FastQCFastQC Report
Sat 31 Dec 2022
SRR5849594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172854
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC10600.6132342902102352No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT10270.5941430340055769RNA PCR Primer, Index 27 (95% over 22bp)
CCGCTTGGGATGTCATGGCAGAAAACTTCAGCAATAATTATTTCAATGGTAA2930.1695072141807537No Hit
TGAGACGGGAATGTTTAACTTGTAACTAATGCAGAATTGATTTTTTTAATCC2900.1677716454348757No Hit
ATAGTTGGGATTTGCCACATAGTGTTATCCTCAGGAATGAATGAGACTATAT2570.1486803892302174No Hit
AACAATGGGATCACACTGTTAGGTATTTTCAGTTTTATTTTTGAGGACTTTT2510.14520925173846136No Hit
CTTGTTGGGATGCACTAGTTGTTCACCTGAAAGATTAAGTGTACCTGCAGAG2510.14520925173846136No Hit
TTTGAAGGGTTATTCTTGAAATTAAAGTTAAACCTTGCTCTTCTAACATTAG2470.14289516007729067No Hit
AAAAGCAGGATGGGCAATATGTAAATAAATAAATGTAATATTTCATCCTTTA2460.142316637161998No Hit
CCAACAGGGCATTATTGTTCTCAACTTGGGGCTCTCCCCAACCTGGGGACCC2400.13884549967024193No Hit
CTCAAAGGGAAACTTTCTAATTTCACTGGTTTTTTTGTTTGCTTGCTCTTTA2380.13768845383965658No Hit
TTCAATGGGGATGGGAGCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTT2350.13595288509377856No Hit
CCGAGGGCTAGGGAGGGATCCGTGGACTGCTCTGGAAAGTGAGGAGTTGGGG2340.1353743621784859No Hit
GAAACAGGGGACGAAGTTCCCGTAAGCAATTGCAACCAAAACAAAAATGGAC2330.13479583926319322No Hit
CCAGGTGGGGAAACCTCTCTGGTGGGAAACGTGAAATTAAACAAAACAAAAC2310.13363879343260787No Hit
ATAGCCGGGAGAGTGAGCTCAGTGAATGCTGGGGTATCATGATGGAGTCACA2290.13248174760202253No Hit
ATTTTAGGGGTATCCAGCAGTGATCAATTGCTCTTTATAGGAAACCACCTTT2270.13132470177143718No Hit
TGGCTGGGGGATTCCTGGCCCATGGGAAGATTGCGTTTCACCTGCTCCTGAA2250.1301676559408518No Hit
TAATAGGGGCACGTATGTGGTAAAAACAGTAAAAGCAAGGTATCAAGTAAAA2240.12958913302555916No Hit
GAAAACGGGGGGCTACAAGACAAGCTGTGCATAAGGTTGATATTTAGACATC2210.12785356427968111No Hit
GTTTTGGGGAGGTAGCTATGATCATCCTCATTTTTTCTATATGAGATTTGGG2210.12785356427968111No Hit
CTTGCAGGGACCCCTCTATTGCCCTTCAATTCATGAAGTTTAAATGATCACC2190.12669651844909577No Hit
TATATCGGGAGGTTGTAGTTGCATCTTTACAGTGGTCTTTCCTTTTATTAAA2180.1261179955338031No Hit
GAAATTGGGAACTTCACCATTTCACCTATAAACCTTGATTTTTTAAAATTTT2180.1261179955338031No Hit
ACTGCAGGGAGTGGCCTCGAAGTTCTTGCCTGGTTGAGAAATTATAATGAAT2160.12496094970321774No Hit
AGTCCAGGGCAGAGGAAGAACGCAGGGAAAAGAAAAATAAAATAATGCAAGC2160.12496094970321774No Hit
CAACTGGGTCTTATATTCAACTTTGAATCAATTACTGTGCACAGGGGATGAC2110.12206833512675437No Hit
GCTTAGGGACCCGTTCTTTGCTTGGACATCCCATTTTCTTTTGTCCAGACCC2110.12206833512675437No Hit
TTCTTGGGATGGAGGAGCCTTCAATATCTTCCTATCCCTAAAGAGGAGGGAG2110.12206833512675437No Hit
CATAGAGGGATGGATATATAAGTAAGAGAATGTTCAGATTTCATAGAGGAGG2090.12091128929616902No Hit
CTTTAGGGGACTTAACACCTGTTTTCGATGAGGTTCCCGGTACTGTCACCTT2070.11975424346558367No Hit
TGGCTCGGGATAAACTTCATGGAACTACCAGACTTTTAATAGCATATATTAA2070.11975424346558367No Hit
