FastQCFastQC Report
Sat 31 Dec 2022
SRR5849558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123489
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC5590.45267189790183743No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT3900.31581760318732843RNA PCR Primer, Index 23 (95% over 21bp)
GCCACGGGGATCCTCCTAGCAAAATATTTCTTAATAAAAACGTTAGAAATGT2240.1813926746511835No Hit
TGCACTGGGACAGACAGTGAAGAAGAAATTAGAGAAGCATTCCGTGTGTTTG2230.18058288592506216No Hit
AAACAAGGGCGATCTGAACGAGGGACCTAACATGAGCCCAAGATGGTTCTGT2170.175724153568334No Hit
GGGTGTGGGACTTGAGGTCAGGCTTTTGCCTTTTTGTCACATTGAGCCACAT2100.17005563248548453No Hit
TTGACTGGGATGATTATAGTTGTTTATATTGTAATTTGCTATCGTTTCCCAA2080.16843605503324183No Hit
TTGACCGGGAAGTTAATAATCAGAGAAATCATAACGAGATGGCTTCTAAAAA2060.1668164775809991No Hit
AAACGAGGATCCTCTGGCTGTGTCCCCACCCAAATCACATCTTGAATTGTAG2030.16438711140263504No Hit
TATCGCGGGTTGTAAATAAGAGCTCTACTTAGTTTGTTTACCATGAACATGT2000.161957745224271No Hit
GGGACTGGGATGAGGAGAGAAATATATTGGTACTTGAGTATGTGGGCTAAAA1990.16114795649814964No Hit
GTTCTCGGGAAGCCGTGTGATCTGTGGTTATATAATATTTTAGTAAAATGAC1980.16033816777202828No Hit
TTTTCTGGGGCTGCTAGGTAGTGTAGACTAGATGGACTAGAAAACAGTACAG1950.15790880159366422No Hit
CACGCTGGGAGCATGGTCATGTAGTGACAGATGTAAGATTTCCAAGGGAGGT1910.1546696466891788No Hit
TCCAGTACGGGATAGGTACATTGAGTTCCTGGTTCCTTGGAAACTAATGGTT1880.15224028051081473No Hit
GTGCCGGGCTACAGACCAAACCATTTGTATCTGGCATCACTTACTAACACAC1870.1514304917846934No Hit
CTCAACGGGATGACGAGTGCCAAACTTGATTTTCTCTAGGCACAGCTTTCTC1870.1514304917846934No Hit
GTACTAGGGACCCTTTATTCTTAAATTTGTGTGTGTCCAACAGTTGAATTGA1850.14981091433245067No Hit
GTGAATGGGACAGGTAAAGAACTCTCTTGTCTTTGTAGATATCAGGTTATTT1840.1490011256063293No Hit
GAACAAGGGGTGGTGAAAAACGCGGGGATCCAAAGAGCTGGATTTTTATCTC1790.14495218197572252No Hit
TTAGCAGGGTAGGATTTTTTTTCTCCTTGCAATATTGAGATGTCAGAGCTAA1750.14171302707123712No Hit
CTACACGGGCAGGATGAACTCTCCAGGACAGCCCTACAAAGAAGGAGCTGTT1740.14090323834511576No Hit
ACAAATGGGAGGAACCAACATGCCTGCAACCTTCGGCCATCTGGCAGGTGGC1740.14090323834511576No Hit
TTTTGCTGGGATGCTGCTTATTGTTTTATTCTACTGAAAAAAAATGAATTTC1730.14009344961899442No Hit
CATACAGGGACCTCTCCTGAGCTAAATAATTACCTTGAAGCCACTTGCCATG1730.14009344961899442No Hit
AAGCTGGGATCATTCTTAGATGTCATATTCAAGTAAATATTTCTTCAGACTT1720.13928366089287306No Hit
AAGCTTGGGACTAGATTAAAACTGAGTCTGTTAGAAAGAAACATACTTGAAG1720.13928366089287306No Hit
GTTTCAGGGAGCATAAAGTGTTTATTTCAGAATATCAATACAGTTTTGTCTT1720.13928366089287306No Hit
