FastQCFastQC Report
Sat 31 Dec 2022
SRR5849527.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849527.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173538
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC7520.433334485818668No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT7400.42641957381092327RNA PCR Primer, Index 23 (95% over 21bp)
GCTCTAGGGGGGTTCTATGGAATCTTTTCCATGTGCATGTATTTGTATCCTT2350.13541702681833373No Hit
AAGCAAGGGTCTACGAAAGGGATGACACTGACACTGCTATTTTGAAACCTGG2260.1302308428125252No Hit
TACTATGGGATTCTACATATTAATATCCCTGTGAAGTCATTACAACAGCTGT2250.12965460014521316No Hit
GCTACAGGGGGAGTCTCCCAGCTGCCAGCCGACCCCGAACCCGCACTCCCGC2220.12792587214327697No Hit
CAGCATTGATACCCAATGACAAACTATGGAGAACAAGCAAAGTATGCAGGCC2090.12043471746822022No Hit
ATTAGCGGGCAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTAT1970.11351980546047552No Hit
GAAACAGGGCTGATAACCTCTTTTGCCAACTCGCATATCTGTGTAGTTTTTC1960.11294356279316346No Hit
TTTAACGGGGTGGCACAGACTTTGAAGCCCAATTGTAAAACCAGTTCAGTTT1960.11294356279316346No Hit
GTTGCGGGAACAAAGAAGTTGTGAGTATATTATCTACATTTTAGAAAATTTT1950.11236732012585139No Hit
CTGGGGCCCGAGGGATTCAGATGCTAATGGGGACACAGGGGCCACTTGGTGT1950.11236732012585139No Hit
GTTGTTGGGACCTCCCACCCACTTGCAGTCTACTTTCTGTTTCTATGGATTT1920.11063859212391523No Hit
AAGACGGGAATCATATAATTAGTTTCAGAGTCAAATATTAAAATTATTTGGA1910.11006234945660316No Hit
TAAGCGGGGGATGTTCCTGGAAAAATTACATTTCAAGAATCTAAAACATTGA1900.1094861067892911No Hit
CACATTGGGGGAAAGAAAATCCTAGAAAGCCTATCACCATAGATCAGCTGTA1890.10890986412197905No Hit
TCTCTTGGGGTAAAACTGCTCATCTAGCCATGGAGTTTACCTTCACCTCCAA1880.108333621454667No Hit
AGCTGTGGGCTATTCTGAAGCTAAAAGAAAGCAAAGAAAAATGATGTTTACA1860.10718113612004287No Hit
AATACCGGGGCAAAAAACAAAATTTGATGTTGAGAGGCTAACATAATTAGTG1860.10718113612004287No Hit
GGTTATGGGTAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGC1850.10660489345273082No Hit
GCAACTGGGCAGGTGGTCCATCCACTTTGGCCTCCCAAAATGTTGGGATTAC1840.10602865078541876No Hit
CCAGCCTGGGCAACAGAGCGAGACTCTTGTCTCAAGAAGAAGAAAAAAAGAA1840.10602865078541876No Hit
AGCTTGGGGTCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC1840.10602865078541876No Hit
GCTTCTGGGATTGAATTACTGACTTGAGATCTTTTTTAATGTAGGTATTTAT1830.10545240811810669No Hit
GCATCCGGGATCTGAATGTAAAAGGAATCAACGGCTAAATCAAGCTTAAAGT1830.10545240811810669No Hit
AAGTAGGGGGATAAGAAAAGGAAGACAAATTTATGCTTATCTATAGTACAGA1810.10429992278348259No Hit
CAACAGGGGATCATGTGAAAGACACTCTTCAGAGTGAGCTAGTAGGCCAGCT1810.10429992278348259No Hit
CCATCCAGCTGCAGCCCATCCCCACACCTGAAATCCATCTCTTGAATCCCAG1800.10372368011617053No Hit
ATCTTCGGGATCTTACGGGGGGAGCAAAAGCAATGTGGGAGAAACCAAGTAA1790.10314743744885846No Hit
AAGCATGGGCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACT1780.1025711947815464No Hit
CTATTTGGGGGTTAAAAATAAAAGTAGTTTTTTCATGACATTCCTATCTGCC1780.1025711947815464No Hit
ATGTGCGGGCAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAG1780.1025711947815464No Hit
CGTCTAGGGGGCTTCTCCATAGGCAGAAAACTAGCGTAGTTTTTTTATTAGA1780.1025711947815464No Hit
CTCAATGGGATTCTTCTGAGAAGTTGGGGCAGAATTGGCAAGTCATTTATAT1780.1025711947815464No Hit
GAACTTGGGGGGCTCACCCTCGGGGGTCTGGCCGTGAGCGAGCACCGGCTCA1770.10199495211423434No Hit
TGTACGAGGGCTAGTAAAACAATAACCGGTAATAATTATAAAAATAATATTT1770.10199495211423434No Hit
GACGCTGGGGCAAGAACAGGGACAAGCATAATGAGCTATTTATCACTTTCTT1770.10199495211423434No Hit
CTATGCGGGACTGTCTCCAAGAAAACACAACCTGCCTTTTGGGGGTGGAGGG1770.10199495211423434No Hit
CCCTGTCTCAGTTTCCCAAAGTGCTGGGATTACAGGCATAAGCCACCATGCC1760.1014187094469223No Hit
TTCTTGGGGTGCCAAGGAATGCAGGTAGCTTCTAGAAGCTGGAAAAAACAAG1760.1014187094469223No Hit
TACACCAGGGATCCGTGATTTCATCCTTATGTGAAAAAGGGCTTAGTACTGT1760.1014187094469223No Hit
ACCTTGGGGAATGTAATGCAGTAATTAATTGCAAATACCACTCAAACTTAAA1760.1014187094469223No Hit
GCATGGGGAATGGAAGAAGTCTTCTGTTGTATAGTAACAATTGGTCAGGTTG1750.10084246677961023No Hit
TCTAGTCGGGGAGTTCTGCGTTATAAAGCCTGGAGAAAGCTAAGCTTAGAAC1750.10084246677961023No Hit
GTGCCTGGGAGACATGTATTTCTTGCACAGCTAATAGAAGACAAAGTTGAAA1750.10084246677961023No Hit
AAGCTTGGGAGAGAGAATTTAAAAAACAAGAGAGAAGCATGAAGTCACATAT1740.10026622411229817No Hit
AGCACGGGTGAATGGGTAAACCAGTTATATATTTTGCTTTCACAGCATGAGA1740.10026622411229817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)