FastQCFastQC Report
Sat 31 Dec 2022
SRR5849540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98430
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT10051.0210301737275223RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC9450.9600731484303565No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1790.18185512546987706No Hit
AACGCTGGGATTTACCTCTGTTTCCAAACTCTGCTCTAGCTTCTCCAAAGCT1300.13207355481052527No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1250.12699380270242813No Hit
CAAATGGGGATTACTATCATAAGGAGAGAAGAGCTGTCAAGTTTCAGAAAAC1190.12089810017271156No Hit
CCTAGGGGTTGAATGGATAAAACAAATCTTTTTATCCAAAGATAAAGGGTCC1180.11988214975109214No Hit
GGCTTAGGGCTACCTGTAACAATTTTGTTTTCTACTGTTAATTACATTGTGT1170.11886619932947272No Hit
TAACCAGGGGTATCACAACTAATGCATCATGGAGCCTCACCAAACACCACCA1160.1178502489078533No Hit
TTGCAGGGGATCTCATTTAATCCTCTCAACAACCATATGACATAGACCCTGC1080.1097226455348979No Hit
CTAACAAAGGGCACGAAATTGCCATATAATATCTTGTCTTTTTTGGGGGTGT1060.10769074469165905No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGA1040.10565884384842018No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)