FastQCFastQC Report
Sat 31 Dec 2022
SRR5849568.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849568.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94161
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT8980.9536857085205127RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC7940.8432365841484266No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1380.1465574919552681No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1360.144433470332728No Hit
CTTAGCGGGACCTAGATTAGTTGTTTCATACCTGGATTTTGGCATCATGCAG1360.144433470332728No Hit
TGCAGCGGGAAGCCTCCCATTTTGTTAGCGTCAGCTAGGCCAATAGGAACAG1220.12956531897494716No Hit
TGACTGGGGGTGTGGATGGATGAATGAGCAGTGGGAGTGCAGCGCCAGGTGA1180.12531727572986695No Hit
CGGGCAGGGTCGGGAAGCTTAGTGAACTCCAAATGGGAAAACCCCAAAGAAA1040.11044912437208612No Hit
ATGCCGGGGAACTATAATTAATATGTTAAAGGCTCAAATGGGAAAAGTAGAA990.10513907031573583No Hit
ATCTTAGGGATGAGACTGCTTTTTTAGGACCAAAGGATATATTTCCTTACTC980.10407705950446575No Hit
ATGTCGGGGCATGAGAAGGCGCTGCCTAGCTATGGGACTGGCTGCAGCGAGA970.10301504869319571No Hit
CGCTGTGGGCTTAATCTATTAAAAGAAATACAAAAATCACTTCTGACTTGCC960.10195303788192564No Hit
CAGCCCTTGGTGAGAGTGAGGAGAGGAGAAGGTGAGAAACCTGAGGGCAAGA960.10195303788192564No Hit
AGACAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC960.10195303788192564No Hit
CGTGGAGGGCTGCATACAAATGAATCATCTCATTTACCAAAGTATGGATCAC960.10195303788192564No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)