FastQCFastQC Report
Sat 31 Dec 2022
SRR5849560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123628
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC5070.4101012715566053No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT4130.3340667162778658RNA PCR Primer, Index 23 (95% over 21bp)
AAACAAGGGCGATCTGAACGAGGGACCTAACATGAGCCCAAGATGGTTCTGT2320.18765975345391012No Hit
TGCACTGGGACAGACAGTGAAGAAGAAATTAGAGAAGCATTCCGTGTGTTTG2190.17714433623450998No Hit
AAACGAGGATCCTCTGGCTGTGTCCCCACCCAAATCACATCTTGAATTGTAG2180.17633545798686381No Hit
GTTTCAGGGAGCATAAAGTGTTTATTTCAGAATATCAATACAGTTTTGTCTT2180.17633545798686381No Hit
GCCACGGGGATCCTCCTAGCAAAATATTTCTTAATAAAAACGTTAGAAATGT2180.17633545798686381No Hit
GGGACTGGGATGAGGAGAGAAATATATTGGTACTTGAGTATGTGGGCTAAAA2170.17552657973921765No Hit
TTGACCGGGAAGTTAATAATCAGAGAAATCATAACGAGATGGCTTCTAAAAA2140.17309994499627918No Hit
CACGCTGGGAGCATGGTCATGTAGTGACAGATGTAAGATTTCCAAGGGAGGT2130.17229106674863298No Hit
TTGACTGGGATGATTATAGTTGTTTATATTGTAATTTGCTATCGTTTCCCAA2020.16339340602452518No Hit
AAGCTTGGGACTAGATTAAAACTGAGTCTGTTAGAAAGAAACATACTTGAAG1990.1609667712815867No Hit
TTTTCTGGGGCTGCTAGGTAGTGTAGACTAGATGGACTAGAAAACAGTACAG1990.1609667712815867No Hit
TATCGCGGGTTGTAAATAAGAGCTCTACTTAGTTTGTTTACCATGAACATGT1960.1585401365386482No Hit
GAACAAGGGAATATCAATGTGCTTACAACTTATGGAAATGAAAAATAGTCTT1910.1544957453004174No Hit
GAACAAGGGGTGGTGAAAAACGCGGGGATCCAAAGAGCTGGATTTTTATCTC1870.1512602323098327No Hit
GTCCCAGGTTTCCACAGCTGTAGTTGAGCCCTACAACTCCATCCTCACCACC1860.15045135406218654No Hit
TAGCACCGGGACTGTAATGAATTTGGTGAGGCTTTTATCCAAAGTTCAAAAC1860.15045135406218654No Hit
CATAAAGGGAACTTTACATTTTTCATAAAGCATCAGTAAATACATATTCCAT1860.15045135406218654No Hit
GTGCCGGGCTACAGACCAAACCATTTGTATCTGGCATCACTTACTAACACAC1820.1472158410716019No Hit
CTCAACGGGATGACGAGTGCCAAACTTGATTTTCTCTAGGCACAGCTTTCTC1820.1472158410716019No Hit
GGGTGTGGGACTTGAGGTCAGGCTTTTGCCTTTTTGTCACATTGAGCCACAT1800.14559808457630957No Hit
CTACACGGGCAGGATGAACTCTCCAGGACAGCCCTACAAAGAAGGAGCTGTT1800.14559808457630957No Hit
AACGCGCGGGAGCTGGGATCTGGCCAGAAGGAGGCTTAAAGTTAGAAATTGC1790.1447892063286634No Hit
CTTTGTGCGGGCTAGCTAAACTTACTTTGAAAATATTTTTAAAAATGAGAAC1770.14317144983337107No Hit
GTTCTCGGGAAGCCGTGTGATCTGTGGTTATATAATATTTTAGTAAAATGAC1770.14317144983337107No Hit