GTACAGGGGGGAGGAGGTGGCACCAGAAATGTGTATTTGAGGATTTGATCAG2070.11975424346558367No Hit
ATAAATGGGCTCCTTTGGAAAGGGAGAGAAGAGTAATCATTGTGAAATACAC2040.11801867471970566No Hit
CATGAGGGGAGCTTGAGCCCCTTAGCACCATGTAAGGAGCACAGCATCCAAA2040.11801867471970566No Hit
TAATGTGGGATTATCTTTTGTCTTTGTCAGTATAGCCATCAAAAAGTTTTTT2040.11801867471970566No Hit
TTTAGCGGGAAGGATAAAATTGGAGAAAAGGAAATTAAATATAGCCAGTAGT2040.11801867471970566No Hit
GACACTGGGAGAAAGATTTTACTTTTGATTTTATTTGATCAATTTGTTCTTA2030.11744015180441297No Hit
GAACATGGGGGCACAGAAGAAAATGAATGTAAATTCCTTATGTTGTGCAAGA2030.11744015180441297No Hit
ATAGATGGGACACGACGCTCTTTCGATCTTGTGTTGGGATTCCAGCTTTTCC2010.11628310597382761No Hit
TACTTGGGGATTGTGCTGGCTATGACTTCAAAATGAGTCTGGAAGGGGCACA2010.11628310597382761No Hit
AATGGTGGGAATTCCGTCAGATGTGGGCCGAATTTGAATGGGAAAACAAAGT2010.11628310597382761No Hit
AGCAAAGGGAGGAGGAACTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGG2000.11570458305853494No Hit
TTAACCGGGATGTTACATATCAGCAGAAGGCAAAAACTATATAAATGAACAT2000.11570458305853494No Hit
ACCCTGGGGATCTAACTCAGGGATTCCCTTTTATAGGCGAAGAGAAAGGCAT2000.11570458305853494No Hit
TCTCTTGGGACCAATGGATAAACTAACGGAAACTGCTTTTTGACATTAAAAG2000.11570458305853494No Hit
ATCAGGGGGCGATCTGAATCTGGGACAGTCTCCGGGATCCCCAGGCCTGGAG1990.11512606014324228No Hit
GTAGCAGGGACTGGCCCCACCCTACCCTGGCCTTGCCCTGCCCTGGCCCTGC1970.11396901431265692No Hit
TTTTGTGGGATGTGAGATAGTTTTCATTTTAACTGGCATGTCATTTGCACAC1950.11281196848207156No Hit
GGCTCCGGGATACTTAACCTGCAGAAAATTACAGACAAAGAAAAAACACCAG1950.11281196848207156No Hit
GAAAAGGGGATTCTCCTTTCTACATATATACCATCATGTGCCACATAACAGC1930.11165492265148623No Hit
GTAGTTGGGCATTCCTGATGATATGGTACATCCATTTCTGTCTCAGTCATTG1930.11165492265148623No Hit
TCACAGGGATGGAAGAAACTGCTCTCAGGGACATGGATGGAAAATATTAGTG1920.11107639973619356No Hit
ACTTGCGGGGTGAGGACCAGCAAGTGTTTTCACAAATGTGTTTGACTCAAGT1900.1099193539056082No Hit
GAATAAGGGGAGGTATTGATAGCTTGCTTTATTTCTGCCTTTACGTTGACAG1900.1099193539056082No Hit
CAGCCTGGGAAGTTTGTTTATTCCTGTAGCCTAAAAATCTATGCTTCAGGAT1900.1099193539056082No Hit
CTTGGCGGGGGAAGCTAAGAAAGCTGCAAAGCAGGAAGCAAGAAGTGACAAG1900.1099193539056082No Hit
GCGTTGGGGCTGCCCTGTCTGACAGAACTCATTCTCACCAATAATAGTCTCG1890.10934083099031554No Hit
AAGATGGGGATAATTAGAGCAAGAAAGTCCTGCTTGACATTGTATTGGAGCC1890.10934083099031554No Hit
GAAAGCGGGAAGTAGAGAGCTGATAGTATGTTTTTTCTAAAGTATACAGTTT1880.10876230807502285No Hit
GTGACCGGACTCCAGAATACACCCCATATTTGTCTCGTTCTCTCCACATTCA1880.10876230807502285No Hit
GAATAAGGAGTTTTGGGGGAGTTAGTATTGATGAATGCTCTTATCATTCTCA1870.10818378515973018No Hit
ATTATAGGGAAGTTTCCCAGGACCCAAACCCAGCCTATCCTTGCAGATAATG1870.10818378515973018No Hit
CTAGTAGGGATTGCCCTCTGCCCAGCTGACAGTGGCATCCCCAGGGAAGGGG1850.10702673932914482No Hit
GCTTTGGGGACCTATCTAAACTTTTATTTAAATTTCTGTTTAAACTTCTATT1850.10702673932914482No Hit
