FastQCFastQC Report
Sat 31 Dec 2022
SRR5849630.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849630.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95854
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT9931.0359505080643479RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC9100.9493604857387277No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1410.1470987126254512No Hit
TACTCTCGGGGAACACACGACATGCGGCTTTTTCTGCATCAACTGCTATGAC1290.13457967325307238No Hit
TGCTCGGGGGGATGTAGCTATTGGTCTTATGTGGGGCCAAAAACCTGATTTG1220.1272769002858514No Hit
ACCTGCGGGAACTGAATAATATTATGTGGAATGGTTGACTGCTAATGTCAAA1070.11162810107037786No Hit
TTAAGCAGGGGATAGAAAATAATACAAACTTAAAATTTAAAAACCAATATAC1040.10849834122728316No Hit
TCTCATGGGATTACATTTAAGCTCTTGGGACATTACAATTGAATGTTGCTAT1020.10641183466522001No Hit
CGCAATGGGAATGCTGCAATGAATCTATCTGGTTGTTTTGTAAGCACAAAAT990.10328207482212533No Hit
GTAAATGGGACAGATATTCTTCTCTTTAATTTCTCAGATAAGTCATAGATGT970.10119556826006218No Hit
GCTAACGGGAGAGTAGGACCACAGTTGCTTGTATTTGTTTAGTTACTACTTT970.10119556826006218No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)