FastQCFastQC Report
Sat 31 Dec 2022
SRR5849559.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849559.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121852
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC5250.4308505399993435No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT3970.32580507500902733RNA PCR Primer, Index 23 (95% over 21bp)
TTGACTGGGATGATTATAGTTGTTTATATTGTAATTTGCTATCGTTTCCCAA2340.19203624068542166No Hit
TGCACTGGGACAGACAGTGAAGAAGAAATTAGAGAAGCATTCCGTGTGTTTG2240.1838295637330532No Hit
CACGCTGGGAGCATGGTCATGTAGTGACAGATGTAAGATTTCCAAGGGAGGT2180.17890555756163215No Hit
GCCACGGGGATCCTCCTAGCAAAATATTTCTTAATAAAAACGTTAGAAATGT2160.17726422217115845No Hit
AAACGAGGATCCTCTGGCTGTGTCCCCACCCAAATCACATCTTGAATTGTAG2140.17562288678068477No Hit
AAACAAGGGCGATCTGAACGAGGGACCTAACATGAGCCCAAGATGGTTCTGT2070.16987821291402685No Hit
GGGACTGGGATGAGGAGAGAAATATATTGGTACTTGAGTATGTGGGCTAAAA2030.16659554213307948No Hit
TTTTCTGGGGCTGCTAGGTAGTGTAGACTAGATGGACTAGAAAACAGTACAG1970.1616715359616584No Hit
ACGGAGGGAGATTTAATACGATTAAGATATTCAGAAGTATATTTTAGAATCC1950.1600302005711847No Hit
GTCCCAGGTTTCCACAGCTGTAGTTGAGCCCTACAACTCCATCCTCACCACC1930.15838886518071105No Hit
TAGAAAGGGACATGAAAAAGGTGGAGCTTTCTAGAAAAACCAACCTCTAAGG1910.15674752979023734No Hit
CTACACGGGCAGGATGAACTCTCCAGGACAGCCCTACAAAGAAGGAGCTGTT1910.15674752979023734No Hit
AAGCTTGGGACTAGATTAAAACTGAGTCTGTTAGAAAGAAACATACTTGAAG1910.15674752979023734No Hit
TATCGCGGGTTGTAAATAAGAGCTCTACTTAGTTTGTTTACCATGAACATGT1870.15346485900928997No Hit
AGTCATGGGAGGACCTGGGCCTCTCCCCAAACCACTGAATCATAATCTGCAT1860.15264419131405313No Hit
ACAAATGGGAGGAACCAACATGCCTGCAACCTTCGGCCATCTGGCAGGTGGC1860.15264419131405313No Hit
TTGACCGGGAAGTTAATAATCAGAGAAATCATAACGAGATGGCTTCTAAAAA1850.15182352361881626No Hit
GAACAAGGGGTGGTGAAAAACGCGGGGATCCAAAGAGCTGGATTTTTATCTC1840.15100285592357943No Hit
GGGTGTGGGACTTGAGGTCAGGCTTTTGCCTTTTTGTCACATTGAGCCACAT1800.14772018514263205No Hit
GTGCCGGGCTACAGACCAAACCATTTGTATCTGGCATCACTTACTAACACAC1800.14772018514263205No Hit
CTATAAGGGAATCTTTACTAGTTATTGTGAGATTGCTGTGTAAGCTAATAAA1800.14772018514263205No Hit
AAAGTCGGGAAGGTGGCAGTGAGCTATGATCATGCCGCTGCACTCTAGCGCT1790.1468995174473952No Hit
GTTCTCGGGAAGCCGTGTGATCTGTGGTTATATAATATTTTAGTAAAATGAC1780.14607884975215835No Hit
AGTTCAGAGGTTTTTAGTAGAGGGGGTTTCGCCTTGTTGCCCAGGCTGGTCC1770.1452581820569215No Hit
GTTTCAGGGAGCATAAAGTGTTTATTTCAGAATATCAATACAGTTTTGTCTT1770.1452581820569215No Hit
