FastQCFastQC Report
Sat 31 Dec 2022
SRR5849610.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849610.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136225
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC15221.1172692237107726No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT14821.087906037805102RNA PCR Primer, Index 27 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC2080.15268856670948797No Hit
TCGACTGGGGTCACCATTTTGCATTATTTAGAATAGATTTTTAAATGAGCTA1950.14314553129014498No Hit
GCCTGGGGCACCACTCTACGTGGAATGAATCAGCAATGGACATTCCTGCAAA1720.12626169939438428No Hit
GCTCAGGGATCCTTGATGGTATTAAATGTTTTTGAATGAGAACTATAGAAGT1690.12405946045145899No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT1680.1233253808038172No Hit
AGACATGGGGTGGGTTTACATTCTGGTGATAGGAGATAGGCAGTAAATTATT1620.1189209029179666No Hit
TAGTCAGGGGGAGAGATTAGTGAAGGCTGGAGTAATCACAGATGGCTTCATG1490.1093778674986236No Hit
GCTTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1460.10717562855569829No Hit
CTGTTCTGGGGGTACCTGAGCCCTGAGGGCCTCACGGCAGTGTCAGGAAGCC1440.10570746926041474No Hit
CGCGAAGGGAGTTTAAAAAATGAGAGTTTTGAAAAGCCAGGGATGCTCTCGG1440.10570746926041474No Hit
CTGTGCGGGACTGCTACAGTGCACTGTATGGTGTGCTGATGAGAACAGACAA1400.10277115066984767No Hit
GTGAACGGGAGACAGGTGGAAGAGGACACAGTGAAAAGGCTGTTGGATAACT1390.10203707102220591No Hit
ATGTGGGAACATATTATTAACTATAGTCATGCTACAGTGGTATAGAACACTA1390.10203707102220591No Hit
ACGAGTGGGCTTGAATGTGATTGACCTACTTGAAAAATATTAATACCTTTAA1380.10130299137456414No Hit
TTCGAAGGGCTTGGTGAGACTGGAAAAAGAAGAAGAAAAGAATGGGGCTAGT1370.10056891172692238No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)