FastQCFastQC Report
Sat 31 Dec 2022
SRR5849617.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849617.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177635
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC10090.5680186900104146No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT8430.47456863793734344RNA PCR Primer, Index 27 (95% over 22bp)
GCGGGCTCAAGGAGGGCAAACTCAATCTCCCACCAAAAGTCACACTCCAAGT2080.11709404115180003No Hit
GCGTTGGGGATGAAGACCAATGTATGGAGATAAATTATGTAATTTGATAATT1980.11146451994257889No Hit
CAATCCGGGATTTCTCAATGGGCAAAAACTGCCCACTGTCACCTTTGGATTT1970.11090156782165676No Hit
ATCAGAGGGATATTTTAAATGTAGTTGTAAAATGACAGAACATGGGATCACA1960.11033861570073467No Hit
AGCAATGGGATTTCATGATTGATATGTCACAGAAATCATGGTAGTAAATCAC1950.10977566357981254No Hit
ACAGACGGGATTAAAGGATTTTAAGCAAAGATGAAACATCATTTTTCCATTT1910.10752385509612407No Hit
GAACGGGGGATCAAAGATTTGTTTGGTATAAATAAAGAATTATTTGTTTTGT1850.10414614237059137No Hit
TTGAAAGGGAGTGCAGCCCGGAGGCTTAGGGTGCAGCCTCCCTGGTCTTCCT1840.10358319024966926No Hit
TGGTGACGGGAATGCATCTCTGCTACAGCAGTCATTGTCATCCTGTGACCTT1820.10245728600782504No Hit
TTTAAGGGCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAG1800.1013313817659808No Hit
TTATCTGGGGGATGAAAGATAAAGCCCTTAAGGACAAGATTGAAAAGGCTGT1800.1013313817659808No Hit
AATGCTGGGATAAAAATATAGGCCATCTGGAGAATGCAAGAGGGAAAGCATG1790.1007684296450587No Hit
TCAACCGGGACCTATAGAATGGCTACAAAGATTTAAGGGGCTAAAATGTTAG1790.1007684296450587No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)