FastQCFastQC Report
Sat 31 Dec 2022
SRR5849566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138584
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC13961.0073312936558334No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT13750.9921780292097213RNA PCR Primer, Index 23 (95% over 21bp)
TGCTAAGGGCGATCTTGTAGCGGGCAAGGCAGTACAATATTCCCCAAGCAGA2000.14431680424868673No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1960.141430468163713No Hit
TTCCATGGGATTCTTTATAACAGCTTCTGTGGAATAGGTATATAAAGGGTTT1960.141430468163713No Hit
ACTTCCGGGGATGAGTCAATTGATTGTTTGACAGTGGTTTCTAAACCTGAAT1700.12266928361138371No Hit
CTTACAGGGATGGGGTTATTACTGTGAACCAGCAAAACCATACTGTCGATAT1700.12266928361138371No Hit
GGACGCGGGTCTTAAGGCGGGACCTTATTAATCTCAGCAGTTAGCATGTTAT1580.1140102753564625No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGA1570.11328869133521909No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1540.11112393927148877No Hit
ACGTATGGGAGGAAATGCATAGTGATCTATAGCGATGGAATGCAGGTCAGTG1530.11040235525024535No Hit
TGCTAGGGGACTGATATTGCTGTAAGCATTTAAAAAATAATTACATTTTTGA1490.10751601916527159No Hit
ATTGGCGGGATTTGAGATGCTGTTGCACAAAATTCCCCTTATTTGCCAGGTG1490.10751601916527159No Hit
AGCGAAGGGGAAAGCTGTGGCAAGTATGATAGGCTAGAATTATCAAATGTTT1460.10535126710154132No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCT1450.10462968308029787No Hit
AACACAGGGAACATAGAAGCTTAGATTATGGACCTGAGACCTTACTTTTTTC1430.103186515037811No Hit
CACCAAGGGACCGGTGGCTCCACCACCAGACCAGCCCACCCCAATCCCATCC1410.10174334699532414No Hit
GACAACGGGTCGCATGACCTCTCTGACAGTCCGTATAGTGTAGTGTGCAAGT1410.10174334699532414No Hit
ACAATTGGGATTGGCTTCCATGCACTGCAATATTTATCCACAATTCTAGACA1400.10102176297408069No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)