FastQCFastQC Report
Sat 31 Dec 2022
SRR5849553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105004
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT9570.9113938516627939RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC8140.7752085634832958No Hit
TATTACGGGAAGTGAGAACACGTGGGATTTGGTTTTCTGTTCCTGTGTTAGT1590.15142280294084035No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1300.12380480743590722No Hit
TCAAGGGGGCTCTTGGCGTCAGCGAAGGCTCTGCTGCCTGCAGGGCAGAGAG1270.1209477734181555No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1080.10285322463906137No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)