FastQCFastQC Report
Sat 31 Dec 2022
SRR5849641.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849641.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100675
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC10531.0459399056369505No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10261.0191209336975415RNA PCR Primer, Index 27 (95% over 22bp)
CATAGAAGGGGACGCTCTTCCGATCTTAAGTGGGAATCCTCACCACGGCCAT2410.23938415694065063No Hit
AAAATGGGGGATCTTAGTGAGGGAATGTATTTAATGTTTAACTTTGTTTACC2170.21554507077228705No Hit
GCTGCTGGGATAGCTCAGGCATTTGAAAGGATGATGAGTCCAACTGGTTCTA2030.20163893717407502No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1720.17084678420660543No Hit
GGATTGGGATAGAATTTATCATTTCTGAGTACCTTTTTTATTCTTAGACTTT1610.1599205363794388No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCT1580.15694065060839335No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT1580.15694065060839335No Hit
TGTGCAGGGATAATAAAAGATCAAAAGATATATCTCCTATGAACAGATTGGA1570.1559473553513782No Hit
TTTCTTGGGATGGTGTATCAGATAGGTGATAAGTACTTTGGAAAATAAGCCA1520.15098087906630245No Hit
ATTTTTGGGTGCTTGAAGAAATGAGGAATCCGGACTCCTGCTACCAAAACCA1510.1499875838092873No Hit
CAAGAGGGGGACTATATTTTACAATTCTTGAAAATGAAAGAAAATGAAAAAG1510.1499875838092873No Hit
CTGCATGGGATTGAAATCTCTTTACAGAACAGAACTAAAAAGACTTCTTTGC1460.14502110752421157No Hit
TCAATGGGGAGCTGTTTTTCGTCTTCCCTAGGCTGTTTCTGCTGGGCGCTCC1440.14303451701018127No Hit
CTATGGGGTACAACTCAATGGCATTCCATCCCAAGGGATGGTTTAATGGCAT1410.14005463123913584No Hit
GATGGCGGGGTTGAATGAGTTACTGAATGAATGTTGGAAAGTGTGTAGAATC1400.1390613359821207No Hit
CATGGCGGGATCAAATTTCCATTGAAAAACTGAAGAGCAAACTAAGATGTAA1390.13806804072510553No Hit
TCGTATGGGAAGCTCTAAGAATCTGCCCCCTCCTGAAGGGCAGGTAGAGAAG1380.1370747454680904No Hit
TTCTCGGGATTTTTGCCTAATATTAAAATCTGGCCCCAGCTTTCTTTCTGTT1370.13608145021107523No Hit
TTGCCTGGGATTATGGCTGAGTTGAAGACTTAAGGTGTAATTTCATTACAGT1370.13608145021107523No Hit
AGCACTGGGGTAGCAAATGCCATTTAACAAGATTTCTAGGCACTGCTTATTT1350.13409485969704493No Hit
TATTGTGGGCGATCTCAAGTGGGGGAGCTAGGCCAAATTGCCATTTTTGTTT1330.13210826918301466No Hit
TTCCGAGGGAGGACCACTGTGTTATCAAATGCCAGTTGTAGATTTGGATTGA1290.12813508815495406No Hit
GTGGTTGGGTTACTATAATTCATTAATATGTAGCATTGAAATATTTTTATTC1290.12813508815495406No Hit
TAGCTGGGAATAGCAATGCAGGAAAAAGGCAACCTTGTTTGTGGAAGGTTTT1280.1271417928979389No Hit
GGCGCAGGGACCAGAATAGTGGCTCTGAACCATGGCTGCATATTAGAATCAC1270.12614849764092378No Hit
CTCGCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGC1260.12515520238390862No Hit
GTATTAGGGACCAAAAATCACTTGCATTTCTATATACTATTAATGAACATAT1260.12515520238390862No Hit
ATACTGGGGAGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTC1250.12416190712689348No Hit
AGCACAGGGGTTAGGTTATGGGATGTAGACCGAGGGATATGCATCCATACCT1250.12416190712689348No Hit
TGATAGTGGGGTTGCAGCATTACTGGGTTTGGGGTGCCCCATAATGCAGATA1240.12316861186987833No Hit
GAAGTAGGGACATTTGAGGACAGGAATATGTTGAGCCTGATTTTCCTGGTGT1240.12316861186987833No Hit
