FastQCFastQC Report
Sat 31 Dec 2022
SRR5849596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172821
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC11110.6428616892623004No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT9480.548544447723367RNA PCR Primer, Index 27 (95% over 22bp)
TGAGACGGGAATGTTTAACTTGTAACTAATGCAGAATTGATTTTTTTAATCC2830.16375324757986587No Hit
ATAGTTGGGATTTGCCACATAGTGTTATCCTCAGGAATGAATGAGACTATAT2660.1539164800574004No Hit
CTTGTTGGGATGCACTAGTTGTTCACCTGAAAGATTAAGTGTACCTGCAGAG2600.1504446797553538No Hit
AACAATGGGATCACACTGTTAGGTATTTTCAGTTTTATTTTTGAGGACTTTT2590.14986604637167936No Hit
CCGCTTGGGATGTCATGGCAGAAAACTTCAGCAATAATTATTTCAATGGTAA2550.14755151283698162No Hit
CCAGGTGGGGAAACCTCTCTGGTGGGAAACGTGAAATTAAACAAAACAAAAC2500.14465834591860943No Hit
TGGCTGGGGGATTCCTGGCCCATGGGAAGATTGCGTTTCACCTGCTCCTGAA2490.144079712534935No Hit
ATAGCCGGGAGAGTGAGCTCAGTGAATGCTGGGGTATCATGATGGAGTCACA2480.14350107915126054No Hit
CTTTAGGGGACTTAACACCTGTTTTCGATGAGGTTCCCGGTACTGTCACCTT2460.1423438123839117No Hit
TTTGAAGGGTTATTCTTGAAATTAAAGTTAAACCTTGCTCTTCTAACATTAG2420.14002927884921393No Hit
TTCTTGGGATGGAGGAGCCTTCAATATCTTCCTATCCCTAAAGAGGAGGGAG2410.13945064546553948No Hit
TACTTGGGGATTGTGCTGGCTATGACTTCAAAATGAGTCTGGAAGGGGCACA2390.1382933786981906No Hit
AAAAGTGGGGGAAGCCAGGTATCATGATTCCTGGCTGAGGACTAGGCTACGT2380.1377147453145162No Hit
AGTCCAGGGCAGAGGAAGAACGCAGGGAAAAGAAAAATAAAATAATGCAAGC2340.13540021177981842No Hit
CCGAGGGCTAGGGAGGGATCCGTGGACTGCTCTGGAAAGTGAGGAGTTGGGG2300.1330856782451207No Hit
GACACTGGGAGAAAGATTTTACTTTTGATTTTATTTGATCAATTTGTTCTTA2270.13134977809409737No Hit
CACGTAGGGGTGTAAACTGGAAAAATTCACATCATTTCTGAGTTTAATCACT2270.13134977809409737No Hit
AAGATGGGGATAATTAGAGCAAGAAAGTCCTGCTTGACATTGTATTGGAGCC2250.13019251132674847No Hit
CTTGCAGGGACCCCTCTATTGCCCTTCAATTCATGAAGTTTAAATGATCACC2200.1272993444083763No Hit
GGTCGCGGGGACCAGAATCTAAGAAAGATGAAAATTAATTTCAAATACTGAA2200.1272993444083763No Hit
TTCAATGGGGATGGGAGCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTT2190.12672071102470187No Hit
AAAAGCAGGATGGGCAATATGTAAATAAATAAATGTAATATTTCATCCTTTA2190.12672071102470187No Hit
ATAAATGGGCTCCTTTGGAAAGGGAGAGAAGAGTAATCATTGTGAAATACAC2180.12614207764102742No Hit
ATTTTAGGGGTATCCAGCAGTGATCAATTGCTCTTTATAGGAAACCACCTTT2160.12498481087367855No Hit