GAACAAGGGAATATCAATGTGCTTACAACTTATGGAAATGAAAAATAGTCTT1760.14443751436168467No Hit
CAGCCGGGACTCCATCTTAAACCTAAAAATATTCCAGATAAATTAATAAATA1740.14279617897121097No Hit
CGCTTTGGGGATAGGCTGAGATTCACCTGGTTAGTCATGCTTGTTTTAATTT1720.1411548435807373No Hit
AAGCAGCGGGATTGAGCTTATTACTCTCTATTTTGAGAAGGTAGAAGGTAAG1700.1395135081902636No Hit
GTGTCAGGGATTTTCTAAGACAAATGTAATGGATTAAATATCATTTAAAGTA1700.1395135081902636No Hit
GACCATGGGAGTGAAGACAAATGTCTTAAGAGGCTGCGATGTCTAGGTTGGG1690.13869284049502675No Hit
TGAATGGGATTGAGCTTCTGGACTACGCTGACTCCACTGCTGAATCCTCAAT1680.1378721727997899No Hit
AGCAATGGGAACATCAATTAGGGAACCAAAGTTGCACTATCTGGGCCCAGAT1670.13705150510455308No Hit
TAGCACCGGGACTGTAATGAATTTGGTGAGGCTTTTATCCAAAGTTCAAAAC1660.13623083740931624No Hit
CGAATAGGGGACATAAAACCTAAAATAATAATGTTTTTAAAAGAAAACATTG1660.13623083740931624No Hit
GACGCGGGGATAATTAGTAATTGTGTAAACTTTAATATCAAGGGTTGTAACC1660.13623083740931624No Hit
CTCAACGGGATGACGAGTGCCAAACTTGATTTTCTCTAGGCACAGCTTTCTC1660.13623083740931624No Hit
CATACAGGGACCTCTCCTGAGCTAAATAATTACCTTGAAGCCACTTGCCATG1650.13541016971407938No Hit
GCATTTGGGACTCCCAAAGCTGAAGTCTGGGATCAAGAGTTTGACCCCGTCA1640.13458950201884254No Hit
CGTGTTGGGCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAAAGCCTACC1630.13376883432360567No Hit
CTTTGTGCGGGCTAGCTAAACTTACTTTGAAAATATTTTTAAAAATGAGAAC1620.13294816662836884No Hit
CAGATGGGATAGAATGTTTTCCAAAAAGCTGTCCAAGCCTTTCTTTCCCTTC1620.13294816662836884No Hit
TTTGCAGGGAGTCCAAGTTACCATAATATTTAGGACCAGCTACAGAGACGTT1610.132127498933132No Hit
TACAAAGGATACCAAATTAAGGAAGAAAATGTTGCTGATTTGGGTTTTTCTT1610.132127498933132No Hit
CTTGTCGGGGAACTGGGTTCTCTGCTATACAACCTATCACAAGGCTGCAATT1610.132127498933132No Hit
TGCACTGGGAAGACAGAAGTAAAGATGTACTCTAGAGAACAGTGTTAAGTGA1610.132127498933132No Hit
TTTTCGGGGACATTTGACCCTAGACAAAGGAAAAGAAGTGAAACAGTTTTGA1600.13130683123789516No Hit
ATTAACGGGAATACCAATTTGGCAGAGTTTTCAAAAAAAATGATGCCACATC1600.13130683123789516No Hit
GCGTGCGGGGGAATCACGGGAAATATTTAGTGGTAGGATAGAAATGTTCTGT1600.13130683123789516No Hit
TGAAAAGGGCGATCTATGCAAGGGATTCTCTGTTTTTGGTGTAGAATAATTA1580.12966549584742146No Hit
GTGGCCGGGACCAGATGAAATCCTAGTGGTAGCATCTTCCATGCTGTGTAAT1580.12966549584742146No Hit
ATATATGGGAGGGTCATAACTCTCTCTCTTTCACCCAGCGGTAACCATGGAA1580.12966549584742146No Hit
TCTGCTGGGGACTGGGAAGGAGAACTTATTCCTTACCAGGAAACTGGAAGCT1570.12884482815218462No Hit
TGGCTTGGGGGTTAAGTAAACTGTGATAAATCTATTCAATAGAATATTATGC1560.12802416045694778No Hit
