FastQCFastQC Report
Sat 31 Dec 2022
SRR5849595.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849595.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171004
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC11810.6906271198334542No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT9490.5549577787654091RNA PCR Primer, Index 27 (95% over 22bp)
TGAGACGGGAATGTTTAACTTGTAACTAATGCAGAATTGATTTTTTTAATCC2870.16783233140745246No Hit
ATAGTTGGGATTTGCCACATAGTGTTATCCTCAGGAATGAATGAGACTATAT2830.16549320483731375No Hit
TTTGAAGGGTTATTCTTGAAATTAAAGTTAAACCTTGCTCTTCTAACATTAG2720.1590606067694323No Hit
ATAGCCGGGAGAGTGAGCTCAGTGAATGCTGGGGTATCATGATGGAGTCACA2650.15496713527168954No Hit
ATAAATGGGCTCCTTTGGAAAGGGAGAGAAGAGTAATCATTGTGAAATACAC2560.14970410048887745No Hit
AACAATGGGATCACACTGTTAGGTATTTTCAGTTTTATTTTTGAGGACTTTT2540.1485345372038081No Hit
CCAACAGGGCATTATTGTTCTCAACTTGGGGCTCTCCCCAACCTGGGGACCC2540.1485345372038081No Hit
TGGCTGGGGGATTCCTGGCCCATGGGAAGATTGCGTTTCACCTGCTCCTGAA2530.1479497555612734No Hit
GAAACAGGGGACGAAGTTCCCGTAAGCAATTGCAACCAAAACAAAAATGGAC2450.143271502420996No Hit
CTTTAGGGGACTTAACACCTGTTTTCGATGAGGTTCCCGGTACTGTCACCTT2430.14210193913592664No Hit
AAAAGCAGGATGGGCAATATGTAAATAAATAAATGTAATATTTCATCCTTTA2400.1403475942083226No Hit
CATAGAGGGATGGATATATAAGTAAGAGAATGTTCAGATTTCATAGAGGAGG2390.13976281256578793No Hit
CCGCTTGGGATGTCATGGCAGAAAACTTCAGCAATAATTATTTCAATGGTAA2380.13917803092325326No Hit
TTCTTGGGATGGAGGAGCCTTCAATATCTTCCTATCCCTAAAGAGGAGGGAG2380.13917803092325326No Hit
TAATGTGGGATTATCTTTTGTCTTTGTCAGTATAGCCATCAAAAAGTTTTTT2380.13917803092325326No Hit
CTTGTTGGGATGCACTAGTTGTTCACCTGAAAGATTAAGTGTACCTGCAGAG2370.1385932492807186No Hit
TTAACCGGGATGTTACATATCAGCAGAAGGCAAAAACTATATAAATGAACAT2320.13566934106804518No Hit
AAGCATGGGAGATGTGGACATGCACCCGCATGTACAAAAACACAAATACGGA2280.13333021449790647No Hit
CCGAGGGCTAGGGAGGGATCCGTGGACTGCTCTGGAAAGTGAGGAGTTGGGG2280.13333021449790647No Hit
TATATCGGGAGGTTGTAGTTGCATCTTTACAGTGGTCTTTCCTTTTATTAAA2270.13274543285537183No Hit
TGGCTCGGGATAAACTTCATGGAACTACCAGACTTTTAATAGCATATATTAA2260.13216065121283713No Hit
ACCCTGGGGATCTAACTCAGGGATTCCCTTTTATAGGCGAAGAGAAAGGCAT2250.13157586957030246No Hit
GACACTGGGAGAAAGATTTTACTTTTGATTTTATTTGATCAATTTGTTCTTA2240.13099108792776776No Hit
GAAATTGGGAACTTCACCATTTCACCTATAAACCTTGATTTTTTAAAATTTT2240.13099108792776776No Hit
ACTGCAGGGAGTGGCCTCGAAGTTCTTGCCTGGTTGAGAAATTATAATGAAT2220.12982152464269842No Hit
TTCAATGGGGATGGGAGCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTT2200.12865196135762905No Hit
CTCAAAGGGAAACTTTCTAATTTCACTGGTTTTTTTGTTTGCTTGCTCTTTA2200.12865196135762905No Hit
ATCAGGGGGCGATCTGAATCTGGGACAGTCTCCGGGATCCCCAGGCCTGGAG2200.12865196135762905No Hit
GTTTTGGGGAGGTAGCTATGATCATCCTCATTTTTTCTATATGAGATTTGGG2170.12689761643002503No Hit