CGCTTTGGGGATAGGCTGAGATTCACCTGGTTAGTCATGCTTGTTTTAATTT1700.13766408344063033No Hit
GTCCCAGGTTTCCACAGCTGTAGTTGAGCCCTACAACTCCATCCTCACCACC1690.136854294714509No Hit
TAGCACCGGGACTGTAATGAATTTGGTGAGGCTTTTATCCAAAGTTCAAAAC1680.13604450598838763No Hit
TTTTCGGGGACATTTGACCCTAGACAAAGGAAAAGAAGTGAAACAGTTTTGA1670.13523471726226627No Hit
AAATTCGGGAAGTTAAAGAAACTACTTAAATAAACTAAGGTGAATCCATTTG1650.13361513981002354No Hit
TACAAAGGATACCAAATTAAGGAAGAAAATGTTGCTGATTTGGGTTTTTCTT1650.13361513981002354No Hit
GCATTTGGGACTCCCAAAGCTGAAGTCTGGGATCAAGAGTTTGACCCCGTCA1650.13361513981002354No Hit
AGTCATGGGAGGACCTGGGCCTCTCCCCAAACCACTGAATCATAATCTGCAT1640.1328053510839022No Hit
AGCGTAGGGCGATCTTGTTTGGGGCAAATTCATCAGATGCATTGGACAAAAT1640.1328053510839022No Hit
TGAATGGGATTGAGCTTCTGGACTACGCTGACTCCACTGCTGAATCCTCAAT1640.1328053510839022No Hit
TGCACAGGGAGATAAACCTGATTCCGTTTTATGACTTCCAGTACACAGACTA1640.1328053510839022No Hit
CGAATAGGGGACATAAAACCTAAAATAATAATGTTTTTAAAAGAAAACATTG1630.13199556235778087No Hit
AGTTCAGAGGTTTTTAGTAGAGGGGGTTTCGCCTTGTTGCCCAGGCTGGTCC1630.13199556235778087No Hit
ACGGAGGGAGATTTAATACGATTAAGATATTCAGAAGTATATTTTAGAATCC1620.1311857736316595No Hit
GAACAAGGGAATATCAATGTGCTTACAACTTATGGAAATGAAAAATAGTCTT1620.1311857736316595No Hit
CATAAAGGGAACTTTACATTTTTCATAAAGCATCAGTAAATACATATTCCAT1620.1311857736316595No Hit
GTTCTTGGGAGGAACTCTGGAGCCAAATGAAATTGGGTTGTGAGTTTAATGA1610.13037598490553814No Hit
TTTGTTGGGGAGGTTAACTAAAAAAATTTTGCTATGTCTGATCTACATGTTT1610.13037598490553814No Hit
CAGCCGGGACTCCATCTTAAACCTAAAAATATTCCAGATAAATTAATAAATA1610.13037598490553814No Hit
AATAAGGGACCACAGTTTCTTTTCCCTGTCATCCACTGACGGATGCTTGTTG1600.1295661961794168No Hit
CTTTGTGCGGGCTAGCTAAACTTACTTTGAAAATATTTTTAAAAATGAGAAC1590.12875640745329545No Hit
ATTAACGGGAATACCAATTTGGCAGAGTTTTCAAAAAAAATGATGCCACATC1590.12875640745329545No Hit
GCGTGCGGGGGAATCACGGGAAATATTTAGTGGTAGGATAGAAATGTTCTGT1580.12794661872717408No Hit
ACATTTGGGACACTCTCTAGGTCTTCTATTTTTCCTGTTTATTTCCTTAAAC1570.12713683000105272No Hit
CAGATGGGATAGAATGTTTTCCAAAAAGCTGTCCAAGCCTTTCTTTCCCTTC1560.12632704127493136No Hit
TGAAAAGGGCGATCTATGCAAGGGATTCTCTGTTTTTGGTGTAGAATAATTA1550.12551725254881002No Hit
TGCACTGGGAAGACAGAAGTAAAGATGTACTCTAGAGAACAGTGTTAAGTGA1550.12551725254881002No Hit
GCTTCAGGGAGTTTTACATTTAGATATATATGATTTATTTTGAATTAATATT1550.12551725254881002No Hit
GGCTCTGGGACTGATGTTAGAGGTTAGAATAAACATGTACTTAAGTTCTTGA1540.12470746382268866No Hit
GTGTCAGGGATTTTCTAAGACAAATGTAATGGATTAAATATCATTTAAAGTA1540.12470746382268866No Hit
