FastQCFastQC Report
Sat 31 Dec 2022
SRR5849631.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849631.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94964
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10191.0730382039509707RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC9340.9835306010698791No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1420.1495303483425298No Hit
TACTCTCGGGGAACACACGACATGCGGCTTTTTCTGCATCAACTGCTATGAC1260.13268185838844193No Hit
CTGTATGGGGTTGTCTTTCTTGGCTCTGGTTTGCTTAATTCTTAATTCTCTC1100.11583336843435407No Hit
CGCAATGGGAATGCTGCAATGAATCTATCTGGTTGTTTTGTAAGCACAAAAT1070.11267427656796261No Hit
CGTCCAGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCAA1020.10740912345731013No Hit
TTAAGCAGGGGATAGAAAATAATACAAACTTAAAATTTAAAAACCAATATAC1020.10740912345731013No Hit
CTTGTTGGGACTCATTAGTATCGGCAAATTAAAGTGTTCCTGGATCCGTTCC1010.10635609283517966No Hit
TATGCAGGGAAATGACATAATATAATTCCAAAGTATGTAGTTTACGTTTAAA980.10319700096878817No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)