FastQCFastQC Report
Sat 31 Dec 2022
SRR5849639.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849639.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102356
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC10431.0189925358552503No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT9800.9574426511391614RNA PCR Primer, Index 27 (95% over 22bp)
AAAATGGGGGATCTTAGTGAGGGAATGTATTTAATGTTTAACTTTGTTTACC2440.23838368048770958No Hit
GCTGCTGGGATAGCTCAGGCATTTGAAAGGATGATGAGTCCAACTGGTTCTA2310.22568291062565948No Hit
CATAGAAGGGGACGCTCTTCCGATCTTAAGTGGGAATCCTCACCACGGCCAT2060.20125835319864002No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1710.16706397280081284No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCT1680.16413302590957052No Hit
GGATTGGGATAGAATTTATCATTTCTGAGTACCTTTTTTATTCTTAGACTTT1660.16217906131540896No Hit
GATGGCGGGGTTGAATGAGTTACTGAATGAATGTTGGAAAGTGTGTAGAATC1530.14947829145335886No Hit
TTGCCTGGGATTATGGCTGAGTTGAAGACTTAAGGTGTAATTTCATTACAGT1520.1485013091562781No Hit
CATCACGGGATAGTTTTAATTCTACAAATGATTCTAGTGGTCCTGCAAATCT1520.1485013091562781No Hit
TTCCGAGGGAGGACCACTGTGTTATCAAATGCCAGTTGTAGATTTGGATTGA1500.14654734456211654No Hit
TTTCTTGGGATGGTGTATCAGATAGGTGATAAGTACTTTGGAAAATAAGCCA1500.14654734456211654No Hit
TTCTCGGGATTTTTGCCTAATATTAAAATCTGGCCCCAGCTTTCTTTCTGTT1480.14459337996795496No Hit
TGATAGTGGGGTTGCAGCATTACTGGGTTTGGGGTGCCCCATAATGCAGATA1470.1436163976708742No Hit
GTATTAGGGACCAAAAATCACTTGCATTTCTATATACTATTAATGAACATAT1460.14263941537379343No Hit
ATTTTTGGGTGCTTGAAGAAATGAGGAATCCGGACTCCTGCTACCAAAACCA1450.14166243307671267No Hit
CATGGCGGGATCAAATTTCCATTGAAAAACTGAAGAGCAAACTAAGATGTAA1400.13677752159130876No Hit
AGCACTGGGGTAGCAAATGCCATTTAACAAGATTTCTAGGCACTGCTTATTT1350.13189261010590486No Hit
TATTGTGGGCGATCTCAAGTGGGGGAGCTAGGCCAAATTGCCATTTTTGTTT1350.13189261010590486No Hit
TCAATGGGGAGCTGTTTTTCGTCTTCCCTAGGCTGTTTCTGCTGGGCGCTCC1330.12993864551174333No Hit
AGACTAGGGAGGCCAAAAAAAATGGATAATGCATCAAGAACCTGTAGGACTA1320.12896166321466257No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT1320.12896166321466257No Hit
TGTAGAGGGCTACTGTATTTGTTTCTTTGTTTATATTTTCAGTAAGCCCTGG1310.12798468091758178No Hit
ATAACAGGGATAGTAAATCTGTTCACCTATCTGCTATGCTGGCAAATTCTGG1310.12798468091758178No Hit
ATTCTTGGGATATTAGCATACATTTCAAACTAAATGTTTTACAGTGGCTTAT1300.127007698620501No Hit
GGGAATGGGGAGAGGCCTGCAGGAGAAACACACTCCCCTCCTGGGTTTCCAC1300.127007698620501No Hit
CTGCATGGGATTGAAATCTCTTTACAGAACAGAACTAAAAAGACTTCTTTGC1290.12603071632342022No Hit
AATACTGGGACAGGTAAATTGAGAGTGAAAGGAGTAAAGAAAAAATACAGAT1280.12505373402633943No Hit
CAAGAGGGGGACTATATTTTACAATTCTTGAAAATGAAAGAAAATGAAAAAG1280.12505373402633943No Hit
TATTGTGGGGTTTTATTCTTTATTGTGTCTTCTTGAGTCATCTTTTGCCACC1280.12505373402633943No Hit
GGCGCAGGGACCAGAATAGTGGCTCTGAACCATGGCTGCATATTAGAATCAC1280.12505373402633943No Hit
GGCTATGGGAAATATCTTAAATAAAAGCCAACTTAAGAAGAGCCCAGCATTA1280.12505373402633943No Hit
CTCGCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGC1270.12407675172925867No Hit
ACTAAGGGAGCCAAGGCATCTTTAAACAAAATGAATAAACCTGGAAGCCTCA1260.12309976943217789No Hit
GTAAGCGGGCTCTTCCGATCTCCAAAAGGGGGAGTTTTTTTCTCTGGGAAAA1260.12309976943217789No Hit
TCTACGGGAGTAATCTAATAGAAAGATATTTCTTTACTAAGGTAGGGGGAAT1260.12309976943217789No Hit
TAGAGAGGGGATTAGAGGTTTGGGCATATAACCAGATTAAAGTGAAGGAACT1250.12212278713509711No Hit
AGCCTGGGGACACTCTCCAATTGTAAGAGAAAGGGGGCAGGGAAGCAATATA1250.12212278713509711No Hit
ACGCGGGGTGAACAATCTTATTTTTAACTAACACTATTTTCATACACGTATG1230.12016882254093555No Hit
ATTTTAGGGCGCTCCACTGTACTCCAGCCTGGGTGACAGCGTGAGACTCCAT1230.12016882254093555No Hit
CTGGGGGGTTCCCTTGAAGAAAACATTTAGCACAGCTTAAATAAAAGTTGAG1230.12016882254093555No Hit
TATTATGGGATTCCCCTTCCTGTGTCCATGTGATCTCATTGTTCAATTCCCA1220.11919184024385479No Hit
CTATGGGGTACAACTCAATGGCATTCCATCCCAAGGGATGGTTTAATGGCAT1220.11919184024385479No Hit
TGTGCAGGGATAATAAAAGATCAAAAGATATATCTCCTATGAACAGATTGGA1200.11723787564969324No Hit
GGCAATGGGAGTTTTCTTTTTTATTTTGGTTATGCTTAAATATGAATGTAAT1190.11626089335261246No Hit
TTGCCAGGGGTGTCAATGTAGATTTATCAATTTTAGTAAACATACAACTTTG1190.11626089335261246No Hit
TCGTACGGGAAGCTGTGACATTGTCTATAAGCATTTGGCTCCACAAATGTAT1190.11626089335261246No Hit
CACACTGGGACTCCTCGCTGCAGGGCACTGTCCCACCGGGATCCAGTTGCAA1190.11626089335261246No Hit
ACTGCAGGGAATAACATAGACTGACTCTATACTTACTTGCCTCAATAACATT1190.11626089335261246No Hit
TCATCGGGGTCGGGATGATGCGGACGCAGTGTCTGCTGGGGCTGCGCACGTT1190.11626089335261246No Hit
CAGACAGGGGCTGCCAGTCAAGGCCTGCATATGCAGAATGACGATGCCTGCC1180.11528391105553168No Hit
AGCTAAGGGATTGACAGGCATGTTTCCCATGCATAGAATTCTCAGCTGACAG1180.11528391105553168No Hit
AGATACGGGTCTTCCGATCGCCTCGTGGGGAGCCTATTAAACCTCTTTTTCT1180.11528391105553168No Hit
TAATAGGGACCTGCTGTTTGTTTTGTTCTTAACTTCATTGTATTATGGGGCT1180.11528391105553168No Hit
GTGGTTGGGTTACTATAATTCATTAATATGTAGCATTGAAATATTTTTATTC1180.11528391105553168No Hit
GCGGCGGGGAGAATGGAGGAATACATTATTTTATCGTATATTTTTGTCCAAG1180.11528391105553168No Hit
TGCGGGGGGGCATGAATCTGCTAAATCTCATTATTTCTCCTTAATATGTTAC1170.1143069287584509No Hit
TTGTGTGGGACTTACTACCAAGGAACCTAAATTGGACTTTTTGAATAAAAAG1170.1143069287584509No Hit
TAGCTGGGAATAGCAATGCAGGAAAAAGGCAACCTTGTTTGTGGAAGGTTTT1160.11332994646137012No Hit
GGGTATGGGAGAATTGTAGATATATAATGCTCAGACTACTTTTAAAAATCCT1160.11332994646137012No Hit
CGTTGGGGGATAACACATTATTATAGCTTGATTTTAATTTTTTGCATTAATT1160.11332994646137012No Hit
TTAAATGGGAAGGTTGAAGTAAATTCTGATGCTGTTAATATTGATTTTAATT1150.11235296416428935No Hit
AGCACAGGGGTTAGGTTATGGGATGTAGACCGAGGGATATGCATCCATACCT1150.11235296416428935No Hit
TTCCAAGGGAACATTAGGAGTGTAGCTCTTCATAATGAAATATCTTCTTTCT1140.11137598186720857No Hit
TCATTGTGGGGTCTTTTGGAGAATCTAAGTTTGCATCATTTTAAAAATGGCT1140.11137598186720857No Hit
TAGGCTGGGAACATGAACTAACATTTCTGCACTGTAACGTGCCGGGCACACA1140.11137598186720857No Hit
TCGTATGGGAAGCTCTAAGAATCTGCCCCCTCCTGAAGGGCAGGTAGAGAAG1130.11039899957012778No Hit
