FastQCFastQC Report
Sat 31 Dec 2022
SRR5849615.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849615.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179397
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC9770.5446021951314682No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT7460.41583750006967785RNA PCR Primer, Index 27 (95% over 22bp)
ATCAGAGGGATATTTTAAATGTAGTTGTAAAATGACAGAACATGGGATCACA2210.12319046583833619No Hit
TCAACCGGGACCTATAGAATGGCTACAAAGATTTAAGGGGCTAAAATGTTAG1970.10981231570204629No Hit
GCGTTGGGGATGAAGACCAATGTATGGAGATAAATTATGTAATTTGATAATT1960.1092548927797009No Hit
TGGTGACGGGAATGCATCTCTGCTACAGCAGTCATTGTCATCCTGTGACCTT1940.10814004693501006No Hit
ACAAATGGGATCTTAAAAGGGCAAAACATAAGAAGAGGTAGGAGTATTCTAA1910.10646777816797383No Hit
TTATCTGGGGGATGAAAGATAAAGCCCTTAAGGACAAGATTGAAAAGGCTGT1880.1047955094009376No Hit
AATGCTGGGATAAAAATATAGGCCATCTGGAGAATGCAAGAGGGAAAGCATG1870.10423808647859217No Hit
CTGCGAAAGGGAATCTAGCAGGATGACGCAGTGGAAGATTAAGGTAAAAATT1820.10145097186686511No Hit
CAATCAGGGACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCT1810.10089354894451968No Hit
CAATCCGGGATTTCTCAATGGGCAAAAACTGCCCACTGTCACCTTTGGATTT1800.10033612602217427No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)