FastQCFastQC Report
Sat 31 Dec 2022
SRR5849588.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849588.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45931
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT8271.8005268772724303RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC7991.7395658705449477No Hit
AATCAAGGGACGTAGACATTTTTCCATAACCATCATGTCATTATCACACTCA1450.31569092769589163No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCT1280.27867888789706297No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGT1220.26561581502688814No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCTTATAC1210.26343863621519237No Hit
GTAATAGGGAGGCGTGAGCCACCACGCCCGGCCGTTGTCTTTGATTTTAAGC1110.24166684809823433No Hit
TCTATAGGGTGCAGGAACAGGAGAATGACATCTTCCTGGGCTGGGAAAAAGG1050.2286037752280595No Hit
TATAATGGGATACCTAGATTGCCAAGAATTCTTTGGCCTTGAAATTCAGTAA960.20900916592279722No Hit
GCTCTGGGGGAGACATTTGGCTTGTACGGAGAAGAGAGACAGTGTGGTGGCA940.2046548082994056No Hit
TGATTAGGGGGTCGGGGCCTTTGTGTATTAAGTAACTATTTGTTAACACAGG880.1915917354292308No Hit
AACTAGGGATGGATCACAGGGTATGAAAATGAGTAGATGAATGCTTAACAAA810.17635148374736015No Hit
TATATAGGGGATACCCCAGGTAAGAAGCAAGATGCAGAACGGCATCAGTGTC810.17635148374736015No Hit
TGTAAGGGAGTTGAATTGAATGTCTATAAGGTCTAAAGGTAGAATGTGAATA810.17635148374736015No Hit
GAGCCAGGGATAGTCAGAGAACCCAGCAATACAGATCCGTCCTTGGGCAAGG760.16546558968888117No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCCTGTCTCT710.15457969563040214No Hit
GCTTGGGGGCATCAGGAACCAAGCACTGTTCAAACTACCCTAATAAACTTTT680.14804815919531472No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTA680.14804815919531472No Hit
AGGTTAGGGACTGATAAAGCAGGATAAGTCTGAACTTAAATTTCTGACTTAG680.14804815919531472No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCC670.1458709803836189No Hit
GAGAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG640.13933944394853148No Hit
CAAGCAGGGTCACCACTGAAATGTACTTGCCTTCTTATAAAGTCACCAGCAA630.1371622651368357No Hit
CGATATGGGAAGGAGGACAGCAATAACCATTTACAGCAACTCACCAATTTTG620.1349850863251399No Hit
ACAGTAGGGATGCATATACAATATCTACAAAAATGATTTAAGCATTTACTTT610.13280790751344407No Hit
TTACTTGGGCGCTCTTCCGATCTGTATTTGGGACATAAAGTGTGATGGCAAC600.13063072870174827No Hit
CTAGCAGGGGGCTCATGTCGGCTAACGGAGCGGTGTGGGGCCGCGTGCGAAG590.12845354989005248No Hit
TTCAACGGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGA580.12627637107835665No Hit
AATCAGGGGAGATGTAAATGGAGCTAAGAGGGGTGCCTGACCAAGAGTAAAC570.12409919226666086No Hit
GATGCGGAGAGAGACATTAGGAAACTAACTGTACAAAAGAACCAAGTTTAAA570.12409919226666086No Hit
GCATCGGGAGCAAAATGTGGAAGCAACCACAATGTCCTTCATTAGGTGAATA570.12409919226666086No Hit
GTATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA560.12192201345496505No Hit