ATAGCGGGGTGCTGGCCAATGTGGCCAGCTCCATCCTGAGCCACATTAAGGC2170.12689761643002503No Hit
CCAGGTGGGGAAACCTCTCTGGTGGGAAACGTGAAATTAAACAAAACAAAAC2160.12631283478749034No Hit
TAAGTCGGGGGGACTCCCCTGTCTTGCTCCTCGAGACCCTCCCCCATCACCG2140.125143271502421No Hit
ACCGCAGGGTCAGTAGCGGGAGTGTGGTAAGTGTGGAAAAGCCTTTAGATCA2130.12455848985988632No Hit
ACCAAGGGGCGCTGCGGGCTGTGCAACAATTTGAATTTATATCAGTTTACAA2100.12280414493228228No Hit
TACTTGGGGATTGTGCTGGCTATGACTTCAAAATGAGTCTGGAAGGGGCACA2090.12221936328974761No Hit
GTAGCAGGGACTGGCCCCACCCTACCCTGGCCTTGCCCTGCCCTGGCCCTGC2090.12221936328974761No Hit
GAAAACGGGGGGCTACAAGACAAGCTGTGCATAAGGTTGATATTTAGACATC2090.12221936328974761No Hit
AGTCCAGGGCAGAGGAAGAACGCAGGGAAAAGAAAAATAAAATAATGCAAGC2090.12221936328974761No Hit
AAGATGGGGATAATTAGAGCAAGAAAGTCCTGCTTGACATTGTATTGGAGCC2060.12046501836214359No Hit
ATTTTAGGGGTATCCAGCAGTGATCAATTGCTCTTTATAGGAAACCACCTTT2050.1198802367196089No Hit
CATATAGGGAAACATTGACCATTTAGAAAATAAATTGATGTAACACTATCTG2050.1198802367196089No Hit
AGCAAAGGGAGGAGGAACTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGG2040.11929545507707422No Hit
GAATAAGGAGTTTTGGGGGAGTTAGTATTGATGAATGCTCTTATCATTCTCA2040.11929545507707422No Hit
CTTGCAGGGACCCCTCTATTGCCCTTCAATTCATGAAGTTTAAATGATCACC2030.11871067343453956No Hit
GCTTAGGGACCCGTTCTTTGCTTGGACATCCCATTTTCTTTTGTCCAGACCC2020.11812589179200488No Hit
GTACAGGGGGGAGGAGGTGGCACCAGAAATGTGTATTTGAGGATTTGATCAG2000.1169563285069355No Hit
GGCTCCGGGATACTTAACCTGCAGAAAATTACAGACAAAGAAAAAACACCAG2000.1169563285069355No Hit
AATCACGGGTCCACGTGGAAAGTTGGCTTAAGCCAAAATATATTCAAAGGCT1980.11578676522186616No Hit
CATTTAGGGGACTGCAGTCCCAGCTGCTTGGAAGGCTGAAGCAGTAGGGGTT1970.11520198357933148No Hit
CACGTAGGGGTGTAAACTGGAAAAATTCACATCATTTCTGAGTTTAATCACT1970.11520198357933148No Hit
ATAGATGGGACACGACGCTCTTTCGATCTTGTGTTGGGATTCCAGCTTTTCC1950.11403242029426214No Hit
CAACTGGGTCTTATATTCAACTTTGAATCAATTACTGTGCACAGGGGATGAC1950.11403242029426214No Hit
GTAGTTGGGCATTCCTGATGATATGGTACATCCATTTCTGTCTCAGTCATTG1950.11403242029426214No Hit
AATAAAGGGGCACTTATGGAGATTTTTGACTTCACAGAAATGGAAGGTAACC1950.11403242029426214No Hit
CATGAGGGGAGCTTGAGCCCCTTAGCACCATGTAAGGAGCACAGCATCCAAA1940.11344763865172745No Hit
TAATAGGGGCACGTATGTGGTAAAAACAGTAAAAGCAAGGTATCAAGTAAAA1940.11344763865172745No Hit
GCACAGGGGATCTTATACGGGGGGAAATAATCTAAGTGAGAGGTTGGAGACT1920.11227807536665808No Hit
TTTTGTGGGATGTGAGATAGTTTTCATTTTAACTGGCATGTCATTTGCACAC1920.11227807536665808No Hit
CGTAGAGGGTAATCAGAGCCTCTTGAGAAATTGTTACTCATTGAACTGGAGC1910.11169329372412343No Hit
CTTGGCGGGGGAAGCTAAGAAAGCTGCAAAGCAGGAAGCAAGAAGTGACAAG1910.11169329372412343No Hit
GAAAAGGGGATTCTCCTTTCTACATATATACCATCATGTGCCACATAACAGC1910.11169329372412343No Hit
AAAAGTGGGGGAAGCCAGGTATCATGATTCCTGGCTGAGGACTAGGCTACGT1900.11110851208158874No Hit