AGTTATGGGAGAAAATAAACAGGGTGATCATAATTACCACATCAAATTTTAA1560.12802416045694778No Hit
TCCAGTACGGGATAGGTACATTGAGTTCCTGGTTCCTTGGAAACTAATGGTT1560.12802416045694778No Hit
AACGCGCGGGAGCTGGGATCTGGCCAGAAGGAGGCTTAAAGTTAGAAATTGC1560.12802416045694778No Hit
AGCGTAGGGCGATCTTGTTTGGGGCAAATTCATCAGATGCATTGGACAAAAT1560.12802416045694778No Hit
TACAAAAGGGGTGTTTCTTTTTGTAACTTAATGTCTCTACTTTGCATATGTC1550.12720349276171092No Hit
CATGTTGGGACAATAGATTGAACTTCTCTATCTCCAGCAATGGTTTACTTTA1540.12638282506647408No Hit
GGATATGGGAACTTCTGATAATCATAAAGGCAATTCAACCCTTTCTGTGCCA1540.12638282506647408No Hit
TTAGCAGGGTAGGATTTTTTTTCTCCTTGCAATATTGAGATGTCAGAGCTAA1530.12556215737123724No Hit
GGCTCTGGGACTGATGTTAGAGGTTAGAATAAACATGTACTTAAGTTCTTGA1530.12556215737123724No Hit
GTAAATGGGGAAGCCTGCATATACTGTGAGCTGTGATTGGGGAACACCAATG1530.12556215737123724No Hit
TCAAATGGGATTTCATGATTCCATTATGTCATTTCACTTAGATGAAATGATA1530.12556215737123724No Hit
TTTGGAGGGATTCTCAATTAAGAAAAACAGTCACATGTCACGACAAACCAAT1510.12392082198076355No Hit
CTCAGCGGGAGGAGAAGTAACTGAAATTAATGAAGCTCTTGCAGAAAATCCA1510.12392082198076355No Hit
GTTATAGGGTATAGGTATTGGGGCAAATACCATACATTCTCAAAGACTGCAT1510.12392082198076355No Hit
TGCACAGGGAGATAAACCTGATTCCGTTTTATGACTTCCAGTACACAGACTA1510.12392082198076355No Hit
TGCAGGGGAGTCCTAATAAACTATGAGAGTTATGAATGATTATAGGAGATAC1500.1231001542855267No Hit
AAGTTTGGGCATGCTCATGACCCTGCCGTGTCGTCTGAGGTACCCGTTCTTA1490.12227948659028985No Hit
AAATGGGAGCAAATGGAATTAAGCAATTTATAAAAAGAATTATATATTGTGA1480.12145881889505301No Hit
TCTCTACGGGACAACGTGCTTGGTTTAGGTCGGAGCGCAAATTTTCTTGACC1480.12145881889505301No Hit
AAATTCGGGAAGTTAAAGAAACTACTTAAATAAACTAAGGTGAATCCATTTG1470.12063815119981616No Hit
GTGAATGGGACAGGTAAAGAACTCTCTTGTCTTTGTAGATATCAGGTTATTT1470.12063815119981616No Hit
CATAAAGGGAACTTTACATTTTTCATAAAGCATCAGTAAATACATATTCCAT1470.12063815119981616No Hit
ATTAGCGGGGTTAGAAATTATCTGTACAACTTGGGTGTGTATGTGTGTGTGT1470.12063815119981616No Hit
AGTGGCGGGGGTTTTCGTTGGCCGCGCTGGGATGGCCGCCACAGCTGTAGGT1450.11899681580934249No Hit
GTTCTTGGGAGGAACTCTGGAGCCAAATGAAATTGGGTTGTGAGTTTAATGA1430.11735548041886879No Hit
GCGATAGGGATCCACAGTCAGGAATTAAACTTATACCAATGAGAGCCTCACA1430.11735548041886879No Hit
TGTACCGGGATAGCTTCATAGTATCCTGAAATAGATTATTATAATGGGATAA1420.11653481272363195No Hit
ATCAGCGGGGCCCTTTTGATTTTAGAAAAATGATATCAATAATTTACATTTC1420.11653481272363195No Hit
ATTTCAGGGGCGTACATGGCAAATCCTGCAAATATAGATTAAAAATTAAATT1410.1157141450283951No Hit
