FastQCFastQC Report
Sat 31 Dec 2022
SRR5849541.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849541.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101053
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT10791.0677565238043403RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC10211.0103608997258864No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1640.1622910749804558No Hit
CCTAGGGGTTGAATGGATAAAACAAATCTTTTTATCCAAAGATAAAGGGTCC1460.1444786399216253No Hit
TTAGTCCGGGGATAAGGACACAGATGTGAATGGCAAAGTGATCTGTTTTATT1370.13557242239221No Hit
CAAATGGGGATTACTATCATAAGGAGAGAAGAGCTGTCAAGTTTCAGAAAAC1350.13359326294122886No Hit
AACGCTGGGATTTACCTCTGTTTCCAAACTCTGCTCTAGCTTCTCCAAAGCT1290.12765578458828536No Hit
CTCTCAGGGATTCCTCTGTGTTTAAGAGACTGGGTTCTCACACCTGATGTAT1240.12270788596083243No Hit
TAACCAGGGGTATCACAACTAATGCATCATGGAGCCTCACCAAACACCACCA1230.12171830623534186No Hit
TTTACGGGAAGCAATGGACCCTCCAGCAACAACAGAAACTGTCCTTCTCCCA1170.11578082788239834No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1110.10984334952945485No Hit
ATCTTTGGGGCTCTGCTGATGGCGACGCTTGGCCGACTGAAGCGGTTCGCTT1110.10984334952945485No Hit
GCTAAAGGGATAAGTGGTAGCTGTATCTAGTGAAGTGTCTGTCAGTAAGTGA1090.10786419007847367No Hit
AATAAAGGGATCCACAGGGTTTCAAGAGACCATTTAGTGATTCCTCACATTC1070.1058850306274925No Hit
GAACGTGGGAGCTAAGTTACAGGCGTCAACCACCACACTCGGCCCTCCTTGC1070.1058850306274925No Hit
GTAAATGGGATGAAAAGTTCTGCTAGAAGGAGAGCCACTCAGTATATTTTGA1040.10291629145102076No Hit
TTGTCAGGGCATGGACTTTGGAGAGAAGTTCTCTCGGGATTTCTTGGATTCC1030.10192671172553017No Hit
ATACTTGGGGATCTACATCCGGGCTGATGTTCGCCGACCGTTGACTATTCTC1030.10192671172553017No Hit
CAGGCAGGGATAATCAGACTTTAGACAATCCTAAGCAAAAGAAACAAAGTCA1030.10192671172553017No Hit
ACTAACGGGGTTACTGATTTTTAGCTTAATTACACTGTGGTCAGAGAACATT1030.10192671172553017No Hit
GCTCCCGGGAAGACAAGAAACACAAGAATGGAATCAACAGTTCTGGGCAAAC1020.10093713200003958No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)