FastQCFastQC Report
Sat 31 Dec 2022
SRR5849626.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849626.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277674
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT23400.8427148382635754RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC17900.6446408378170084No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC3140.11308224752767633No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)