FastQCFastQC Report
Sat 31 Dec 2022
SRR5849640.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849640.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100428
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC10751.0704186083562353No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10691.064444178914247RNA PCR Primer, Index 27 (95% over 22bp)
AAAATGGGGGATCTTAGTGAGGGAATGTATTTAATGTTTAACTTTGTTTACC2460.24495160712151987No Hit
GCTGCTGGGATAGCTCAGGCATTTGAAAGGATGATGAGTCCAACTGGTTCTA2310.23001553351654916No Hit
CATAGAAGGGGACGCTCTTCCGATCTTAAGTGGGAATCCTCACCACGGCCAT2200.21906241287290396No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1950.19416895686461944No Hit
TCAATGGGGAGCTGTTTTTCGTCTTCCCTAGGCTGTTTCTGCTGGGCGCTCC1690.1682797626160035No Hit
GATGGCGGGGTTGAATGAGTTACTGAATGAATGTTGGAAAGTGTGTAGAATC1610.16031385669335244No Hit
AGACTAGGGAGGCCAAAAAAAATGGATAATGCATCAAGAACCTGTAGGACTA1600.15931811845302107No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT1580.1573266419723583No Hit
TGATAGTGGGGTTGCAGCATTACTGGGTTTGGGGTGCCCCATAATGCAGATA1550.15433942725136415No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCT1530.1523479507707014No Hit
AATTGAGGGTCTGTGGATGGGGGAGACCAGTGAGGCCACGGAGATCATCGAG1520.15135221253037No Hit
ATAGTCGGGAGCTTATGGGAGCCTGAATTCCAGAAACTTGATTTTACTTTTT1500.14936073604970726No Hit
ATTTTTGGGTGCTTGAAGAAATGAGGAATCCGGACTCCTGCTACCAAAACCA1500.14936073604970726No Hit
CATGGCGGGATCAAATTTCCATTGAAAAACTGAAGAGCAAACTAAGATGTAA1440.14338630660771895No Hit
TATTGTGGGCGATCTCAAGTGGGGGAGCTAGGCCAAATTGCCATTTTTGTTT1440.14338630660771895No Hit
TATTGTGGGGTTTTATTCTTTATTGTGTCTTCTTGAGTCATCTTTTGCCACC1440.14338630660771895No Hit
CTTGCAGGGAAGACTCCATCATAAATAAAATAAATAAATAAATAAATCAGAA1420.1413948301270562No Hit
GGATTGGGATAGAATTTATCATTTCTGAGTACCTTTTTTATTCTTAGACTTT1390.13840761540606206No Hit
ACGCGGGGTGAACAATCTTATTTTTAACTAACACTATTTTCATACACGTATG1370.1364161389253993No Hit
CTATGGGGTACAACTCAATGGCATTCCATCCCAAGGGATGGTTTAATGGCAT1360.1354204006850679No Hit
CAAGAGGGGGACTATATTTTACAATTCTTGAAAATGAAAGAAAATGAAAAAG1350.13442466244473653No Hit
ACCCATGGGATCCATTTGCCGTCAGTACTCTAATGGCAACTGCTATTCATGT1340.13342892420440514No Hit
GGCGCAGGGACCAGAATAGTGGCTCTGAACCATGGCTGCATATTAGAATCAC1330.13243318596407377No Hit
TTTCTTGGGATGGTGTATCAGATAGGTGATAAGTACTTTGGAAAATAAGCCA1320.13143744772374238No Hit
TAGAGAGGGGATTAGAGGTTTGGGCATATAACCAGATTAAAGTGAAGGAACT1320.13143744772374238No Hit
TCTACGGGAGTAATCTAATAGAAAGATATTTCTTTACTAAGGTAGGGGGAAT1320.13143744772374238No Hit
TGCGGGGGGGCATGAATCTGCTAAATCTCATTATTTCTCCTTAATATGTTAC1310.130441709483411No Hit
GGGAATGGGGAGAGGCCTGCAGGAGAAACACACTCCCCTCCTGGGTTTCCAC1310.130441709483411No Hit
