FastQCFastQC Report
Sat 31 Dec 2022
SRR5849632.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849632.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95636
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC10391.0864109749466728No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10291.0759546614245683RNA PCR Primer, Index 27 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1350.14116023254841273No Hit
AATTGAGGGACCATCTGAACTAACTACCCAAAAATTTTCACTCTGATTCCTA1180.12338449956083483No Hit
TACTCTCGGGGAACACACGACATGCGGCTTTTTCTGCATCAACTGCTATGAC1040.10874566062988832No Hit
TCTCATGGGATTACATTTAAGCTCTTGGGACATTACAATTGAATGTTGCTAT1010.10560876657325693No Hit
TCAGACGGGATTGTGTTGAAATAGCAGTTCAGCCTGTTGGCCTTTGACGTCT970.10142624116441508No Hit
CTGTATGGGGTTGTCTTTCTTGGCTCTGGTTTGCTTAATTCTTAATTCTCTC970.10142624116441508No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)