FastQCFastQC Report
Sat 31 Dec 2022
SRR5849529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217662
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC10900.5007764331853976No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT10330.47458904172524374RNA PCR Primer, Index 23 (95% over 21bp)
AAGCTGGGGCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACAGAG2560.11761354761051539No Hit
TCAATCGGGTTGCTAGCAAGGCTGAGCACTTTTCCATGTGATGATTTACTAG2470.1134786963273332No Hit
TAGCAGGGGCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAAACCC2440.11210041256627247No Hit
GCACAGGGGAACCTTTCTAAGGCCATTTCTTCAGCTGTAGACTAGTGGAAAT2440.11210041256627247No Hit
GATTGTGGGTATTAATTTTGGGTTTGGTCTGCTCTTGCTTTTCTAGTTAAGA2420.11118155672556532No Hit
AAGTTGGGGAGCTCAGGTGGTGATGGCAGGTGGACTCTGGCTTCTAATCCCA2360.10842498920344387No Hit
GTTACAGGGTCTTGCATGTTTGTATCTAATTTCTGAACGAATGAGCACACTT2340.10750613336273672No Hit
GCAACTGGGAACTTTGCCTCGTTCATAATCTTAATGAGAAAGCTACAAGTTT2320.10658727752202958No Hit
GTCGCTGGGATGCTCCTGAATGACCAGTTGGTCAATGAAGAAATTAAGAAGG2310.106127849601676No Hit
GTTTCTGGGCAAGAACTCCAGACAGTGGGAGGAAATAGAATTTCAACTGGCA2250.10337128207955455No Hit
ACCTCTGGGAAATACGATAAAGTGATAATTTCTTCATCTCTCCATCTAAGGT2250.10337128207955455No Hit
TTAGATTTGAGGAAAATAACGTTCATAGTTGTGTTTTTGTTGTTGGGTTTTT2240.10291185415920096No Hit
TTTAACGGGGATGGGCAACAACTGCCCACAGGGCAACTCCTGTGAGAAAGCC2240.10291185415920096No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)