FastQCFastQC Report
Sat 31 Dec 2022
SRR5849542.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849542.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101310
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT10341.020629750271444RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC10010.988056460369164No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1800.17767249037607344No Hit
CCTAGGGGTTGAATGGATAAAACAAATCTTTTTATCCAAAGATAAAGGGTCC1370.13522850656401145No Hit
CAAATGGGGATTACTATCATAAGGAGAGAAGAGCTGTCAAGTTTCAGAAAAC1360.13424143717303327No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGA1280.12634488204520777No Hit
AACGCTGGGATTTACCTCTGTTTCCAAACTCTGCTCTAGCTTCTCCAAAGCT1180.11647418813542593No Hit
GCATAGGGGTTCTGTTTTCTCTTTTTAAATATTTTATAACTTTTAAATGGAA1170.11548711874444773No Hit
TCTCAGGGGATGTATCAATTGATACCAACATTCATCTAATTAGTGCACACGC1110.10956470239857861No Hit
CACTTTGGGGAAAAGGAAGTAGGGCATAATGCCATAGAAACAAGGCAAATAT1100.10857763300760044No Hit
GTATACGGGTGCGGGCTGCTGGCGCTCGTGCCACTCTGCTGGTTCGCCAACA1090.10759056361662224No Hit
TTAGTCCGGGGATAAGGACACAGATGTGAATGGCAAAGTGATCTGTTTTATT1080.10660349422564407No Hit
TAACCAGGGGTATCACAACTAATGCATCATGGAGCCTCACCAAACACCACCA1030.10166814727075313No Hit
CTCTCAGGGATTCCTCTGTGTTTAAGAGACTGGGTTCTCACACCTGATGTAT1030.10166814727075313No Hit
ATCTTTGGGGCTCTGCTGATGGCGACGCTTGGCCGACTGAAGCGGTTCGCTT1030.10166814727075313No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)