CAGACAGGGGCTGCCAGTCAAGGCCTGCATATGCAGAATGACGATGCCTGCC1230.12217531661286318No Hit
CAACACGGGATTCCAAAATTGACCACATACTTGGAAGTAAAGCTCTCCTCAG1230.12217531661286318No Hit
ATAGTCGGGAGCTTATGGGAGCCTGAATTCCAGAAACTTGATTTTACTTTTT1220.12118202135584803No Hit
ATAACAGGGCTCTGCATTCTTTATATTTTGGAGATTGTTTTTCCAATCTCCA1220.12118202135584803No Hit
AGCCTCGGGGATCATCATGCTTCCGGACGAGACCACTGACTTGAGAACGGTG1210.12018872609883288No Hit
TCTAGAGGGGATTTGAAGGAGAAGGAAATACCAGAATTAAGAAACAAACTGC1210.12018872609883288No Hit
TCATTGTGGGGTCTTTTGGAGAATCTAAGTTTGCATCATTTTAAAAATGGCT1200.11919543084181773No Hit
TAGGCTGGGAACATGAACTAACATTTCTGCACTGTAACGTGCCGGGCACACA1200.11919543084181773No Hit
ATTTTAGGGCGCTCCACTGTACTCCAGCCTGGGTGACAGCGTGAGACTCCAT1200.11919543084181773No Hit
CTCAGCGGCTAGGCATATAATAATAGTTTAGAGAATGTAGACCAGATAAATT1190.11820213558480258No Hit
TCATCGGGGTCGGGATGATGCGGACGCAGTGTCTGCTGGGGCTGCGCACGTT1190.11820213558480258No Hit
ATTCTTGGGATATTAGCATACATTTCAAACTAAATGTTTTACAGTGGCTTAT1190.11820213558480258No Hit
TACAGTGGGATCAGGGAAGCAGATATTGATGGTGATGGTCAAGTAAACTATG1190.11820213558480258No Hit
AAACATGGGGAATCTGCTCCCCACCTGGATGCTGCTAGTCCTTTCTCCAGGC1180.11720884032778743No Hit
GTAAGCGGGCTCTTCCGATCTCCAAAAGGGGGAGTTTTTTTCTCTGGGAAAA1180.11720884032778743No Hit
CATCACGGGATAGTTTTAATTCTACAAATGATTCTAGTGGTCCTGCAAATCT1180.11720884032778743No Hit
GGCAATGGGAGTTTTCTTTTTTATTTTGGTTATGCTTAAATATGAATGTAAT1170.1162155450707723No Hit
GGCTATGGGAAATATCTTAAATAAAAGCCAACTTAAGAAGAGCCCAGCATTA1170.1162155450707723No Hit
CTAAACGGGGCTCCCGGCGCGGCAGCTGTCTGGGCTGCTGCGCGCCGCCTAG1170.1162155450707723No Hit
TGCGGGGGGGCATGAATCTGCTAAATCTCATTATTTCTCCTTAATATGTTAC1160.11522224981375714No Hit
AAGTAAGGGTAACGAGGAAAGACTGGGGTAATTATAAACAAATATGTTAACC1130.11224236404271169No Hit
ACTATTGGGATTGCGAGGGAAGAAATACCTCACTTTGGCCTTGTTGGGGGCA1130.11224236404271169No Hit
CACACTGGGACTCCTCGCTGCAGGGCACTGTCCCACCGGGATCCAGTTGCAA1130.11224236404271169No Hit
CAAGCAGGGGCAAGTGTAAAACACTTCTGTTATAAAAGCAGTGTTTGGAAAA1130.11224236404271169No Hit
ACTGCAGGGAATAACATAGACTGACTCTATACTTACTTGCCTCAATAACATT1120.11124906878569654No Hit
TAGAGAGGGGATTAGAGGTTTGGGCATATAACCAGATTAAAGTGAAGGAACT1120.11124906878569654No Hit
GGGAATGGGGAGAGGCCTGCAGGAGAAACACACTCCCCTCCTGGGTTTCCAC1120.11124906878569654No Hit
AAACCTGGGCGACGCTCTTCCGATCTTACGCTGGGGTAAAAAATAAAAAAGG1120.11124906878569654No Hit
AGCCTGGGGACACTCTCCAATTGTAAGAGAAAGGGGGCAGGGAAGCAATATA1120.11124906878569654No Hit
TTGCCCGGGAAGCATAAGGAAAAAAGCAAACATCAGAAAGATTTCCACTTAG1110.11025577352868139No Hit
TACCTTGGGATACCTTGCTTGCTTCCCTGATTCATTTTTTTGTTTTGTATCA1110.11025577352868139No Hit
ATTCGAGGGTGCTAAGGACAGTTAGTATTTTGCTGTTTTCGGCCCCATCTTC1110.11025577352868139No Hit
ACCCATGGGATCCATTTGCCGTCAGTACTCTAATGGCAACTGCTATTCATGT1110.11025577352868139No Hit
TCTACGGGAGTAATCTAATAGAAAGATATTTCTTTACTAAGGTAGGGGGAAT1110.11025577352868139No Hit
TCAATTGGGGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGA1110.11025577352868139No Hit
ATAACAGGGATAGTAAATCTGTTCACCTATCTGCTATGCTGGCAAATTCTGG1110.11025577352868139No Hit