GTGCATGGGAACCCAATTAAATGATAGAAGCCATGAGGCTTGACTGAGTAAG1290.12845023300274824No Hit
AGCTAAGGGATTGACAGGCATGTTTCCCATGCATAGAATTCTCAGCTGACAG1270.12645875652208546No Hit
TTCCGAGGGAGGACCACTGTGTTATCAAATGCCAGTTGTAGATTTGGATTGA1270.12645875652208546No Hit
CAACACGGGATTCCAAAATTGACCACATACTTGGAAGTAAAGCTCTCCTCAG1270.12645875652208546No Hit
CTAAACGGGGCTCCCGGCGCGGCAGCTGTCTGGGCTGCTGCGCGCCGCCTAG1270.12645875652208546No Hit
TAGGCTGGGAACATGAACTAACATTTCTGCACTGTAACGTGCCGGGCACACA1260.1254630182817541No Hit
TGTGCAGGGATAATAAAAGATCAAAAGATATATCTCCTATGAACAGATTGGA1260.1254630182817541No Hit
ATACTGGGGAGGCTGTTCCTTGTACTCTTAGGATATTTGAGTCACTTGTGTC1260.1254630182817541No Hit
GTATTAGGGACCAAAAATCACTTGCATTTCTATATACTATTAATGAACATAT1250.12446728004142271No Hit
CTCGCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGC1230.12247580356075993No Hit
GTGGTTGGGTTACTATAATTCATTAATATGTAGCATTGAAATATTTTTATTC1230.12247580356075993No Hit
GGCTATGGGAAATATCTTAAATAAAAGCCAACTTAAGAAGAGCCCAGCATTA1230.12247580356075993No Hit
AGCACTGGGGTAGCAAATGCCATTTAACAAGATTTCTAGGCACTGCTTATTT1220.12148006532042856No Hit
CTGCATGGGATTGAAATCTCTTTACAGAACAGAACTAAAAAGACTTCTTTGC1220.12148006532042856No Hit
AAGTAAGGGTAACGAGGAAAGACTGGGGTAATTATAAACAAATATGTTAACC1210.12048432708009718No Hit
GTAAGCGGGCTCTTCCGATCTCCAAAAGGGGGAGTTTTTTTCTCTGGGAAAA1210.12048432708009718No Hit
AGCCTGGGGACACTCTCCAATTGTAAGAGAAAGGGGGCAGGGAAGCAATATA1210.12048432708009718No Hit
TTGCCAGGGGTGTCAATGTAGATTTATCAATTTTAGTAAACATACAACTTTG1200.1194885888397658No Hit
TCGTACGGGAAGCTGTGACATTGTCTATAAGCATTTGGCTCCACAAATGTAT1200.1194885888397658No Hit
GGCAATGGGAGTTTTCTTTTTTATTTTGGTTATGCTTAAATATGAATGTAAT1180.11749711235910304No Hit
TCATTGTGGGGTCTTTTGGAGAATCTAAGTTTGCATCATTTTAAAAATGGCT1170.11650137411877165No Hit
TTGCCCGGGAAGCATAAGGAAAAAAGCAAACATCAGAAAGATTTCCACTTAG1160.11550563587844027No Hit
CACACTGGGACTCCTCGCTGCAGGGCACTGTCCCACCGGGATCCAGTTGCAA1160.11550563587844027No Hit
TCTAGAGGGGATTTGAAGGAGAAGGAAATACCAGAATTAAGAAACAAACTGC1160.11550563587844027No Hit
TTCTCGGGATTTTTGCCTAATATTAAAATCTGGCCCCAGCTTTCTTTCTGTT1150.1145098976381089No Hit
GCACCAGGGGGTAGAAGCAATCTTGACCTTTTTTATTTTTGCAGGCCCCTGC1140.11351415939777751No Hit
CTCAGCGGCTAGGCATATAATAATAGTTTAGAGAATGTAGACCAGATAAATT1130.11251842115744613No Hit
ATTTTAGGGCGCTCCACTGTACTCCAGCCTGGGTGACAGCGTGAGACTCCAT1130.11251842115744613No Hit
CAGACAGGGGCTGCCAGTCAAGGCCTGCATATGCAGAATGACGATGCCTGCC1120.11152268291711476No Hit
TAATAGGGACCTGCTGTTTGTTTTGTTCTTAACTTCATTGTATTATGGGGCT1120.11152268291711476No Hit
ATAACAGGGATAGTAAATCTGTTCACCTATCTGCTATGCTGGCAAATTCTGG1120.11152268291711476No Hit
AGCATTGGGTACGCCGGGGCAAATGATGAAAAACAAAACTGTATGTCCTTTG1120.11152268291711476No Hit