TCTGCAGGGGAAACATTCCATGTTTTAATCTGAGCCTTGCAGACTTTCATTT1130.11039899957012778No Hit
CTAGAAGGGTAAATGATAAAAGTAAGTCTTGCTCATTGAAGAAAAATAGAGA1130.11039899957012778No Hit
ACCCATGGGATCCATTTGCCGTCAGTACTCTAATGGCAACTGCTATTCATGT1130.11039899957012778No Hit
TCCGCGGGGGAATGGATGAAGACATGAACTAAGCTACATCTCCATTTCTCCA1120.109422017273047No Hit
TTACAGGGTGTCAGTCTAAGGACTCAGGATACAAATACTCAAAACATAAATT1120.109422017273047No Hit
AAGTAAGGGTAACGAGGAAAGACTGGGGTAATTATAAACAAATATGTTAACC1110.10844503497596625No Hit
CAACACGGGATTCCAAAATTGACCACATACTTGGAAGTAAAGCTCTCCTCAG1110.10844503497596625No Hit
TTTGATGGGAACGTCTGTTCAGTGTAGATGAAATAGCCTTATACTGGAAGAA1110.10844503497596625No Hit
AGCCTCGGGGATCATCATGCTTCCGGACGAGACCACTGACTTGAGAACGGTG1100.10746805267888547No Hit
AAACATGGGGAATCTGCTCCCCACCTGGATGCTGCTAGTCCTTTCTCCAGGC1100.10746805267888547No Hit
TCTAGAGGGGATTTGAAGGAGAAGGAAATACCAGAATTAAGAAACAAACTGC1100.10746805267888547No Hit
TGCCCAGGGATGAAGTTCATTGTGTAGAAGGATTTATTATGACCCCCTTTGT1090.10649107038180469No Hit
TTTGCTGGGATCTGTTTTCTTGTTTATAAATGAAGGATGATAATAGTATAAC1090.10649107038180469No Hit
GCACCAGGGGGTAGAAGCAATCTTGACCTTTTTTATTTTTGCAGGCCCCTGC1090.10649107038180469No Hit
GTACAGGGGCTTTTAAAAAAAGTCAACTAGAATTGAGAAGAGGTGATTTTTT1090.10649107038180469No Hit
TTGCCCGGGAAGCATAAGGAAAAAAGCAAACATCAGAAAGATTTCCACTTAG1080.10551408808472391No Hit
TTCAGAGGGCGATCTTTATACGGGGACAGTTTTGTCTCTAGGTTTCTGACTT1080.10551408808472391No Hit
TGCAGGGGAGCATACCTGTTGTTAGATTCTAGCTTTGTCCATTGTTTTTAAG1080.10551408808472391No Hit
ATAACAGGGCTCTGCATTCTTTATATTTTGGAGATTGTTTTTCCAATCTCCA1080.10551408808472391No Hit
CATCCCGGGACTGAAATCCCACCTTATGTGATGAAGTGTCCGAGCAATGGTT1070.10453710578764314No Hit
TTTGTCGGGACATATATTTATGAGAATATTTAGTCAAAGGAAACAAGTATAA1070.10453710578764314No Hit
TAGATAGGGGGTCTTCTATTGGCACATCCTTTATTTACAGACATACCTCAGA1060.10356012349056236No Hit
TGCACCGGGGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATTGC1060.10356012349056236No Hit
GTAGGCGGGGAGAGAGCTGGGGAAAGTAGCAGCAACTAGATTAAGACTGGGA1050.10258314119348158No Hit
AATCGCGGGATTCACAGCTACGTGTGCCCCTCTCATGGTCATGGGTCTGCCC1050.10258314119348158No Hit
TGACCAGGGACAAGAAAACGCATTCTCAAATTCATATCCACTTCAAACTCTC1050.10258314119348158No Hit
TGAACTGGGATACAGGGATTGGCATTCTTCCCGGTCACTGGTAACAATAGCA1050.10258314119348158No Hit
AATATGTGGGAGATTATGTCATTTTGGAAACTATTGTGTCCCTTGTATTTTA1050.10258314119348158No Hit
CTGTGGGAGACTTCAACAGTGAGGAGGATGCCAAAGCTGCCAAGGAGGCCAT1040.10160615889640079No Hit
GATGGTGGGAAAATAAAATTTTAATAAGAACGTGTTATGTGACCGTTATTTC1040.10160615889640079No Hit
CGCAAAGGGATTTTGCTTGAGGAAGATGAAGGGACCAAGTGTGACTGGTTTG1040.10160615889640079No Hit
CTGTTTGGGAGAATTATAAATGTCATTTGACTATGTCAATCTGTATATATTG1040.10160615889640079No Hit
GAAGTAGGGACATTTGAGGACAGGAATATGTTGAGCCTGATTTTCCTGGTGT1040.10160615889640079No Hit
TAGATAGGGAGACGAGATCATACCGGTAAAGCAGGAATGACAAAGCTTGCTT1030.10062917659932001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)