FastQCFastQC Report
Sat 31 Dec 2022
SRR5849611.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849611.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137215
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT14321.043617680282768RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC14031.0224829646904494No Hit
TCGACTGGGGTCACCATTTTGCATTATTTAGAATAGATTTTTAAATGAGCTA2010.14648544255365667No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1830.13336734322049337No Hit
TAGTCAGGGGGAGAGATTAGTGAAGGCTGGAGTAATCACAGATGGCTTCATG1740.12680829355391177No Hit
CGCGAAGGGAGTTTAAAAAATGAGAGTTTTGAAAAGCCAGGGATGCTCTCGG1720.12535072696133806No Hit
GCCTGGGGCACCACTCTACGTGGAATGAATCAGCAATGGACATTCCTGCAAA1700.12389316036876434No Hit
CTGTTCTGGGGGTACCTGAGCCCTGAGGGCCTCACGGCAGTGTCAGGAAGCC1660.12097802718361696No Hit
CTGTGCGGGACTGCTACAGTGCACTGTATGGTGTGCTGATGAGAACAGACAA1560.11369019422074846No Hit
GCTCAGGGATCCTTGATGGTATTAAATGTTTTTGAATGAGAACTATAGAAGT1530.11150384433188791No Hit
TTCGAAGGGCTTGGTGAGACTGGAAAAAGAAGAAGAAAAGAATGGGGCTAGT1460.10640236125787997No Hit
TGCTGTGGGAACATTCAAAGATACATAAGTTCCCTTTAGAAACCTTTATTTA1420.10348722807273257No Hit
AGACATGGGGTGGGTTTACATTCTGGTGATAGGAGATAGGCAGTAAATTATT1390.10130087818387203No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)