FastQCFastQC Report
Sat 31 Dec 2022
SRR5849620.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849620.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183125
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10980.5995904436860068RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC9930.5422525597269624No Hit
CAGCTAGGGATTGTCAAATAGGTAAAAGAATGTTATTGATAGAAGAAAAAAT2250.12286689419795221No Hit
CGAGATGGGGCTAACCTTTATTAAACAGATCGCATATTATGATCTTGCTGCA2060.1124914675767918No Hit
CTCTAGGGGGCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATCC1960.1070307167235495No Hit
TCTGCAGGGGTATTTGTATCCTTTCTTGTTCTTGTGATCTTGTGATGAAGAC1920.10484641638225256No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1850.10102389078498293No Hit
AAAAACGGGTAAATACTGTATCATAAGTAGAAGAAAATAATTTTTGTTTTCT1850.10102389078498293No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)