FastQCFastQC Report
Sat 31 Dec 2022
SRR5849583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266156
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGCCGTCT16950.6368445573272817RNA PCR Primer, Index 27 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGTCCTATCTCGTATGC13450.5053427313304979No Hit
CGTACAGGGAACTACCCATAGTGATTCTGAAAGGTCCCTGGGGCACAAGTTC2910.10933437532875458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)