FastQCFastQC Report
Sat 31 Dec 2022
SRR5849618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182918
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10980.6002689729824292RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC9410.514438163548694No Hit
AACTCAGGGTAATGTATACTTGGAAAAGTTACAAAATTACCCTTAGTTGATT2040.11152538295848412No Hit
CTTGCCGGGTCTTCCGATCTTTTGCAGGGATACTGAATTTATAGAAAGCAAG2020.110431996851048No Hit
CGAGATGGGGCTAACCTTTATTAAACAGATCGCATATTATGATCTTGCTGCA2010.10988530379732996No Hit
CAGCTAGGGATTGTCAAATAGGTAAAAGAATGTTATTGATAGAAGAAAAAAT1960.10715183852873966No Hit
AAAAACGGGTAAATACTGTATCATAAGTAGAAGAAAATAATTTTTGTTTTCT1940.10605845242130355No Hit
TTGCGTGGGGAAAATGTGCAGTAAAAATCAATAATAGCAAACATTTGTTGAA1910.10441837326014936No Hit
CTCTAGGGGGCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATCC1840.10059152188412294No Hit
TACGCAGGGATTTATAAAATTCTAAAATGAAAGATAAAATAATTGCTATTAA1830.10004482883040487No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)