FastQCFastQC Report
Sat 31 Dec 2022
SRR5849638.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849638.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146046
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC12420.8504169919066596No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10730.7347000260191994RNA PCR Primer, Index 27 (95% over 22bp)
TTAATAGGGGGGAGCAAAGGGCCATAAGGAGAATTTTTTAATCAAGCCATGT1710.11708639743642416No Hit
CAGCGGGGATTCTAAAGCTAGAGACAGAACTGTACAAAAGAATTGTCAGCAC1600.109554523917122No Hit
GCCCACCTTGGCCTCCCAAAGTGTTGTGATTACAGGTGTGAGCCACCGTACC1570.10750037659367596No Hit
GCGCTTGGGAACAGAGATTTGGATTAACAGAGATTTGGATTATTCCCATTCA1520.1040767977212659No Hit
CGCAACGGGCAATGCTGACCATTAATCTCCTAAGAAATGTTGTAGGTCAGTT1500.10270736617230188No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1480.10133793462333786No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)