FastQCFastQC Report
Sat 31 Dec 2022
SRR5849528.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849528.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212466
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT10470.49278472790940664RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC10290.4843127841631132No Hit
AAGTTGGGGAGCTCAGGTGGTGATGGCAGGTGGACTCTGGCTTCTAATCCCA2440.11484190411642334No Hit
GTCGCTGGGATGCTCCTGAATGACCAGTTGGTCAATGAAGAAATTAAGAAGG2390.11248858640911957No Hit
GTTACAGGGTCTTGCATGTTTGTATCTAATTTCTGAACGAATGAGCACACTT2260.10636996037012982No Hit
TCAATCGGGTTGCTAGCAAGGCTGAGCACTTTTCCATGTGATGATTTACTAG2250.10589929682866905No Hit
AAGCTGGGGCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACAGAG2230.10495796974574756No Hit
GCACAGGGGAACCTTTCTAAGGCCATTTCTTCAGCTGTAGACTAGTGGAAAT2150.10119266141406155No Hit
GCATTTGGGTAGCTCTTTTCAGTTTCTACAGTTCCAAGAATTACACATGACA2130.10025133433114004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)