GCATCAGGGATTGGCAAATAGAGTAGCTAAGAGATTGACAAGCACATTACGT1400.11489347733315824No Hit
GTAGTAGGGGGCCTTGAGACACTTGTATTCCAAACTCTCCTGGAGGTTTCCA1400.11489347733315824No Hit
TGTAAGGGAGAAGCTCTATTACTAAAAATACGTGGAGTTATCAACCAGTTGG1400.11489347733315824No Hit
AATCAAGGGGCTGAATATTATGTTGAATAAAATAATAAAAATTAATAAAATG1390.11407280963792142No Hit
TCGGTTGGGGATTTTCACTTTGTTTCTTACATGTGTACTTTTTTGTTTACTT1380.11325214194268457No Hit
CGCAAGGGACAGGGTTTTGTATTCTTTACTAAAGATATCAGAGCTCTTAATG1380.11325214194268457No Hit
AAGGTCCGGGGAGGCAGGGGCGAGGCGGCTGGGGACCGCGGGGCGGACGGGA1380.11325214194268457No Hit
GGTCCGGGGATCTCGCTAGGGGATAGGAACATAATATTTTGATTTATGAAAC1380.11325214194268457No Hit
GTACTAGGGACCCTTTATTCTTAAATTTGTGTGTGTCCAACAGTTGAATTGA1370.11243147424744773No Hit
CAGGCGGACTTCAGCTAATGATTCCGACTTGGCAGACGCTAAACTCATGGAG1370.11243147424744773No Hit
AAGCTGGGATCATTCTTAGATGTCATATTCAAGTAAATATTTCTTCAGACTT1360.11161080655221088No Hit
TTGTATGGGATAGTGAATGGAACTAAGAGATCAAGCCGGAGAGCTGGTAAAG1360.11161080655221088No Hit
GACATTGGGAGCTCAGCACGAGTTGAAATTGACTACATTAATTTCTTTCCAC1360.11161080655221088No Hit
TTACGAGGGCCAGCTTCTCCTAATTTTATACAGAGTTTCACTTATGTACTTG1350.11079013885697403No Hit
ATGTCAGGGATGTTAATCTTGTGATAAATGAAAGCACAGAGAAAAGTACATC1350.11079013885697403No Hit
ACTTGTGGGCATTTTCATATTGAAGGACTTTTAGAATTTTCCTAGGTTTTTG1340.10996947116173718No Hit
TTGGCTGGGCGATCTGTTACGGGGAGGACGGTAGATGGTGAGATCGCAGATG1340.10996947116173718No Hit
GCAATGGGGGGTTAAATCTGCTTGGTGTTCTATAATTTTCTTATACTTGGAT1340.10996947116173718No Hit
TGTCACGGGATGATACTATTGGTCTATAAAAGTTGTCCAGAATTCTTGAGCT1330.10914880346650033No Hit
ATCTAAGGGATGTGAATATTGACTGTGTTGTCTTTTCTCATTTTTTACTTAC1330.10914880346650033No Hit
ATCCACGGGATCCGCCTTGCTGATGGAAGCCACACACGCTGCCCAGCCCACA1320.1083281357712635No Hit
CTCTCATCGGGCCCTTACCTAATTAGATTATGATAAATAGGTTTGTCATTTT1320.1083281357712635No Hit
GTCAAGGGGACAGGAGGTGAGGAATATTATTCCCGTCAGGCCAGAAACTCAA1320.1083281357712635No Hit
GTTCTTGGGAGTACAGCAAATTCATTCTTCATCCCATGTTAACTCCTTTTTG1320.1083281357712635No Hit
TTCTATGGGAGATACAGCTGTACCTGATCGTCAAGGGATAAATTATTTTGTG1310.10750746807602667No Hit
TATGCACGGGGAGTCCAGGAGGAATACATTAGATAATGACACAATCAAATAT1310.10750746807602667No Hit
TGTATAGGGATTGCAATATAATCTTCAGCTACTCTCTTTCCATTGGTAAAAT1310.10750746807602667No Hit
ACTGCTGGGAGGCGGAGGTTGTGGTGAGCCAAGATCGTGCCACTGCACTCCA1300.10668680038078981No Hit
AGTAGTGGGACCAATGAGAGCCTCACACATTCAAGGATGTACTAAGCACTAC1300.10668680038078981No Hit