AAGCATGGGAGATGTGGACATGCACCCGCATGTACAAAAACACAAATACGGA2150.12440617749000411No Hit
TAAGTCGGGGGGACTCCCCTGTCTTGCTCCTCGAGACCCTCCCCCATCACCG2150.12440617749000411No Hit
GTACAGGGGGGAGGAGGTGGCACCAGAAATGTGTATTTGAGGATTTGATCAG2140.12382754410632967No Hit
ACCCTGGGGATCTAACTCAGGGATTCCCTTTTATAGGCGAAGAGAAAGGCAT2140.12382754410632967No Hit
AGCAAAGGGAGGAGGAACTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGG2130.12324891072265523No Hit
TTAATAGGGAAGTCCAAAACTGTCACACAAAAAAAGAAGGACATAATCTAGT2120.1226702773389808No Hit
TAATGTGGGATTATCTTTTGTCTTTGTCAGTATAGCCATCAAAAAGTTTTTT2120.1226702773389808No Hit
TTTTGTGGGATGTGAGATAGTTTTCATTTTAACTGGCATGTCATTTGCACAC2110.12209164395530635No Hit
GAAACAGGGGACGAAGTTCCCGTAAGCAATTGCAACCAAAACAAAAATGGAC2110.12209164395530635No Hit
GAAAGCGGGAAGTAGAGAGCTGATAGTATGTTTTTTCTAAAGTATACAGTTT2100.12151301057163191No Hit
GAAATTGGGAACTTCACCATTTCACCTATAAACCTTGATTTTTTAAAATTTT2100.12151301057163191No Hit
ACTGCAGGGAGTGGCCTCGAAGTTCTTGCCTGGTTGAGAAATTATAATGAAT2090.12093437718795749No Hit
CCAACAGGGCATTATTGTTCTCAACTTGGGGCTCTCCCCAACCTGGGGACCC2090.12093437718795749No Hit
TTAACCGGGATGTTACATATCAGCAGAAGGCAAAAACTATATAAATGAACAT2080.12035574380428304No Hit
AATGGTGGGAATTCCGTCAGATGTGGGCCGAATTTGAATGGGAAAACAAAGT2080.12035574380428304No Hit
TTACGTCGGGGAAGAAACAAAAGATAGACTCACTGAAAAGTTACAAGAAACC2070.11977711042060861No Hit
CTAGTAGGGATTGCCCTCTGCCCAGCTGACAGTGGCATCCCCAGGGAAGGGG2060.11919847703693418No Hit
GAAAAGGGGATTCTCCTTTCTACATATATACCATCATGTGCCACATAACAGC2060.11919847703693418No Hit
CATATAGGGAAACATTGACCATTTAGAAAATAAATTGATGTAACACTATCTG2050.11861984365325973No Hit
GAATAAGGGGAGGTATTGATAGCTTGCTTTATTTCTGCCTTTACGTTGACAG2040.11804121026958529No Hit
TGGCTCGGGATAAACTTCATGGAACTACCAGACTTTTAATAGCATATATTAA2040.11804121026958529No Hit
GCTTAGGGACCCGTTCTTTGCTTGGACATCCCATTTTCTTTTGTCCAGACCC2030.11746257688591086No Hit
ACCGCAGGGTCAGTAGCGGGAGTGTGGTAAGTGTGGAAAAGCCTTTAGATCA2020.11688394350223641No Hit
CATAGAGGGATGGATATATAAGTAAGAGAATGTTCAGATTTCATAGAGGAGG2010.11630531011856199No Hit
TATATCGGGAGGTTGTAGTTGCATCTTTACAGTGGTCTTTCCTTTTATTAAA1990.1151480433512131No Hit
CACAGGGAGCAGGGGGCTAATTACCATTACACTTGACCTCTTCCATCCCCAT1990.1151480433512131No Hit
GTTTTGGGGAGGTAGCTATGATCATCCTCATTTTTTCTATATGAGATTTGGG1980.11456940996753867No Hit
ATAGATGGGACACGACGCTCTTTCGATCTTGTGTTGGGATTCCAGCTTTTCC1970.11399077658386424No Hit
