FastQCFastQC Report
Sat 31 Dec 2022
SRR5849565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138560
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT14781.0666859122401848RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC13460.9714203233256351No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG2110.15228060046189376No Hit
TGCTAAGGGCGATCTTGTAGCGGGCAAGGCAGTACAATATTCCCCAAGCAGA2050.14795034642032331No Hit
CTTACAGGGATGGGGTTATTACTGTGAACCAGCAAAACCATACTGTCGATAT1750.12629907621247113No Hit
ACGCTAGGGCTATGTGTTCATCACAATTGATCATTTAATTCTACAGTAACTC1700.1226905311778291No Hit
ACTTCCGGGGATGAGTCAATTGATTGTTTGACAGTGGTTTCTAAACCTGAAT1680.12124711316397228No Hit
GGACGCGGGTCTTAAGGCGGGACCTTATTAATCTCAGCAGTTAGCATGTTAT1630.11763856812933024No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1580.11403002309468822No Hit
AACACAGGGAACATAGAAGCTTAGATTATGGACCTGAGACCTTACTTTTTTC1580.11403002309468822No Hit
TGCTAGGGGACTGATATTGCTGTAAGCATTTAAAAAATAATTACATTTTTGA1560.1125866050808314No Hit
CATGCTGGGTGAGAGAAATTATAAGAGCCATCACCTCCAGCTACAGATTGCA1550.11186489607390301No Hit
TCGCAGGGAATTGGAAAAAGAACTCTGTAGTTTGAGATTTGCCATACAGCAA1530.1104214780600462No Hit
ATAGTTGGGGCAACAAACATATGAAAAAAGTTCATCATCACTGGTCATTAGA1520.10969976905311778No Hit
ACGTATGGGAGGAAATGCATAGTGATCTATAGCGATGGAATGCAGGTCAGTG1520.10969976905311778No Hit
CGTGGGGGGGTCTTCCACAACGGCTGAACGAATTTAAACTTCCTCTAATAGT1510.10897806004618937No Hit
TTCCATGGGATTCTTTATAACAGCTTCTGTGGAATAGGTATATAAAGGGTTT1500.10825635103926096No Hit
ACACAGGGATATCAAAAGGTGTTAAAGAAAACTGAATTAAACCCAAAATTAT1490.10753464203233257No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGA1470.10609122401847575No Hit
GTACAAGGGAAAGGACCTGTAAAACAAAAATACAAGCTAAAAAGCGAAAACA1470.10609122401847575No Hit
GTAGATGGGAGAAATGGTGAGAGCCTTGAAGATACACGAGGATGAAGTAGAG1470.10609122401847575No Hit
GTTCCGGGAAGGCCAGGCTGGCCAACAAAGTGAGACCCCCACCCCCAATCCT1430.10320438799076212No Hit
ATAGCTGGGGCCATGTAAGTTGTGCCTTTTGCCTTTCACCTTCCGCCATGTT1410.10176096997690531No Hit
AGAGCGGGAAAGAGAGAGGGAACGAGACAGGGAAAAAGAACGGGAACGAGAA1400.10103926096997691No Hit
TAACCTGGGAAACCACAGCAACAGCTTTTGGATGTGTAGATGAAGATCAGAA1400.10103926096997691No Hit
ATTGGCGGGATTTGAGATGCTGTTGCACAAAATTCCCCTTATTTGCCAGGTG1400.10103926096997691No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)