CGCTTTGGGGATAGGCTGAGATTCACCTGGTTAGTCATGCTTGTTTTAATTT1770.14317144983337107No Hit
GTGGCCGGGACCAGATGAAATCCTAGTGGTAGCATCTTCCATGCTGTGTAAT1750.14155369333807877No Hit
AAATTCGGGAAGTTAAAGAAACTACTTAAATAAACTAAGGTGAATCCATTTG1740.14074481509043257No Hit
AAAGTCGGGAAGGTGGCAGTGAGCTATGATCATGCCGCTGCACTCTAGCGCT1730.1399359368427864No Hit
TTTTGCTGGGATGCTGCTTATTGTTTTATTCTACTGAAAAAAAATGAATTTC1720.13912705859514027No Hit
AGTTATGGGAGAAAATAAACAGGGTGATCATAATTACCACATCAAATTTTAA1710.1383181803474941No Hit
TAGAAAGGGACATGAAAAAGGTGGAGCTTTCTAGAAAAACCAACCTCTAAGG1700.13750930209984794No Hit
CATACAGGGACCTCTCCTGAGCTAAATAATTACCTTGAAGCCACTTGCCATG1690.13670042385220177No Hit
CATGTTGGGACAATAGATTGAACTTCTCTATCTCCAGCAATGGTTTACTTTA1680.1358915456045556No Hit
GTGAATGGGACAGGTAAAGAACTCTCTTGTCTTTGTAGATATCAGGTTATTT1680.1358915456045556No Hit
CTCAGCGGGAGGAGAAGTAACTGAAATTAATGAAGCTCTTGCAGAAAATCCA1680.1358915456045556No Hit
AGCGTAGGGCGATCTTGTTTGGGGCAAATTCATCAGATGCATTGGACAAAAT1680.1358915456045556No Hit
TGAAAAGGGCGATCTATGCAAGGGATTCTCTGTTTTTGGTGTAGAATAATTA1660.13427378910926327No Hit
GTGTCAGGGATTTTCTAAGACAAATGTAATGGATTAAATATCATTTAAAGTA1660.13427378910926327No Hit
ACTGCTGGGAGGCGGAGGTTGTGGTGAGCCAAGATCGTGCCACTGCACTCCA1650.1334649108616171No Hit
GAGTAGGGAAGAAAGTATTCATTTATGTAAAAGAAGTCAATTGGTATGTTAC1640.13265603261397094No Hit
GACGCGGGGATAATTAGTAATTGTGTAAACTTTAATATCAAGGGTTGTAACC1630.13184715436632477No Hit
CGTGTTGGGCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAAAGCCTACC1620.13103827611867863No Hit
TCCAGTACGGGATAGGTACATTGAGTTCCTGGTTCCTTGGAAACTAATGGTT1620.13103827611867863No Hit
TTAATTGGGAGAAATTAAAGATGTTCTTGGGCAAGGTGACTACTTCTTTCCT1610.13022939787103247No Hit
CACACTGGGAACATTTACTATCTTAAAACATACACATCTATTATGAAGTTTA1590.12861164137574013No Hit
TTTGGAGGGATTCTCAATTAAGAAAAACAGTCACATGTCACGACAAACCAAT1570.12699388488044777No Hit
AATAAGGGACCACAGTTTCTTTTCCCTGTCATCCACTGACGGATGCTTGTTG1570.12699388488044777No Hit
ATTAACGGGAATACCAATTTGGCAGAGTTTTCAAAAAAAATGATGCCACATC1560.12618500663280163No Hit
ACTTGTGGGCATTTTCATATTGAAGGACTTTTAGAATTTTCCTAGGTTTTTG1560.12618500663280163No Hit
GGATATGGGAACTTCTGATAATCATAAAGGCAATTCAACCCTTTCTGTGCCA1560.12618500663280163No Hit
TGTCTTCTCCCCAGACTGGACAAGGCACCTTCACGCAGAATAGTTTCAGGTT1560.12618500663280163No Hit
AGTTCAGAGGTTTTTAGTAGAGGGGGTTTCGCCTTGTTGCCCAGGCTGGTCC1560.12618500663280163No Hit
