FastQCFastQC Report
Sat 31 Dec 2022
SRR5849564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136035
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT13490.9916565589737935RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC12960.9526959973536222No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1960.14408056750101078No Hit
TGCTAAGGGCGATCTTGTAGCGGGCAAGGCAGTACAATATTCCCCAAGCAGA1790.13158378358510678No Hit
TTCCATGGGATTCTTTATAACAGCTTCTGTGGAATAGGTATATAAAGGGTTT1790.13158378358510678No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1640.12055720954166207No Hit
CATGCTGGGTGAGAGAAATTATAAGAGCCATCACCTCCAGCTACAGATTGCA1600.11761678979674349No Hit
ACTTCCGGGGATGAGTCAATTGATTGTTTGACAGTGGTTTCTAAACCTGAAT1570.11541147498805454No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCT1550.11394126511559526No Hit
CATTAGGGGGACGCTCTTCCGATTCTTGTGTAGGGACCATATTATTAAACAT1500.11026574043444702No Hit
TTTACAAGGGCAGATCCTTTTCCAATACTTATGCAAAAGCAAAAGAAAACCC1470.10806042562575807No Hit
CTTACAGGGATGGGGTTATTACTGTGAACCAGCAAAACCATACTGTCGATAT1460.10732532068952842No Hit
ACGTATGGGAGGAAATGCATAGTGATCTATAGCGATGGAATGCAGGTCAGTG1450.10659021575329879No Hit
ATAGTTGGGGCAACAAACATATGAAAAAAGTTCATCATCACTGGTCATTAGA1440.10585511081706914No Hit
TGCTAGGGGACTGATATTGCTGTAAGCATTTAAAAAATAATTACATTTTTGA1440.10585511081706914No Hit
ACGCTAGGGCTATGTGTTCATCACAATTGATCATTTAATTCTACAGTAACTC1400.10291469107215055No Hit
CACCAAGGGACCGGTGGCTCCACCACCAGACCAGCCCACCCCAATCCCATCC1390.1021795861359209No Hit
AGCGAAGGGGAAAGCTGTGGCAAGTATGATAGGCTAGAATTATCAAATGTTT1380.10144448119969127No Hit
AAAATCGGGGCAAGAACTAGTTGTTTGAGTTGATTTGCAGTGCTCTCAATGG1370.10070937626346162No Hit
GGCTGTGGGGCGGGTCATCTCCATGCAGAAGGGGGGCAACATGAAGGAGGTG1370.10070937626346162No Hit
CGTGGGGGGGTCTTCCACAACGGCTGAACGAATTTAAACTTCCTCTAATAGT1370.10070937626346162No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)