CGCTTCGGGACTTAAAATTTTACAACTTTATTGTCAGATAACAGATTAATTC560.12192201345496505No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGT560.12192201345496505No Hit
GAGCTGGGGAAGGTTGGGTCTTTCAGGCTGGTTTTGGTTTGTATTAGATCTG560.12192201345496505No Hit
ACTGTGGGGACCTGTATTTTCAAAATTTAAGCCTGACAAAAAAACCCCTTTA550.11974483464326925No Hit
TACTAAGGGGATTATAATTAGCATAAATTATTTGCACCTTAATAAGTCCTCA540.11756765583157344No Hit
TTACTGCGGGACCTTCTTATAACTGATACTCTGCTTGGAACTCATTAAATCT530.11539047701987763No Hit
AGTAAAGGGGTTGCAAGTTTTTTCTCCAGTTGTCAATGTTATGTTCATAGTT520.11321329820818184No Hit
GTTATAGGGAGCAAGAGCTATGATGCCGTTGTCTTCGATGTCTTGAAAGTGA520.11321329820818184No Hit
CTACAGAGGGAAGATGTAAAAGTGATCCATGTTGGACATAATTGAGTTTTTA520.11321329820818184No Hit
GCATAAGGGTACTGGGGCAGGGCAGCAGGCTTTTGTGTTCTCTAAAAATGTT520.11321329820818184No Hit
AGCAGGGAGACTACAGAGTTTGCCCAAATCAACTGTAAATGACACTTGTGTA520.11321329820818184No Hit
CGGCGCTGGGGTACGAAAAGGAGAATCTGGCCAGTCATGGCCAAGGTTAACA510.11103611939648603No Hit
TAGTAGGGATGAACCAGATTTGGATATCGAAATAGATTTTCCCAGAGGTACT510.11103611939648603No Hit
TGGCCTGGAGATCTCCCAGAAAGAAAATCAACAAAGAGACATCAGACTTAAC510.11103611939648603No Hit
AAAACAGGGATCACCTACCCGTTGGTATAATTAGCCTGGGCCATATTCACTG500.10885894058479022No Hit
AATTCAGGGTAACTACTTTAGAGATTCAGTACAAAGTTCAGAGGAAAACAAA500.10885894058479022No Hit
ATAGCCGGGCAGTCCAGGAGGCCTGGGCTGAGGACGTGGACCTCCGGGTGAA500.10885894058479022No Hit
GTGATACGGGAGAGTCTTTAGTTTAACACAGAAAGGGGTGGGGGAACTCACA500.10885894058479022No Hit
CAACGGGGTTCACACCTAAAACAAAATGAAACCAAATTCTTACTGTAAATGA500.10885894058479022No Hit
CGGAGTGGGGACTTTGAAATTCAGCTTATGTAACATCTTCAACGAGAAACAG490.10668176177309442No Hit
TGGACACGGGTAAGTTGCCACCTCTGCATAAGAGCTCTCTGATCAGAAAGCA490.10668176177309442No Hit
GAAGCTGGGAGTGAGGAGTAGAATCAGACGTTGGTCCCTTACCTTTTGACTC480.10450458296139861No Hit
TTTTAGGGGACAACAAGGCATCCTTTTGGCTAATTTTGCTTCATTTAACGGT480.10450458296139861No Hit
CATAATGGGATAGTTCCAAAGCTAAATATATAAAAAAGGTATATTCTGAGAA470.1023274041497028No Hit
ATGTTGGGGCTGTTTTGCTCTGAACATCGCCCAAAGATCAAAAGTGAACACC470.1023274041497028No Hit
GTGACAGGGGTTAGAGAAAATATTAGTGTATAGAGCCCCAAGTACTGTTGGA470.1023274041497028No Hit
TGAACGGGGGATTGCTAGGTTGCATAGAGGACAAACATGGAAATTAATGAAG470.1023274041497028No Hit
CGGTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCT470.1023274041497028No Hit
ATAGACGGGATTTAGTTAGATCTTGAGTGAAGAGATACTGTGTTAGCTGACT470.1023274041497028No Hit
AAACTCGGGATAGCACAAATACGTTTTTAAAAGCAAAGTCTGACGTTTTGCC460.10015022533800699No Hit
CAGCATGGGATGGCTGAAAAACTTGGCATTCATCCCAGCAGCTGCCATGGAT460.10015022533800699No Hit
AATTCCGGGGGATTCATTGATTATTTTAAAGGGTTTTTCGTGTCTCTATCTT460.10015022533800699No Hit
CTATTATGGGGGGGTAGAAACCCCAAATGTCCAAAAGCCCTTTTCTGGTGGG460.10015022533800699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)