GCATTTGGGACTCCCAAAGCTGAAGTCTGGGATCAAGAGTTTGACCCCGTCA1560.12618500663280163No Hit
GCAATGGGGGGTTAAATCTGCTTGGTGTTCTATAATTTTCTTATACTTGGAT1560.12618500663280163No Hit
ACGGAGGGAGATTTAATACGATTAAGATATTCAGAAGTATATTTTAGAATCC1550.12537612838515547No Hit
CTTGTCGGGGAACTGGGTTCTCTGCTATACAACCTATCACAAGGCTGCAATT1540.12456725013750931No Hit
GTACTAGGGACCCTTTATTCTTAAATTTGTGTGTGTCCAACAGTTGAATTGA1530.12375837188986313No Hit
TTAGCAGGGTAGGATTTTTTTTCTCCTTGCAATATTGAGATGTCAGAGCTAA1530.12375837188986313No Hit
AGTCATGGGAGGACCTGGGCCTCTCCCCAAACCACTGAATCATAATCTGCAT1530.12375837188986313No Hit
CAGATGGGATAGAATGTTTTCCAAAAAGCTGTCCAAGCCTTTCTTTCCCTTC1530.12375837188986313No Hit
CAGCCGGGACTCCATCTTAAACCTAAAAATATTCCAGATAAATTAATAAATA1530.12375837188986313No Hit
TTTTCGGGGACATTTGACCCTAGACAAAGGAAAAGAAGTGAAACAGTTTTGA1520.12294949364221697No Hit
GCGTGCGGGGGAATCACGGGAAATATTTAGTGGTAGGATAGAAATGTTCTGT1520.12294949364221697No Hit
CGAATAGGGGACATAAAACCTAAAATAATAATGTTTTTAAAAGAAAACATTG1510.12214061539457081No Hit
ACAAATGGGAGGAACCAACATGCCTGCAACCTTCGGCCATCTGGCAGGTGGC1510.12214061539457081No Hit
GCATCAGGGATTGGCAAATAGAGTAGCTAAGAGATTGACAAGCACATTACGT1500.12133173714692465No Hit
AAGTTTGGGCATGCTCATGACCCTGCCGTGTCGTCTGAGGTACCCGTTCTTA1490.1205228588992785No Hit
TGCAGGGGAGTCCTAATAAACTATGAGAGTTATGAATGATTATAGGAGATAC1490.1205228588992785No Hit
CTATAAGGGAATCTTTACTAGTTATTGTGAGATTGCTGTGTAAGCTAATAAA1480.11971398065163232No Hit
GACCATGGGAGTGAAGACAAATGTCTTAAGAGGCTGCGATGTCTAGGTTGGG1470.11890510240398615No Hit
ATTTCAGGGGCGTACATGGCAAATCCTGCAAATATAGATTAAAAATTAAATT1470.11890510240398615No Hit
TTCTGGGGGGGGCCAGCGAGGCAAGATGGAGTTAGTGCAGGTCCTGAAACGC1470.11890510240398615No Hit
GTTCTTGGGAGTACAGCAAATTCATTCTTCATCCCATGTTAACTCCTTTTTG1460.11809622415634No Hit
CATGTTGGGCAGCTTTAAAATTTCTCTCTTTTGTACTCTGTCCCTTTATTTC1450.11728734590869383No Hit
GGCTCTGGGACTGATGTTAGAGGTTAGAATAAACATGTACTTAAGTTCTTGA1450.11728734590869383No Hit
ACATTTGGGACACTCTCTAGGTCTTCTATTTTTCCTGTTTATTTCCTTAAAC1450.11728734590869383No Hit
AGTGCTGGGATGACAGGCGTGAGCCACCGTGCGCGGCCTAAACAAGCTTTAT1440.11647846766104765No Hit
TACAAAGGATACCAAATTAAGGAAGAAAATGTTGCTGATTTGGGTTTTTCTT1440.11647846766104765No Hit
TTGTATGGGATAGTGAATGGAACTAAGAGATCAAGCCGGAGAGCTGGTAAAG1430.1156695894134015No Hit
TATCGCGGGCTTTCCCTGTGTTTACATTTGTTTTCTTAGGGTTGATTCCTAC1430.1156695894134015No Hit
