAGTTACT1190.10657543570545774No Hit
ACCATGGGCAGCCTAACATACTGAGTTTTCTTTAACTTTCTAAATTATTGAA1190.10657543570545774No Hit
GTATATGGGAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA1190.10657543570545774No Hit
TCGGTTGGGGGCAGTCTGTCTGAGGGCGGCCGAAGTGGCTGGCTCATTTAAG1180.10567984380877321No Hit
GTGTTAGGGGGCTGGATATGTACTGGATTTTCACTTACAGTACATTGATTTG1180.10567984380877321No Hit
CTCATTGGGATCTAAGAATTATTTTCATAGCCCACAGTCACAAAAATTTTTT1180.10567984380877321No Hit
AAGTGTGGGATTCTTAGCCATTTGCCCTTAAAACATAGAGATGGTTATTCCT1180.10567984380877321No Hit
TATCACGGGATTGCTAAGGGTAAATATACAAACAAATTCAGAATACTGTAGT1180.10567984380877321No Hit
GCTGCTGGGGCGTGGGCAGCGGGCCCTGCGCCTCACTGCACCCCTCCCTGCA1180.10567984380877321No Hit
GTTACTGGGACCTTGTGAGCAGTTGGTTACAGCGCTGTGTGATATCAAACAA1170.10478425191208869No Hit
CGTTAGGGGAAGGCTTTCAGACAACAGTAGGCTATTAATAGTTAAGTTTGAG1170.10478425191208869No Hit
CTATATGGGACTTGTTTTGTGGCTTGTCATGTGTAAGGCACTATGTTTAATC1170.10478425191208869No Hit
TCATAGGGGGCGATTTCATGATCGTGCAAACATAGAGAGTACTAACACAAAC1170.10478425191208869No Hit
CACCAGGGAAGGCAGAAGGATAAAGACAAGATTTAAAAAAATGAGAAAGAAG1170.10478425191208869No Hit
TATTGCGGGAAATGAGTTTATTAAAGCTGTCTAGAGTCACATATGGTTTTTT1170.10478425191208869No Hit
AGTTTAGGGCTGATAAAGTCCCTGGATGTAAGAAAGAAGATGAAATGTCTTT1170.10478425191208869No Hit
ATAGATGGGCTTGCTCACAGGGTTCTTGCCATAAAGTAGACAAGTTATGTCT1160.10388866001540419No Hit
AGAAAGGGGGTGTGAAGTTTCTATTTTTAATCAAATTTAAGATCATTTCTGA1160.10388866001540419No Hit
TCAGTGGGGTGCAAACTAGCTCCTTTCTCCCACACTGGGAACTTTAGTCCAC1160.10388866001540419No Hit
ATAAGCGGGCTTCATTACAGAAGGCATATCACCTTCTACTCCCTCTATAATA1150.10299306811871965No Hit
GTATGCGGGATTCATAAGCTAATATTTTTTTAAAGTTACATTAAGATTTTTT1150.10299306811871965No Hit
TTGCTTTGGGGATCTAATCTAGGGATGCCCATTTGTTTTTATGGCTTTCTTA1150.10299306811871965No Hit
ATGTTGGGGGAATGGAGTCTGCACTGTGTATGGCAAAAAAATAGATGGACAG1140.10209747622203515No Hit
CATTGAGGGACGACATGTATAGGTTACCTAGATGTTATGAGAAATTTTAGTT1140.10209747622203515No Hit
CAGCCTGGATCTTATTGTCCATAGCATTATCAGCATTTTGGTCAAAGCCATT1140.10209747622203515No Hit
TAAACCGGGAGCCAGTATCAAGCTCTCAGTCAAGATCCAAACAGTCTTTCAA1140.10209747622203515No Hit
TTCTAAGGGATAGTAGTGCATGCGAACAGTAACAGAAACCAGGAAGAATACA1130.10120188432535063No Hit
TTAGCTGGGGTTGTGCCACAGAGTGTAGCCAGGTCCCCCTGCAGCTAGTCCT1130.10120188432535063No Hit
GGAATGGGGCAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGAAAGTGGC1130.10120188432535063No Hit
GTGGCAGGGTCACCCTAGATTGGCATTTTTACATCAGTTTTATATCAATCTC1130.10120188432535063No Hit
ATCGCTGGGATACTTAAAGTTCCATCAACTGGACTTCAATGATTCTATGTCT1130.10120188432535063No Hit
TAAATTGGGACACCCTGTTATGTTCTCCAGAAACCTAGCAGGTTGCTTTACA1120.1003062924286661No Hit
GGGCACGGGCATACAGGTGAGAACGTGCAATAATTTGTCTTTCTGTGCCTGA1120.1003062924286661No Hit
TGTAGGGTCACTTACTCCATGAATATTTTATAGGTACCTGTTATGTCTCCAA1120.1003062924286661No Hit
TATATAGGGGACGAGCCCATCCTCAGCAACCGATCTGGAGACCACCGTGGCA1120.1003062924286661No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)