FastQCFastQC Report
Sat 31 Dec 2022
SRR5849590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45216
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC8641.910828025477707No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT8281.8312101910828025RNA PCR Primer, Index 27 (95% over 22bp)
AATCAAGGGACGTAGACATTTTTCCATAACCATCATGTCATTATCACACTCA1610.35606864826610046No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCT1370.30299009200283084No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1290.2852972399150743No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGT1110.24548832271762208No Hit
GTAATAGGGAGGCGTGAGCCACCACGCCCGGCCGTTGTCTTTGATTTTAAGC1070.23664189667374383No Hit
TATAATGGGATACCTAGATTGCCAAGAATTCTTTGGCCTTGAAATTCAGTAA1030.22779547062986555No Hit
AACTAGGGATGGATCACAGGGTATGAAAATGAGTAGATGAATGCTTAACAAA940.20789101203113944No Hit
TCTATAGGGTGCAGGAACAGGAGAATGACATCTTCCTGGGCTGGGAAAAAGG940.20789101203113944No Hit
TGATTAGGGGGTCGGGGCCTTTGTGTATTAAGTAACTATTTGTTAACACAGG880.194621372965322No Hit
TATATAGGGGATACCCCAGGTAAGAAGCAAGATGCAGAACGGCATCAGTGTC780.17250530785562634No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT760.1680820948336872No Hit
AATTCAGGGTAACTACTTTAGAGATTCAGTACAAAGTTCAGAGGAAAACAAA720.15923566878980894No Hit
GCTTGGGGGCATCAGGAACCAAGCACTGTTCAAACTACCCTAATAAACTTTT710.15702406227883933No Hit
GCTCTGGGGGAGACATTTGGCTTGTACGGAGAAGAGAGACAGTGTGGTGGCA700.15481245576786978No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCC690.15260084925690023No Hit
GAGCCAGGGATAGTCAGAGAACCCAGCAATACAGATCCGTCCTTGGGCAAGG680.15038924274593063No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTA680.15038924274593063No Hit
CTAATTGGGATGTAAGCTTAACCAATTGAGATGTCTGTGGTGTTGGCTCTTG680.15038924274593063No Hit
CTAGCAGGGGGCTCATGTCGGCTAACGGAGCGGTGTGGGGCCGCGTGCGAAG670.14817763623496108No Hit
GTTATAGGGAGCAAGAGCTATGATGCCGTTGTCTTCGATGTCTTGAAAGTGA670.14817763623496108No Hit
GAGAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG660.1459660297239915No Hit
TTACTGCGGGACCTTCTTATAACTGATACTCTGCTTGGAACTCATTAAATCT620.13711960368011322No Hit
CGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCG590.13048478414720452No Hit
AGGTTAGGGACTGATAAAGCAGGATAAGTCTGAACTTAAATTTCTGACTTAG590.13048478414720452No Hit
GTATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA580.12827317763623497No Hit
GATGCGGAGAGAGACATTAGGAAACTAACTGTACAAAAGAACCAAGTTTAAA570.1260615711252654No Hit
CGGCGCTGGGGTACGAAAAGGAGAATCTGGCCAGTCATGGCCAAGGTTAACA570.1260615711252654No Hit
AATCAGGGGAGATGTAAATGGAGCTAAGAGGGGTGCCTGACCAAGAGTAAAC560.12384996461429583No Hit
CGCTTCGGGACTTAAAATTTTACAACTTTATTGTCAGATAACAGATTAATTC550.12163835810332627No Hit
TCTTAAGGGGGACTCCTTGGTATTCCAATGGAGAAACAAATGATAATGGAAG540.11942675159235669No Hit
TTCAACGGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGA540.11942675159235669No Hit
TATCATGGGAGTTGGAAGTAAAGCACTCCTCAGCAAATGTAAAAGAACAGAA540.11942675159235669No Hit
GTAAATGGGGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGT540.11942675159235669No Hit
ATTTAGGGGACTAAGCAAAAGATGCGTAGTTTGCACAGAAGGTTTTGTGATA530.11721514508138713No Hit
TGTTGCGGGATACTAATATCAGAAAAATTCAATATCAGAGCAAAGAAAACTA530.11721514508138713No Hit
CGATATGGGAAGGAGGACAGCAATAACCATTTACAGCAACTCACCAATTTTG530.11721514508138713No Hit
CAAGCAGGGTCACCACTGAAATGTACTTGCCTTCTTATAAAGTCACCAGCAA520.11500353857041756No Hit
ACAGTAGGGATGCATATACAATATCTACAAAAATGATTTAAGCATTTACTTT510.11279193205944799No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGT510.11279193205944799No Hit
CTACAACGGGAATATCATCTAGGTTTTCTTTAAAATTTTATTTTGTTCTTCA500.11058032554847842No Hit
CTACAGAGGGAAGATGTAAAAGTGATCCATGTTGGACATAATTGAGTTTTTA500.11058032554847842No Hit
AAAACAGGGATCACCTACCCGTTGGTATAATTAGCCTGGGCCATATTCACTG490.10836871903750883No Hit
TTATATGGGGATGGTCATGAACCCTCCAGTCCTGTAATTTGAATTCTAAAGA490.10836871903750883No Hit
TGTAAGGGAGTTGAATTGAATGTCTATAAGGTCTAAAGGTAGAATGTGAATA490.10836871903750883No Hit
GAGCTGGGGAAGGTTGGGTCTTTCAGGCTGGTTTTGGTTTGTATTAGATCTG490.10836871903750883No Hit
TTAGGCGGGGTGTAAAATATTCTACTTCCTATAGCCACTAGCCACATGTAAC480.10615711252653928No Hit
GGTGCGGGGGAGTGAGTCTGGCAGGCTTTTTAGTTCCTGAGTTAAATTTGTA480.10615711252653928No Hit
AGCACAGGGATGATCACATTCTGAGGAACTGGAGTTTACTTCATGTAGGGAT480.10615711252653928No Hit
TTACTTGGGCGCTCTTCCGATCTGTATTTGGGACATAAAGTGTGATGGCAAC470.10394550601556972No Hit
TGGACACGGGTAAGTTGCCACCTCTGCATAAGAGCTCTCTGATCAGAAAGCA470.10394550601556972No Hit
ACATGTGGGGGCCTCCGAGCAGCTCAGGGCGCCCTTGAAAGTTCTTGGATCT470.10394550601556972No Hit
ACCAGTGGGAAAAGTTCTGTGGTGTTATGTCCAGTGTAGCTTTTTGTATTCT460.10173389950460013No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)