FastQCFastQC Report
Sat 31 Dec 2022
SRR5849619.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849619.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182861
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10780.5895188148374996RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC9710.5310044241254286No Hit
TTGCGTGGGGAAAATGTGCAGTAAAAATCAATAATAGCAAACATTTGTTGAA2130.11648191795954305No Hit
CTCTAGGGGGCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATCC2090.11429446410114787No Hit
CAGCTAGGGATTGTCAAATAGGTAAAAGAATGTTATTGATAGAAGAAAAAAT1930.10554464866756716No Hit
CGAGATGGGGCTAACCTTTATTAAACAGATCGCATATTATGATCTTGCTGCA1890.10335719480917199No Hit
AATTGTGGGAAGAGGAATTTAATACAGGAGGTACCTCCACAGTCTCAAAGCT1870.1022634678799744No Hit
CGTTTCGGGAACCTTCATCAAGAAATAGGTTATAGGTAAAATCTCTGAAGGA1840.10062287748617803No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)