ACATTTGGGACACTCTCTAGGTCTTCTATTTTTCCTGTTTATTTCCTTAAAC1300.10668680038078981No Hit
CTGATTGGGAGTTTCCTCTTGGTTTCTTCAGTTAAGTCAAAACAACACGTTC1300.10668680038078981No Hit
GCTTCAGGGAGTTTTACATTTAGATATATATGATTTATTTTGAATTAATATT1300.10668680038078981No Hit
TTCTGGGGGGGGCCAGCGAGGCAAGATGGAGTTAGTGCAGGTCCTGAAACGC1300.10668680038078981No Hit
ATTTTGGGGGATGTAGAGTTGGGTGTTGATGTTAAAGGTACTTATACTTTGT1290.10586613268555296No Hit
TTGTCAGGGAGATTAGGAAACTACTTGAAAAAAAATTTTACTTATAAAATTG1290.10586613268555296No Hit
TTACCAGGGATATATGAAAACATCAACCTGTAATCAATATAAAAACATTGAT1290.10586613268555296No Hit
TTAGTTGGGGAAAAGTTAAATATTTAAAAAAGCAAATTTTAAACACTAGAGT1280.10504546499031611No Hit
TGTCAAGGGATTATAAAATAGGTTTTTGACATATAGTAAGTGTATGAGGGCT1280.10504546499031611No Hit
ATTTAGAGGAAGCAAGGCCAAACACAATTTACAAGTTTGGTGTAAATACTAA1280.10504546499031611No Hit
TTTTGCTGGGATGCTGCTTATTGTTTTATTCTACTGAAAAAAAATGAATTTC1280.10504546499031611No Hit
CACGCCAGGGATTGGCTTTTTTTGACAACATACTACTAGAAACCTAGGCTGT1270.10422479729507927No Hit
GTGCTTCAGGGAAGACTGCAAAGATCACTTTGTCACCAGCCCTGGGACCTGA1270.10422479729507927No Hit
GATGGCAGGGATACTAAGCTTTCTGTGCAGTCTCACCATTTGCATCACTGAG1270.10422479729507927No Hit
TGTCTTCTCCCCAGACTGGACAAGGCACCTTCACGCAGAATAGTTTCAGGTT1260.10340412959984244No Hit
ATATCCGGGAGGGTCTTGCTGTGTCATCCAGGCTGGAGTACAATGGTGCAGT1260.10340412959984244No Hit
GTTTACGGATCAATATAATTGATAAGCCTTTAGGCAAAATGATCAGCAAAGA1260.10340412959984244No Hit
GAGTAGGGAAGAAAGTATTCATTTATGTAAAAGAAGTCAATTGGTATGTTAC1250.1025834619046056No Hit
CACACTGGGAACATTTACTATCTTAAAACATACACATCTATTATGAAGTTTA1250.1025834619046056No Hit
TGTTATGGGATGTGCATGAATCTAACCACCCATTAATGAAGACTAGATTACA1250.1025834619046056No Hit
GCATAAGGGCATCTTTTGTATGGATAAAGTTTATGGTTTCATTTCTGAGAAT1250.1025834619046056No Hit
CAATTAGGGATTCATCTAATACTGTCTGCTTGAAAGTATTTTTCCATGGGAG1250.1025834619046056No Hit
CATTGAGGGCTCTGTTGGACATATAAGTGCATCTCTTGTTGGACATAGGCAA1240.10176279420936875No Hit
GGCACTGGGACAGGCATGAGACGCCACCCCCAGCCTACCATTGGAGATTTTA1230.1009421265141319No Hit
CTTATGGGGGAGATGAGATGAGATGATGAGATGAAATGGAGAGTGGAAATGA1230.1009421265141319No Hit
TGCCTTGGGACCCACATTGTATCTGGGAAGTAACTAACTTGCTTTTGATTTT1230.1009421265141319No Hit
GACACTGGGTACACAAAAACAGGAGTAAATAAAGGTAGTCTTTCTAAGAAAT1230.1009421265141319No Hit
CATACAGGGGTGTCCAGTCCCGGGGGTCTGATATGGCCATCACAGGCTGGGT1220.10012145881889505No Hit
TACGATGGGAGGTATCCAGTGAATTTTTTGAAGAAGGATTTATGGCTGACGT1220.10012145881889505No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)