TTCTGGGGGGGGCCAGCGAGGCAAGATGGAGTTAGTGCAGGTCCTGAAACGC1540.12470746382268866No Hit
CACACTGGGAACATTTACTATCTTAAAACATACACATCTATTATGAAGTTTA1530.12389767509656731No Hit
CAGTTGGGACCATTTGTTCAGATTGTCTTAATTTCTTTAATCAGTATTTTGT1530.12389767509656731No Hit
ATATCCGGGAGGGTCTTGCTGTGTCATCCAGGCTGGAGTACAATGGTGCAGT1530.12389767509656731No Hit
ATTTCAGGGGCGTACATGGCAAATCCTGCAAATATAGATTAAAAATTAAATT1520.12308788637044595No Hit
CTTATAAGGGACCGAAATTGAAAACAATATAAGCTTCCCATTTCGAAGAAGG1520.12308788637044595No Hit
AGTTATGGGAGAAAATAAACAGGGTGATCATAATTACCACATCAAATTTTAA1520.12308788637044595No Hit
GTAAATGGGGAAGCCTGCATATACTGTGAGCTGTGATTGGGGAACACCAATG1520.12308788637044595No Hit
AAGGTCCGGGGAGGCAGGGGCGAGGCGGCTGGGGACCGCGGGGCGGACGGGA1520.12308788637044595No Hit
CATGTTGGGCAGCTTTAAAATTTCTCTCTTTTGTACTCTGTCCCTTTATTTC1510.12227809764432461No Hit
TTTGCAGGGAGTCCAAGTTACCATAATATTTAGGACCAGCTACAGAGACGTT1510.12227809764432461No Hit
CGCAAGGGACAGGGTTTTGTATTCTTTACTAAAGATATCAGAGCTCTTAATG1510.12227809764432461No Hit
AATGATGGGCAAGAACAATACATGTTTTACCCTTTCCTTTAACTAGAAGGAT1510.12227809764432461No Hit
TTTGGAGGGATTCTCAATTAAGAAAAACAGTCACATGTCACGACAAACCAAT1500.12146830891820325No Hit
GTGGCCGGGACCAGATGAAATCCTAGTGGTAGCATCTTCCATGCTGTGTAAT1500.12146830891820325No Hit
TAGAAAGGGACATGAAAAAGGTGGAGCTTTCTAGAAAAACCAACCTCTAAGG1490.12065852019208188No Hit
ATATATGGGAGGGTCATAACTCTCTCTCTTTCACCCAGCGGTAACCATGGAA1490.12065852019208188No Hit
CTTGTCGGGGAACTGGGTTCTCTGCTATACAACCTATCACAAGGCTGCAATT1470.11903894273983917No Hit
GCAATGGGGGGTTAAATCTGCTTGGTGTTCTATAATTTTCTTATACTTGGAT1470.11903894273983917No Hit
AAGCAGCGGGATTGAGCTTATTACTCTCTATTTTGAGAAGGTAGAAGGTAAG1460.11822915401371784No Hit
GATGAAGGGCTGTCTGTTGAGATTTCAAGTCTCTTGTCACCATCCTCACACA1460.11822915401371784No Hit
CTTATGGGGGAGATGAGATGAGATGATGAGATGAAATGGAGAGTGGAAATGA1450.11741936528759647No Hit
AAGTGGGACAGGGAAATACCTTTCCTGGGCTTGTTTTCTAGCATATCACTGA1450.11741936528759647No Hit
TGGCTTGGGGGTTAAGTAAACTGTGATAAATCTATTCAATAGAATATTATGC1440.11660957656147511No Hit
CATTGCGGGATAGATTCTGTCAAATAAGGAAGTTTATTTTATAAATAACTCT1440.11660957656147511No Hit
ACAGCCGGGAATTATTCTCATTCACTCTAACGGAAACTACCACTAGTACAGA1430.11579978783535375No Hit
GCACACGGGAGGCGTAAGCCACCACACCAGCTGATGCACAATTACTTTTAAG1430.11579978783535375No Hit
CATCCAAATAACATTGTTCCCATCCTCCCCACCCACCACATCAAAACATAAC1430.11579978783535375No Hit
GTTCTTGGGAGTACAGCAAATTCATTCTTCATCCCATGTTAACTCCTTTTTG1430.11579978783535375No Hit
GTGCTTCAGGGAAGACTGCAAAGATCACTTTGTCACCAGCCCTGGGACCTGA1430.11579978783535375No Hit