AATGATGGGCAAGAACAATACATGTTTTACCCTTTCCTTTAACTAGAAGGAT1430.1156695894134015No Hit
TACAAAAGGGGTGTTTCTTTTTGTAACTTAATGTCTCTACTTTGCATATGTC1420.11486071116575533No Hit
CACGCCAGGGATTGGCTTTTTTTGACAACATACTACTAGAAACCTAGGCTGT1410.11405183291810918No Hit
CTTATGGGGGAGATGAGATGAGATGATGAGATGAAATGGAGAGTGGAAATGA1410.11405183291810918No Hit
AGTGGCGGGGGTTTTCGTTGGCCGCGCTGGGATGGCCGCCACAGCTGTAGGT1410.11405183291810918No Hit
AAGCTGGGATCATTCTTAGATGTCATATTCAAGTAAATATTTCTTCAGACTT1410.11405183291810918No Hit
CTCGTCCGGGGCTCGTCGAGTTCCTCCCCCAGCTCTTTCAAGAGACCGAGAA1390.11243407642281683No Hit
TGCACTGGGAAGACAGAAGTAAAGATGTACTCTAGAGAACAGTGTTAAGTGA1390.11243407642281683No Hit
CATACAGGGGTGTCCAGTCCCGGGGGTCTGATATGGCCATCACAGGCTGGGT1380.11162519817517068No Hit
GTTCTTGGGAGGAACTCTGGAGCCAAATGAAATTGGGTTGTGAGTTTAATGA1370.11081631992752451No Hit
TTGTCAGGGAGATTAGGAAACTACTTGAAAAAAAATTTTACTTATAAAATTG1370.11081631992752451No Hit
ATCCACGGGATCCGCCTTGCTGATGGAAGCCACACACGCTGCCCAGCCCACA1360.11000744167987833No Hit
GTTTACGGATCAATATAATTGATAAGCCTTTAGGCAAAATGATCAGCAAAGA1360.11000744167987833No Hit
TACTGTGGGGAACCAAGAAAATGAAGAAAAGGAGCAAGTTGCTAATAAAGGG1350.10919856343223218No Hit
TTTGCAGGGAGTCCAAGTTACCATAATATTTAGGACCAGCTACAGAGACGTT1350.10919856343223218No Hit
AGTTACGGGACTTTGGGCAATATGGCCATTTTCACGATATTGATTCTTCCTA1350.10919856343223218No Hit
CATTGCGGGATAGATTCTGTCAAATAAGGAAGTTTATTTTATAAATAACTCT1350.10919856343223218No Hit
TACGCCTGGGATAAACACACTTTGGTGTTGGTAACAGTATTTTAAGCATTTT1350.10919856343223218No Hit
AGATGGGCAAGACACCGCTGGCTGCTGGACACCCTCTTCACTTGTGTGTGTG1350.10919856343223218No Hit
AAGGTCCGGGGAGGCAGGGGCGAGGCGGCTGGGGACCGCGGGGCGGACGGGA1350.10919856343223218No Hit
TGCACAGGGAGATAAACCTGATTCCGTTTTATGACTTCCAGTACACAGACTA1350.10919856343223218No Hit
ATCTAAGGGATGTGAATATTGACTGTGTTGTCTTTTCTCATTTTTTACTTAC1340.10838968518458601No Hit
GTCCCTGGGATCTCAATAAACCCATCGTAAGTCCAAAATATTGGAAGTTGAA1340.10838968518458601No Hit
TACGATGGGAGGTATCCAGTGAATTTTTTGAAGAAGGATTTATGGCTGACGT1340.10838968518458601No Hit
TAAACAGGGATTTGAAGACTTTGAAAATTATGTTTTAAATTCTATTCTCTAG1320.1067719286892937No Hit
TTTGTTGGGGAGGTTAACTAAAAAAATTTTGCTATGTCTGATCTACATGTTT1320.1067719286892937No Hit
AAGTTAGGGAGAATCCAAAACCCACATCAGAACTGACTTGATGTCTTGTATG1310.10596305044164751No Hit
GCACACGGGAGGCGTAAGCCACCACACCAGCTGATGCACAATTACTTTTAAG1310.10596305044164751No Hit
