FastQCFastQC Report
Sat 31 Dec 2022
SRR5849569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95187
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT8790.9234454284723754RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC8650.9087375376889701No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1590.16703961675438875No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1430.15023059871621125No Hit
CTTAGCGGGACCTAGATTAGTTGTTTCATACCTGGATTTTGGCATCATGCAG1360.1428766533245086No Hit
TGACTGGGGGTGTGGATGGATGAATGAGCAGTGGGAGTGCAGCGCCAGGTGA1320.13867439881496424No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCT1150.12081481714940065No Hit
TGCAGCGGGAAGCCTCCCATTTTGTTAGCGTCAGCTAGGCCAATAGGAACAG1140.11976425352201456No Hit
ATGTCGGGGCATGAGAAGGCGCTGCCTAGCTATGGGACTGGCTGCAGCGAGA1110.11661256263985628No Hit
ATTATGGGAAATGAGAATAGGGTAATTGGTCAATTTCATGGGGGAAAAATGG1090.1145114353850841No Hit
ATGCCGGGGAACTATAATTAATATGTTAAAGGCTCAAATGGGAAAAGTAGAA1060.11135974450292582No Hit
CAGCCCTTGGTGAGAGTGAGGAGAGGAGAAGGTGAGAAACCTGAGGGCAAGA1060.11135974450292582No Hit
ATCTTAGGGATGAGACTGCTTTTTTAGGACCAAAGGATATATTTCCTTACTC1030.10820805362076755No Hit
CGGGCAGGGTCGGGAAGCTTAGTGAACTCCAAATGGGAAAACCCCAAAGAAA1030.10820805362076755No Hit
GACCGCGGGGGTGTGTGACTACTCTGTGTCTGTCTGAATGGGTCTCTGTGTG1000.10505636273860926No Hit
AGACGGGAGTGAAAATCTTTCTATGTTATTGCACTTTCCCCTAAGCCTTCTC1000.10505636273860926No Hit
CGCTGTGGGCTTAATCTATTAAAAGAAATACAAAAATCACTTCTGACTTGCC990.10400579911122317No Hit
ATATCTGGGCTCTGAATGTATCCAGGAGAGGAACTGCATAACCAAAGGGGTG960.10085410822906489No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)