AATAATAGGATCAGACACTGGTCTTGAAGTCTCTGAACTCTGCAAGTCACTT1850.10702673932914482No Hit
CGTAATGGGAGTTTCATCTTAGATGGCAAAGAAAAGCCAGGCTTCTCTATAC1850.10702673932914482No Hit
ATCGCCGGGGATATGAGCCATAATATCTTTCATCTTCTCTGTGCCCCGATTT1840.10644821641385216No Hit
ATTTATGGGTCACCTGTATATTTAAACTGTTCTTGGCATCTTGAAACACCTA1830.10586969349855949No Hit
ATTCAGGGGAAGCTTTGATGAGATTCTATTTTCAGTAAGTTAGTGCTTCTGG1830.10586969349855949No Hit
TCGTCGGGGGGGTGAAGGGCGGCGCGCTTAGGCAGGCGGTGGCGCGGCTGGA1830.10586969349855949No Hit
TATTGTGGGAGAAAGTTTGCTGAAAGCAAAGGGCTAAGCTTATGAAGGAATG1820.1052911705832668No Hit
ACCGCAGGGTCAGTAGCGGGAGTGTGGTAAGTGTGGAAAAGCCTTTAGATCA1820.1052911705832668No Hit
TTACGTCGGGGAAGAAACAAAAGATAGACTCACTGAAAAGTTACAAGAAACC1820.1052911705832668No Hit
ATGACTGGGATACCCTCAGAGTCTTAATTTTGCCAATCGTCAAGGTTCTGCT1820.1052911705832668No Hit
TAAGTCGGGGGGACTCCCCTGTCTTGCTCCTCGAGACCCTCCCCCATCACCG1820.1052911705832668No Hit
CATTATGGGATCCAGGCAGACGGTCCCGTGAGTCTGTCCAACAGAAAAGAGG1820.1052911705832668No Hit
ATGCAAGGGAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATGGCGCCCTG1810.10471264766797413No Hit
GGTCGCGGGGACCAGAATCTAAGAAAGATGAAAATTAATTTCAAATACTGAA1810.10471264766797413No Hit
CATGTTGGGCCAAACATCAGCAACAAGTCATGAGGTATAATGAACATATCAA1790.1035556018373888No Hit
CATATAGGGAAACATTGACCATTTAGAAAATAAATTGATGTAACACTATCTG1790.1035556018373888No Hit
ACAGTAGGGAGAGACGCCAGAGGTGCAGCTCCAGCAGCAATGGCAGTGACGG1790.1035556018373888No Hit
CTAGCTGGGATCTCTGTGGTTTTTGTCCTGCGGAATTGTTCACAAATACACG1790.1035556018373888No Hit
CGTAGAGGGTAATCAGAGCCTCTTGAGAAATTGTTACTCATTGAACTGGAGC1780.10297707892209611No Hit
TGCGATGGGATTTGATCTTATATTTGTTGGTCTATTTCTGCACTTCCTTAAT1780.10297707892209611No Hit
AACGTGGGGACAATTACTTGTTACTGAATAACTACTTATTCAAAATATCATG1780.10297707892209611No Hit
CATGGGGGTCTAGAGTGGGGTTTGAAGACAGTTCACGTGTGCCTCTGTAAGC1770.10239855600680343No Hit
GCACAGGGGATCTTATACGGGGGGAAATAATCTAAGTGAGAGGTTGGAGACT1770.10239855600680343No Hit
GCCTGAGCGGGACCAAGTTGTAGGGGAGAGACTGTGTATATATTTTTTAAAA1770.10239855600680343No Hit
TGTACAGGGATCCATACATGCATACACGTGCACATCTACATGTGTATGTATA1760.10182003309151075No Hit
AAGAAGGGGAGTGAAAAATGTAGCTGCTGACTGTTTCTGAACTGGATATCGC1760.10182003309151075No Hit
TTTGCGGGGTTTCATTTTTTAAGGACAAGATCAGGATAGCTTTGGTGAAGGT1760.10182003309151075No Hit
AATAAAGGGGCACTTATGGAGATTTTTGACTTCACAGAAATGGAAGGTAACC1760.10182003309151075No Hit
GTGTTTGGGCTGTCATACAGGTAGATCCCGAAGCACAGAGACATAAAAAAGG1750.10124151017621807No Hit
CTAGTAGGGGGGAGGGAAAGAGCACATCTTCATCCCAGGCCTCAGTAAATGG1740.10066298726092542No Hit
GCAGCAGGGATAGGCTTGACTTGCTTTAGATTGCAGTAAATAAATAAAAAAA1730.10008446434563274No Hit
TAAAACGGGATTTAACAAATACTTATATAGTTTTATGTGCCACACACTATTA1730.10008446434563274No Hit
CGTTCAGGGGAACTCAGGCTGTCTGGATCAAGAGAGCCATCTTCTAGCACCC1730.10008446434563274No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)