GGGTATGGGAGAATTGTAGATATATAATGCTCAGACTACTTTTAAAAATCCT1120.11152268291711476No Hit
AATACTGGGACAGGTAAATTGAGAGTGAAAGGAGTAAAGAAAAAATACAGAT1110.11052694467678338No Hit
TTCCAAGGGAACATTAGGAGTGTAGCTCTTCATAATGAAATATCTTCTTTCT1110.11052694467678338No Hit
TTGTGTGGGACTTACTACCAAGGAACCTAAATTGGACTTTTTGAATAAAAAG1110.11052694467678338No Hit
TCTAGCGGGACTTTTAAATAGGACCTTCTCTGGAAAATTATTTTGGTTAATG1090.1085354681961206No Hit
CTTATTGGGAGTGTCAGGAGGGCTTGATTTACATCTGGTCCGTGATGGTTTT1080.10753972995578923No Hit
TATCCAGGGATGTTTTTAAATACAGAAATTGCTTTCACAAATTTGTACCACA1080.10753972995578923No Hit
CTAGAAGGGTAAATGATAAAAGTAAGTCTTGCTCATTGAAGAAAAATAGAGA1080.10753972995578923No Hit
GGTTATGGGGAAGGACAACCTCAACCAATGAATGCATATTTTGGCATTTGCT1080.10753972995578923No Hit
ATTCGAGGGTGCTAAGGACAGTTAGTATTTTGCTGTTTTCGGCCCCATCTTC1070.10654399171545785No Hit
TTGCCTGGGATTATGGCTGAGTTGAAGACTTAAGGTGTAATTTCATTACAGT1070.10654399171545785No Hit
TCATCGGGGTCGGGATGATGCGGACGCAGTGTCTGCTGGGGCTGCGCACGTT1070.10654399171545785No Hit
AAACCTGGGCGACGCTCTTCCGATCTTACGCTGGGGTAAAAAATAAAAAAGG1070.10654399171545785No Hit
TGCCCAGGGATGAAGTTCATTGTGTAGAAGGATTTATTATGACCCCCTTTGT1060.10554825347512645No Hit
TACAATGGGGTCCTACAGCTTACATTCTGGTAATATTTGTCAGAAAGCAGTC1050.10455251523479507No Hit
GATGGTGGGAAAATAAAATTTTAATAAGAACGTGTTATGTGACCGTTATTTC1050.10455251523479507No Hit
TCGCCAGGGATTCCTTGTTTTCCTGCAGTGTAATGGCCCTGAATGTCCTCTG1050.10455251523479507No Hit
TTTGTCGGGACATATATTTATGAGAATATTTAGTCAAAGGAAACAAGTATAA1050.10455251523479507No Hit
TCGTATGGGAAGCTCTAAGAATCTGCCCCCTCCTGAAGGGCAGGTAGAGAAG1040.1035567769944637No Hit
TAGCTGGGAATAGCAATGCAGGAAAAAGGCAACCTTGTTTGTGGAAGGTTTT1040.1035567769944637No Hit
AGATACGGGTCTTCCGATCGCCTCGTGGGGAGCCTATTAAACCTCTTTTTCT1040.1035567769944637No Hit
TGACCAGGGACAAGAAAACGCATTCTCAAATTCATATCCACTTCAAACTCTC1040.1035567769944637No Hit
TCTGCAGGGGAAACATTCCATGTTTTAATCTGAGCCTTGCAGACTTTCATTT1030.10256103875413232No Hit
TACATAAGGGCGACGCTCTTCCGATCTTCAACCGGGAACTGGACCAGCACTT1030.10256103875413232No Hit
ATTTTGGGGGTGCTTAGTCGGAATACAAATTTCACAGTGGATTTTTGAAGTT1030.10256103875413232No Hit
AAACTCCGGGGTCCAGTAAAAAACTCCTGCACTGAAGTCATTGTGACTTGAG1030.10256103875413232No Hit
TATTATGGGATTCCCCTTCCTGTGTCCATGTGATCTCATTGTTCAATTCCCA1020.10156530051380094No Hit
CATCACGGGATAGTTTTAATTCTACAAATGATTCTAGTGGTCCTGCAAATCT1020.10156530051380094No Hit
ACTCATGGGAACTAAGTACTATGATATACAAGTAATGACATGAATACGTGGG1020.10156530051380094No Hit
TTAGGACGGGATGAGACAACTAACATGAAGTGAAAGGTTGCATTTGTTTTCA1010.10056956227346954No Hit
TGCACCGGGGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATTGC1010.10056956227346954No Hit
ATCCAAGGGGTAACAAACCTGCATGTTCTGCAAGTGTACCCCAGAACTTAAA1010.10056956227346954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)