FastQCFastQC Report
Sat 31 Dec 2022
SRR5849637.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849637.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144891
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC12120.8364908793506842No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10150.7005266027565549RNA PCR Primer, Index 27 (95% over 22bp)
GCCGCAGGGACAGGTATTAAATCATCAAGTTTTGTACCTTCAATATATACAA1720.11870992677253936No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1690.11663940479394856No Hit
GCGCTTGGGAACAGAGATTTGGATTAACAGAGATTTGGATTATTCCCATTCA1670.11525905680822135No Hit
GCCCACCTTGGCCTCCCAAAGTGTTGTGATTACAGGTGTGAGCCACCGTACC1560.10766714288672176No Hit
CAACATGGGATCATTTGTATTATATCACATAGCAGGTGTTCTATCATATCAT1540.10628679490099453No Hit
GACAAAGGGAATCACTCAGATACACTTGGGACCAAGAGACAGTGAAAAATAG1480.10214575094381294No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)