FastQCFastQC Report
Sat 31 Dec 2022
SRR5849567.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849567.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94815
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT8390.8848810842166324RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC7670.8089437325317724No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1570.1655856140905975No Hit
CTTAGCGGGACCTAGATTAGTTGTTTCATACCTGGATTTTGGCATCATGCAG1530.16136687233032748No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1430.15082001792965247No Hit
TGACTGGGGGTGTGGATGGATGAATGAGCAGTGGGAGTGCAGCGCCAGGTGA1350.14238253440911247No Hit
ATGTCGGGGCATGAGAAGGCGCTGCCTAGCTATGGGACTGGCTGCAGCGAGA1270.13394505088857248No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCT1160.12234351104782998No Hit
ATGTGGGGGATGATTTTTAAATTTTAGTTTATATTGCCAGAAATTCTGTTTA1070.11285134208722249No Hit
TGCAGCGGGAAGCCTCCCATTTTGTTAGCGTCAGCTAGGCCAATAGGAACAG1060.11179665664715498No Hit
CAGCCCTTGGTGAGAGTGAGGAGAGGAGAAGGTGAGAAACCTGAGGGCAAGA1040.10968728576701998No Hit
AGAATAGGGATAGCGAAAGAGCCTTAGGATCTGCTGGATTAGAGCCTGTTTC990.10441385856668249No Hit
CGCTGTGGGCTTAATCTATTAAAAGAAATACAAAAATCACTTCTGACTTGCC980.10335917312661498No Hit
ACTGTCGGGCTGCTGGTTCTGTTTGAAATATGTTCTGTATTTAAAACTCAAA960.10124980224647999No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGA950.1001951168064125No Hit
CAGAATGGGAAACTGGCCTGTTTGGGAAGTTGGCTCTCTTTCTCTCCCAATT950.1001951168064125No Hit
TCTTGAGGGTTTGTCACTTTTCGAATCCATCCTTCCCCTCTGCTGGCTCTAC950.1001951168064125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)