GTAGCAGGGACTGGCCCCACCCTACCCTGGCCTTGCCCTGCCCTGGCCCTGC1970.11399077658386424No Hit
CATGAGGGGAGCTTGAGCCCCTTAGCACCATGTAAGGAGCACAGCATCCAAA1970.11399077658386424No Hit
ATTCAGGGGAAGCTTTGATGAGATTCTATTTTCAGTAAGTTAGTGCTTCTGG1950.11283350981651535No Hit
GAAAACGGGGGGCTACAAGACAAGCTGTGCATAAGGTTGATATTTAGACATC1950.11283350981651535No Hit
CTCAAAGGGAAACTTTCTAATTTCACTGGTTTTTTTGTTTGCTTGCTCTTTA1940.1122548764328409No Hit
GAATAAGGAGTTTTGGGGGAGTTAGTATTGATGAATGCTCTTATCATTCTCA1940.1122548764328409No Hit
CGTAATGGGAGTTTCATCTTAGATGGCAAAGAAAAGCCAGGCTTCTCTATAC1940.1122548764328409No Hit
TATTGTGGGAGAAAGTTTGCTGAAAGCAAAGGGCTAAGCTTATGAAGGAATG1930.11167624304916647No Hit
CAAATAGGGATTAATCATTATTTCCTGGGGAGAAATATGGAATGAATTTGAT1910.11051897628181759No Hit
ATGAGGGGATATTTTCAGGTCTTGCCTTTAAATCTTTATTGTTTTTAGAGTT1900.10994034289814317No Hit
CAACTGGGTCTTATATTCAACTTTGAATCAATTACTGTGCACAGGGGATGAC1890.10936170951446873No Hit
GTGACCGGACTCCAGAATACACCCCATATTTGTCTCGTTCTCTCCACATTCA1890.10936170951446873No Hit
CTTAGCCGGGAAATTGGTTTTGGTATAAATGTAGCAGAAGAAAAACTAAATT1890.10936170951446873No Hit
CTCCAGGGTCCAGTGGGGATTCCTTATTGCTCAGCACTTTCCTTTCCTGCTG1890.10936170951446873No Hit
ATTTTTGGGTGTCACCTGCCTGAATATTAATCCCCCTGACTTATTGCTCCAC1890.10936170951446873No Hit
CTTGGCGGGGGAAGCTAAGAAAGCTGCAAAGCAGGAAGCAAGAAGTGACAAG1890.10936170951446873No Hit
CATGGGGGTCTAGAGTGGGGTTTGAAGACAGTTCACGTGTGCCTCTGTAAGC1880.10878307613079428No Hit
TTATTAGGGAAAACCAAATGGAGAAGAACAAAAAGATGAAGGCAACTCGAAA1870.10820444274711985No Hit
ACAAGTGGGCATATTGGAAGGTCACTGTTTGTTAGGATAAATAAATACTGGA1860.10762580936344542No Hit
AATAAAGGGGCACTTATGGAGATTTTTGACTTCACAGAAATGGAAGGTAACC1860.10762580936344542No Hit
GTAGCCGGGATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG1860.10762580936344542No Hit
TTTAGCGGGAAGGATAAAATTGGAGAAAAGGAAATTAAATATAGCCAGTAGT1860.10762580936344542No Hit
GCTACCGGGGGAACAAGGGAGGGAATAAATAGTTGTCTCCACTAAATCAGGA1850.10704717597977097No Hit
GCTTTAGGGCTTTTAATAGTTTCTGAAAATTTAAAGAAAAATCAATTGCTAT1850.10704717597977097No Hit
CTAGTAGGGGGGAGGGAAAGAGCACATCTTCATCCCAGGCCTCAGTAAATGG1850.10704717597977097No Hit
GCTTTGGGGACCTATCTAAACTTTTATTTAAATTTCTGTTTAAACTTCTATT1840.10646854259609653No Hit
AATCACGGGTCCACGTGGAAAGTTGGCTTAAGCCAAAATATATTCAAAGGCT1840.10646854259609653No Hit
TAAATAGGGGTGCCTGGCCTGCTTTCTTTTACTAATAGGCTTAATCAACTTT1830.10588990921242211No Hit
AAGAAGGGGAGTGAAAAATGTAGCTGCTGACTGTTTCTGAACTGGATATCGC1830.10588990921242211No Hit
GCCAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1820.10531127582874766No Hit
AATTAAGGGATGGTAACTGTACATTTTGTGCTAAAATGCATATTTATATGTG1820.10531127582874766No Hit
GAACATGGGGGCACAGAAGAAAATGAATGTAAATTCCTTATGTTGTGCAAGA1820.10531127582874766No Hit
GTAGTTGGGCATTCCTGATGATATGGTACATCCATTTCTGTCTCAGTCATTG1820.10531127582874766No Hit
AACGTGGGGACAATTACTTGTTACTGAATAACTACTTATTCAAAATATCATG1820.10531127582874766No Hit
ATAGCGGGGTGCTGGCCAATGTGGCCAGCTCCATCCTGAGCCACATTAAGGC1820.10531127582874766No Hit
GGACTAGGGGCTGCTTGTCAGGAACCATAATTCATTATTACATATAAGGGGT1810.10473264244507323No Hit
TAGCTAGGGAACCTAAAAATAGCTGCTAAATTCTAACTTCAGGTCAGCAATG1810.10473264244507323No Hit
TTACTGGGGAGTGAAACGAAGATGGGCTTTTCCAGATCACCTGCAATAGCAG1800.1041540090613988No Hit
ACTTGCGGGGTGAGGACCAGCAAGTGTTTTCACAAATGTGTTTGACTCAAGT1800.1041540090613988No Hit
CATGTTGGGCCAAACATCAGCAACAAGTCATGAGGTATAATGAACATATCAA1800.1041540090613988No Hit
GCACAGGGGATCTTATACGGGGGGAAATAATCTAAGTGAGAGGTTGGAGACT1800.1041540090613988No Hit
CAGCCTGGGAAGTTTGTTTATTCCTGTAGCCTAAAAATCTATGCTTCAGGAT1800.1041540090613988No Hit
ACAAGGGGGGTACCCTCCAGATTCCTATAAGACGCCCTGTCCAGGTGTCCCC1800.1041540090613988No Hit
AGTTTTGGGTATACGAGGGCGCATCCATCAGAGCCTCCTCTTGACGTTTGTC1790.10357537567772435No Hit
TATTTAGGGAGAGTAACTGGTGGGTTACTAGATCGACTGGATGACAGCCCTG1780.10299674229404991No Hit
AATTAGGGATCTTTGAGTCTGTGTAACCAGGAGTAATTGGGAGATGTTTCTG1780.10299674229404991No Hit
ATCAGGGGGCGATCTGAATCTGGGACAGTCTCCGGGATCCCCAGGCCTGGAG1780.10299674229404991No Hit
ATGCAAGGGAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATGGCGCCCTG1770.10241810891037549No Hit
TAATAGGGGCACGTATGTGGTAAAAACAGTAAAAGCAAGGTATCAAGTAAAA1770.10241810891037549No Hit
CTGATGTTGGGGGGATGCTTCATAAGTCATGATGTTTTTTAATATATAAATG1760.10183947552670103No Hit
TAAAACGGGATTTAACAAATACTTATATAGTTTTATGTGCCACACACTATTA1750.10126084214302661No Hit
TCTGCAGGGATTCATGCCCTTGACCATGTGGTTGCTTGGAGACCTGGGGTCT1750.10126084214302661No Hit
GGCTCCGGGATACTTAACCTGCAGAAAATTACAGACAAAGAAAAAACACCAG1750.10126084214302661No Hit
TTGAGTGGGGTCTGCAATGGGGGGCCTGCATGGCACTGCCCAGTAGAAATAG1730.10010357537567773No Hit
ACCAAGGGGCGCTGCGGGCTGTGCAACAATTTGAATTTATATCAGTTTACAA1730.10010357537567773No Hit
TCACAGGGATGGAAGAAACTGCTCTCAGGGACATGGATGGAAAATATTAGTG1730.10010357537567773No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)