TTGGCTGGGCGATCTGTTACGGGGAGGACGGTAGATGGTGAGATCGCAGATG1430.11579978783535375No Hit
CTATAAGGGAATCTTTACTAGTTATTGTGAGATTGCTGTGTAAGCTAATAAA1420.11498999910923241No Hit
TACAAAAGGGGTGTTTCTTTTTGTAACTTAATGTCTCTACTTTGCATATGTC1410.11418021038311105No Hit
AACGCGCGGGAGCTGGGATCTGGCCAGAAGGAGGCTTAAAGTTAGAAATTGC1410.11418021038311105No Hit
TTAATTGGGAGAAATTAAAGATGTTCTTGGGCAAGGTGACTACTTCTTTCCT1410.11418021038311105No Hit
GACACTGGGTACACAAAAACAGGAGTAAATAAAGGTAGTCTTTCTAAGAAAT1410.11418021038311105No Hit
CATGTTGGGACAATAGATTGAACTTCTCTATCTCCAGCAATGGTTTACTTTA1400.1133704216569897No Hit
CTCTCATCGGGCCCTTACCTAATTAGATTATGATAAATAGGTTTGTCATTTT1400.1133704216569897No Hit
TCTCTACGGGACAACGTGCTTGGTTTAGGTCGGAGCGCAAATTTTCTTGACC1400.1133704216569897No Hit
TACGATGGGAGGTATCCAGTGAATTTTTTGAAGAAGGATTTATGGCTGACGT1400.1133704216569897No Hit
ACTGCTGGGAGGCGGAGGTTGTGGTGAGCCAAGATCGTGCCACTGCACTCCA1390.11256063293086833No Hit
TTGTATGGGATAGTGAATGGAACTAAGAGATCAAGCCGGAGAGCTGGTAAAG1390.11256063293086833No Hit
GTTATAGGGTATAGGTATTGGGGCAAATACCATACATTCTCAAAGACTGCAT1390.11256063293086833No Hit
TGTACCGGGATAGCTTCATAGTATCCTGAAATAGATTATTATAATGGGATAA1380.11175084420474697No Hit
GCGATAGGGATCCACAGTCAGGAATTAAACTTATACCAATGAGAGCCTCACA1370.11094105547862564No Hit
CAAATAGGGAGCAAAGACATTCTTGCAAGGCAGAAATTGAGAATTGAATTAT1370.11094105547862564No Hit
AGTGGCGGGGGTTTTCGTTGGCCGCGCTGGGATGGCCGCCACAGCTGTAGGT1360.11013126675250427No Hit
TGTAAGGGAGAAGCTCTATTACTAAAAATACGTGGAGTTATCAACCAGTTGG1360.11013126675250427No Hit
TACCTTGGGAGTGACTGGATGAAAAACCTCATCTCCCCTGACTTAGGAGTTG1350.10932147802638291No Hit
ATCCACGGGATCCGCCTTGCTGATGGAAGCCACACACGCTGCCCAGCCCACA1350.10932147802638291No Hit
TGACGCTGGGATTTTAAGGTACTGGAGTTTAGCACTTCATTGTATCTTTTGA1350.10932147802638291No Hit
TGTCTTCTCCCCAGACTGGACAAGGCACCTTCACGCAGAATAGTTTCAGGTT1350.10932147802638291No Hit
TCGGTTGGGGATTTTCACTTTGTTTCTTACATGTGTACTTTTTTGTTTACTT1340.10851168930026156No Hit
TCTGCTGGGGACTGGGAAGGAGAACTTATTCCTTACCAGGAAACTGGAAGCT1340.10851168930026156No Hit
CGTGTTGGGCTGGCCACATATGCCCCTGTCATCTCTGCTGAGAAAGCCTACC1330.10770190057414021No Hit
TAAACAGGGATTTGAAGACTTTGAAAATTATGTTTTAAATTCTATTCTCTAG1330.10770190057414021No Hit
GACGCGGGGATAATTAGTAATTGTGTAAACTTTAATATCAAGGGTTGTAACC1330.10770190057414021No Hit
TGTCAAGGGATTATAAAATAGGTTTTTGACATATAGTAAGTGTATGAGGGCT1320.10689211184801886No Hit
AGATGGGCAAGACACCGCTGGCTGCTGGACACCCTCTTCACTTGTGTGTGTG1320.10689211184801886No Hit
GCATCAGGGATTGGCAAATAGAGTAGCTAAGAGATTGACAAGCACATTACGT1310.1060823231218975No Hit
