FastQCFastQC Report
Sat 31 Dec 2022
SRR5849530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216317
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC10640.4918707267574902No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT10190.47106792346417525RNA PCR Primer, Index 23 (95% over 21bp)
GTCTTAGGGAAAGATAATTACTTTTGAATAGTTAGGTTTGAAGTTTTCAGAG2330.10771229260760828No Hit
AAGCTGGGGCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACAGAG2320.10725000808997906No Hit
GATAACGGGACTTATACCTGCTATCAGCAGCTAGAAAACATTTTTTTTTAAA2310.10678772357234984No Hit
AAGGTTGGGAGTAGAAAATATAGAAAATATAGATGAGCAAAAAGTTGAAGAT2280.10540087001946219No Hit
AAGTTGGGGAGCTCAGGTGGTGATGGCAGGTGGACTCTGGCTTCTAATCCCA2260.10447630098420373No Hit
TTTGTCGGGCATGCAGGGTTGTGTGTTTGTGTGTATTATGAGCAGTAGTCAA2250.10401401646657452No Hit
CTGCAGGGGAGGGACAAGAAGTTAGTCCTAAAGTGTACTTCATGAAGCAGAC2250.10401401646657452No Hit
TTGACTGGGATACACAAAATTATTACTGCTTATCTTTGCTCTTTTCCTTGTT2200.10170259387842842No Hit
GCATTTGGGTAGCTCTTTTCAGTTTCTACAGTTCCAAGAATTACACATGACA2190.10124030936079921No Hit
CTACACGGGATGAATAACAAACATAAAAGTTACATGTTTTTTAGAGTAAGAA2180.10077802484316997No Hit
CATTGAGGGGCAGTTCTCTACCTGTGTTTGCTCTCACCTCCATTTTCAGGTA2170.10031574032554075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)