CTCATTGGGATTATCTTAATTTTTAAGTCTGTAAATATATTTTAAAGTTATA1310.10596305044164751No Hit
TCAGCTCGGGGATTTTTCCTTTCTCTTTTTATTATCAATATTGTCATTTTTC1310.10596305044164751No Hit
AAGCAGCGGGATTGAGCTTATTACTCTCTATTTTGAGAAGGTAGAAGGTAAG1300.10515417219400136No Hit
TCACTGGGGAACAGATCACTCTGCAGCTCTATAAAAGAACAAAGAGTATTCC1300.10515417219400136No Hit
GTATAAGGGGGAAATATGGAAAAAGAAACAAAACATGGACTGGAAACTCTGG1300.10515417219400136No Hit
AGTAGTGGGACCAATGAGAGCCTCACACATTCAAGGATGTACTAAGCACTAC1300.10515417219400136No Hit
GTAGTAGGGGGCCTTGAGACACTTGTATTCCAAACTCTCCTGGAGGTTTCCA1300.10515417219400136No Hit
CAGTTGGGACCATTTGTTCAGATTGTCTTAATTTCTTTAATCAGTATTTTGT1290.1043452939463552No Hit
TACCATGGGGATCTGTATACTGAAAATTACAAAATGCTGATGAAAGAATAAA1290.1043452939463552No Hit
ATTTAGAGGAAGCAAGGCCAAACACAATTTACAAGTTTGGTGTAAATACTAA1290.1043452939463552No Hit
AATCAAGGGGCTGAATATTATGTTGAATAAAATAATAAAAATTAATAAAATG1290.1043452939463552No Hit
CAATTAGGGATTCATCTAATACTGTCTGCTTGAAAGTATTTTTCCATGGGAG1290.1043452939463552No Hit
TTAGTTGGGGAAAAGTTAAATATTTAAAAAAGCAAATTTTAAACACTAGAGT1280.10353641569870901No Hit
GTAAATGGGGAAGCCTGCATATACTGTGAGCTGTGATTGGGGAACACCAATG1280.10353641569870901No Hit
TCTGCTGGGGACTGGGAAGGAGAACTTATTCCTTACCAGGAAACTGGAAGCT1280.10353641569870901No Hit
TGCCTTGGGACCCACATTGTATCTGGGAAGTAACTAACTTGCTTTTGATTTT1280.10353641569870901No Hit
AAGATGGGGAAGCTTTCGTCAGTGGCAACCACTCTTAGGCAGCAGCAACTGG1280.10353641569870901No Hit
TGAATGGGATTGAGCTTCTGGACTACGCTGACTCCACTGCTGAATCCTCAAT1280.10353641569870901No Hit
GTTATAGGGTATAGGTATTGGGGCAAATACCATACATTCTCAAAGACTGCAT1270.10272753745106288No Hit
TATTAAGGGAGAGTAGTATTTGCTTTTTAGTCCATTTTGTTTTCAATACGAT1260.1019186592034167No Hit
CTCTCATCGGGCCCTTACCTAATTAGATTATGATAAATAGGTTTGTCATTTT1250.10110978095577054No Hit
TGTCAAGGGATTATAAAATAGGTTTTTGACATATAGTAAGTGTATGAGGGCT1250.10110978095577054No Hit
AGAATTGGGTTGCACCGGGAGTTCATTGCATGCTGAAGCGACATTGTTGGTC1250.10110978095577054No Hit
CATCACGGGAGACCACCTGAATCAGAAACTAGGGTTAGCAGGCCGGGCAAGG1250.10110978095577054No Hit
TTGGCTGGGCGATCTGTTACGGGGAGGACGGTAGATGGTGAGATCGCAGATG1250.10110978095577054No Hit
ATATCCGGGAGGGTCTTGCTGTGTCATCCAGGCTGGAGTACAATGGTGCAGT1250.10110978095577054No Hit
ACAGCCGGGAATTATTCTCATTCACTCTAACGGAAACTACCACTAGTACAGA1240.10030090270812438No Hit
GGTCCGGGGATCTCGCTAGGGGATAGGAACATAATATTTTGATTTATGAAAC1240.10030090270812438No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)