TATTAAGGGAGAGTAGTATTTGCTTTTTAGTCCATTTTGTTTTCAATACGAT1310.1060823231218975No Hit
ACTTGTGGGCATTTTCATATTGAAGGACTTTTAGAATTTTCCTAGGTTTTTG1300.10527253439577614No Hit
TAAAGGGGGATCTCTGAAATCTGTAAGCCATATTTCAGCTATTCTGGTTTTT1290.10446274566965477No Hit
AGAATTGGGTTGCACCGGGAGTTCATTGCATGCTGAAGCGACATTGTTGGTC1290.10446274566965477No Hit
CTCAGCGGGAGGAGAAGTAACTGAAATTAATGAAGCTCTTGCAGAAAATCCA1290.10446274566965477No Hit
TTTAGTGGGAGAGTGTTTGATTTTCTTGAATAAAACTAGGGAGGGGAATTTT1290.10446274566965477No Hit
GGTCCGGGGATCTCGCTAGGGGATAGGAACATAATATTTTGATTTATGAAAC1290.10446274566965477No Hit
GTTTTGGGGGTCCTTTGTTCTCTTCAAAATGTTCCAAGTTAAGGAGTGAAGC1290.10446274566965477No Hit
TATGTAGGGAGTGGAATAGATTCTCCACGGACACAGAGTCTGGGATCTAATA1280.10365295694353344No Hit
GGATATGGGAACTTCTGATAATCATAAAGGCAATTCAACCCTTTCTGTGCCA1280.10365295694353344No Hit
TTCTATGGGAGATACAGCTGTACCTGATCGTCAAGGGATAAATTATTTTGTG1270.10284316821741207No Hit
GAGTAGGGAAGAAAGTATTCATTTATGTAAAAGAAGTCAATTGGTATGTTAC1270.10284316821741207No Hit
GACCATGGGAGTGAAGACAAATGTCTTAAGAGGCTGCGATGTCTAGGTTGGG1270.10284316821741207No Hit
TTAATCGGGGGAGATATCTTTTTATGGCTTAGCACAATGCTGCTCAAAGCGC1270.10284316821741207No Hit
CGATTAGGGGGAACAGAAATAATATACTGCAGCCTCACCAAAATAAAAGTTA1270.10284316821741207No Hit
AATCAAGGGGCTGAATATTATGTTGAATAAAATAATAAAAATTAATAAAATG1270.10284316821741207No Hit
CATTGAGGGCTCTGTTGGACATATAAGTGCATCTCTTGTTGGACATAGGCAA1270.10284316821741207No Hit
ATTAGCGGGGTTAGAAATTATCTGTACAACTTGGGTGTGTATGTGTGTGTGT1270.10284316821741207No Hit
CTGCTCGGCTGACCATGCATGCTTTTGTGGCAGAGTGGGATTTTCATGAGCA1260.10203337949129072No Hit
GACATTGGGAGCTCAGCACGAGTTGAAATTGACTACATTAATTTCTTTCCAC1260.10203337949129072No Hit
GAAGGAGGGATGGAATTAATATATTATAAAGATGATAACAGGGCAGAAAAAT1260.10203337949129072No Hit
GTTCCTGGGATATTTAACTTTGTATAGCCCATGTACCTACCTTGTATAGAAA1260.10203337949129072No Hit
TATGGTGGGAGTGAATGTCAACCACCGCAGAGTCTGAGGCTCATTTCCACTA1260.10203337949129072No Hit
AGTGCTGGGATGACAGGCGTGAGCCACCGTGCGCGGCCTAAACAAGCTTTAT1250.10122359076516936No Hit
TATCGCGGGCTTTCCCTGTGTTTACATTTGTTTTCTTAGGGTTGATTCCTAC1250.10122359076516936No Hit
CTTTTTGGGGTGCTAGAGTAAGAATTTAATACTTGACCTCTCTGGCTTCCAG1250.10122359076516936No Hit
ATTTTGGGGGATGTAGAGTTGGGTGTTGATGTTAAAGGTACTTATACTTTGT1250.10122359076516936No Hit
CACGCCAGGGATTGGCTTTTTTTGACAACATACTACTAGAAACCTAGGCTGT1240.10041380203904803No Hit
TTACCAGGGATATATGAAAACATCAACCTGTAATCAATATAAAAACATTGAT1240.10041380203904803No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)