TGCCCAGGGATGAAGTTCATTGTGTAGAAGGATTTATTATGACCCCCTTTGT1100.10926247827166624No Hit
AATACTGGGACAGGTAAATTGAGAGTGAAAGGAGTAAAGAAAAAATACAGAT1100.10926247827166624No Hit
ACGCGGGGTGAACAATCTTATTTTTAACTAACACTATTTTCATACACGTATG1090.1082691830146511No Hit
ACTTGCGGGAGTCAAATACAATTTATACGTACACACACACACACACACACAC1090.1082691830146511No Hit
TACCGAGGGATAGCCAATCAAAACATTGCTTTGGTTGGTGCATTTAAGTATC1090.1082691830146511No Hit
CTAAGAAGGGGAGCATCTCCAATCTGAAAATCCTTAAAGGTCCAAAATGAAA1080.10727588775763595No Hit
GTAGGCGGGGAGAGAGCTGGGGAAAGTAGCAGCAACTAGATTAAGACTGGGA1080.10727588775763595No Hit
GTACAGGGGCTTTTAAAAAAAGTCAACTAGAATTGAGAAGAGGTGATTTTTT1080.10727588775763595No Hit
ACTATAGGGATTCAAGTAAGCTCTTCCTCCCGTTTAGTTAGATAAGCCTCTT1070.1062825925006208No Hit
ACAAATGGGAAATCAAGAGGTTCCAATCTTCGTGTTCACTTTAAGAACACTC1060.10528929724360565No Hit
TTGTGTGGGACTTACTACCAAGGAACCTAAATTGGACTTTTTGAATAAAAAG1060.10528929724360565No Hit
CTAATTGGGATTCCTTTAATCTTAATGATACACTTTTTTTTCTTATAAATGT1050.1042960019865905No Hit
TGTAGAGGGCTACTGTATTTGTTTCTTTGTTTATATTTTCAGTAAGCCCTGG1050.1042960019865905No Hit
TTGCCAGGGGTGTCAATGTAGATTTATCAATTTTAGTAAACATACAACTTTG1050.1042960019865905No Hit
TCGTACGGGAAGCTGTGACATTGTCTATAAGCATTTGGCTCCACAAATGTAT1050.1042960019865905No Hit
TATTGTGGGGTTTTATTCTTTATTGTGTCTTCTTGAGTCATCTTTTGCCACC1050.1042960019865905No Hit
TACATAAGGGCGACGCTCTTCCGATCTTCAACCGGGAACTGGACCAGCACTT1040.10330270672957538No Hit
AATCGCGGGATTCACAGCTACGTGTGCCCCTCTCATGGTCATGGGTCTGCCC1040.10330270672957538No Hit
TCGCCAGGGATTCCTTGTTTTCCTGCAGTGTAATGGCCCTGAATGTCCTCTG1040.10330270672957538No Hit
CTCTTTGGAGAGAATTGATTAGAGGAATTTGGAAAGACGTTAGAAAATATGA1030.10230941147256023No Hit
CTCCGCGGGCAAGACAAAATTTTGATTAGCTTCTTGGTCAACTTCCAGTACC1030.10230941147256023No Hit
AGATACGGGTCTTCCGATCGCCTCGTGGGGAGCCTATTAAACCTCTTTTTCT1030.10230941147256023No Hit
TAGAATGGGGTGGGAAAACTTCCAGCCCTCTGTTAATTGTGTGTCTCAAATT1030.10230941147256023No Hit
TAATAGGGACCTGCTGTTTGTTTTGTTCTTAACTTCATTGTATTATGGGGCT1030.10230941147256023No Hit
GGGTATGGGAGAATTGTAGATATATAATGCTCAGACTACTTTTAAAAATCCT1030.10230941147256023No Hit
GCAATAGGGACCACGCAATGTGCAAGCAAGGGCAGGAGGTAGGTCCAATCTG1020.10131611621554508No Hit
ATTTTGGGGGTGCTTAGTCGGAATACAAATTTCACAGTGGATTTTTGAAGTT1020.10131611621554508No Hit
AAACTCCGGGGTCCAGTAAAAAACTCCTGCACTGAAGTCATTGTGACTTGAG1020.10131611621554508No Hit
AGCTAAGGGATTGACAGGCATGTTTCCCATGCATAGAATTCTCAGCTGACAG1010.10032282095852993No Hit
AGACTAGGGAGGCCAAAAAAAATGGATAATGCATCAAGAACCTGTAGGACTA1010.10032282095852993No Hit
TCTGCCGGGACAGCACTCTATTTAAGTAGTATGTTTCAGTCATACTCCTATT1010.10032282095852993No Hit
TTGGTCGGGGTGAGGTATAAGTTGAAGTCTACTTTTTTCCATATGGATGTTG1010.10032282095852993No Hit
CATCCCGGGACTGAAATCCCACCTTATGTGATGAAGTGTCCGAGCAATGGTT1010.10032282095852993No Hit
TTAGCGGGGAGAGGAGGCGCCCCGTAGGCAGCTGAGGGTATCGCAGAGAACG1010.10032282095852993No Hit
CGTTGGGGGATAACACATTATTATAGCTTGATTTTAATTTTTTGCATTAATT1010.10032282095852993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)