FastQCFastQC Report
Sat 31 Dec 2022
SRR5849616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176107
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC9810.5570477039527105No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT8180.4644903382602622RNA PCR Primer, Index 27 (95% over 22bp)
AATGCTGGGATAAAAATATAGGCCATCTGGAGAATGCAAGAGGGAAAGCATG2090.11867784926209635No Hit
GCGTTGGGGATGAAGACCAATGTATGGAGATAAATTATGTAATTTGATAATT2070.11754217606341599No Hit
AAACAGGGAAGTTTGAAAATCTGAAACCTTAAGTGTTAACATGAGCACCTTA2020.11470299306671511No Hit
CAATCCGGGATTTCTCAATGGGCAAAAACTGCCCACTGTCACCTTTGGATTT1980.11243164666935443No Hit
ATCAGAGGGATATTTTAAATGTAGTTGTAAAATGACAGAACATGGGATCACA1970.11186381007001425No Hit
ACTTTCGGGATATTAGAGCTAGGAAACAGGGGCAGCCTGGATTTTTATGGTA1960.11129597347067408No Hit
TTGAAAGGGAGTGCAGCCCGGAGGCTTAGGGTGCAGCCTCCCTGGTCTTCCT1940.11016030027199374No Hit
TGGTGACGGGAATGCATCTCTGCTACAGCAGTCATTGTCATCCTGTGACCTT1900.10788895387463303No Hit
CGCGTTGGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1880.1067532806759527No Hit
ACAGACGGGATTAAAGGATTTTAAGCAAAGATGAAACATCATTTTTCCATTT1870.10618544407661251No Hit
ACAAATGGGATCTTAAAAGGGCAAAACATAAGAAGAGGTAGGAGTATTCTAA1870.10618544407661251No Hit
TCAACCGGGACCTATAGAATGGCTACAAAGATTTAAGGGGCTAAAATGTTAG1850.10504977087793217No Hit
TGACCTGGGACTCATATTTTATTATTTTTGATTTATAACCAAAAGGACTATA1840.10448193427859198No Hit
TTTAAGGGCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAG1810.10277842448057146No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)