ACTTGCGGGGTGAGGACCAGCAAGTGTTTTCACAAATGTGTTTGACTCAAGT1900.11110851208158874No Hit
CTTAGCCGGGAAATTGGTTTTGGTATAAATGTAGCAGAAGAAAAACTAAATT1880.10993894879651937No Hit
GTGAGGGGATCCCGCCATGCCCAAGCCCCACCCGACCTGTCTGGGTGGGCTC1880.10993894879651937No Hit
AGAAGTGGGCTCTGTTACACAGCCTTTGAAGTTATGTTTTTAGTAGTAAATC1870.10935416715398472No Hit
ACGGGGGATCTCTTGAGAATTATTTCAATTCCAGATACTTGTTAGAGATTTT1870.10935416715398472No Hit
ATTTATGGGTCACCTGTATATTTAAACTGTTCTTGGCATCTTGAAACACCTA1860.10876938551145003No Hit
CATGTTGGGCCAAACATCAGCAACAAGTCATGAGGTATAATGAACATATCAA1860.10876938551145003No Hit
TTGAGTGGGGTCTGCAATGGGGGGCCTGCATGGCACTGCCCAGTAGAAATAG1850.10818460386891535No Hit
GCTTTGGGGACCTATCTAAACTTTTATTTAAATTTCTGTTTAAACTTCTATT1850.10818460386891535No Hit
AATTAAGGGATGGTAACTGTACATTTTGTGCTAAAATGCATATTTATATGTG1850.10818460386891535No Hit
CTGATGTTGGGGGGATGCTTCATAAGTCATGATGTTTTTTAATATATAAATG1850.10818460386891535No Hit
TATTGTGGGAGAAAGTTTGCTGAAAGCAAAGGGCTAAGCTTATGAAGGAATG1830.107015040583846No Hit
CTAGTAGGGATTGCCCTCTGCCCAGCTGACAGTGGCATCCCCAGGGAAGGGG1830.107015040583846No Hit
AATGGTGGGAATTCCGTCAGATGTGGGCCGAATTTGAATGGGAAAACAAAGT1820.10643025894131132No Hit
GCAAAAGGGGAAACCCCACCTCTACTAAAAATACGAAAATTAACTGGGCATG1810.10584547729877664No Hit
TATTTAGGGAGAGTAACTGGTGGGTTACTAGATCGACTGGATGACAGCCCTG1800.10526069565624195No Hit
TTATTAGGGAAAACCAAATGGAGAAGAACAAAAAGATGAAGGCAACTCGAAA1800.10526069565624195No Hit
TCACAGGGATGGAAGAAACTGCTCTCAGGGACATGGATGGAAAATATTAGTG1790.1046759140137073No Hit
TTTAGCGGGAAGGATAAAATTGGAGAAAAGGAAATTAAATATAGCCAGTAGT1790.1046759140137073No Hit
GGTGCGGGAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTCGTAGT1780.10409113237117261No Hit
TTACGTCGGGGAAGAAACAAAAGATAGACTCACTGAAAAGTTACAAGAAACC1760.10292156908610324No Hit
GGTCGCGGGGACCAGAATCTAAGAAAGATGAAAATTAATTTCAAATACTGAA1760.10292156908610324No Hit
AATTAGGGATCTTTGAGTCTGTGTAACCAGGAGTAATTGGGAGATGTTTCTG1750.10233678744356858No Hit
CCTTCTGGGATTTCAATATGCCTTAATCATCAAAATATGTTTGAATTATCGA1740.1017520058010339No Hit
AAGAAGGGGAGTGAAAAATGTAGCTGCTGACTGTTTCTGAACTGGATATCGC1730.10116722415849921No Hit
CAGCCTGGGAAGTTTGTTTATTCCTGTAGCCTAAAAATCTATGCTTCAGGAT1730.10116722415849921No Hit
CTAGCTGGGATCTCTGTGGTTTTTGTCCTGCGGAATTGTTCACAAATACACG1730.10116722415849921No Hit
TAGCTAGGGAACCTAAAAATAGCTGCTAAATTCTAACTTCAGGTCAGCAATG1730.10116722415849921No Hit
TACAATGGGGCCGCGCAGTTGTTGGATGAGTTAATGGGCCGGGACCGAAACC1720.10058244251596453No Hit
CAAATAGGGATTAATCATTATTTCCTGGGGAGAAATATGGAATGAATTTGAT1720.10058244251596453No Hit
TCTGCAGGGATTCATGCCCTTGACCATGTGGTTGCTTGGAGACCTGGGGTCT1720.10058244251596453No Hit
TGCGATGGGATTTGATCTTATATTTGTTGGTCTATTTCTGCACTTCCTTAAT1720.10058244251596453No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)