FastQCFastQC Report
Sat 31 Dec 2022
SRR5849526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173953
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC7440.42770173552626284No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT7230.4156295091202796RNA PCR Primer, Index 23 (95% over 21bp)
TACTATGGGATTCTACATATTAATATCCCTGTGAAGTCATTACAACAGCTGT2320.1333693583899099No Hit
AAGACGGGAATCATATAATTAGTTTCAGAGTCAAATATTAAAATTATTTGGA2150.12359660368030445No Hit
TCATATGGGATTCAGACTCCTGGCTATGTATTGCACATTGGCAAGTGCTCTG2130.12244686783211559No Hit
TAAGCGGGGGATGTTCCTGGAAAAATTACATTTCAAGAATCTAAAACATTGA2120.12187199990802114No Hit
GAACTTGGGGGGCTCACCCTCGGGGGTCTGGCCGTGAGCGAGCACCGGCTCA2110.1212971319839267No Hit
GCTCTAGGGGGGTTCTATGGAATCTTTTCCATGTGCATGTATTTGTATCCTT2080.11957252821164337No Hit
CTATGCGGGACTGTCTCCAAGAAAACACAACCTGCCTTTTGGGGGTGGAGGG2080.11957252821164337No Hit
ATTAGCGGGCAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTAT2070.11899766028754892No Hit
AAGCATGGGCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACT2050.11784792443936004No Hit
GGTTATGGGTAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGC2050.11784792443936004No Hit
GAAACAGGGCTGATAACCTCTTTTGCCAACTCGCATATCTGTGTAGTTTTTC2040.11727305651526561No Hit
AATGCAGGTCTGCCAAACAATAATAAAGCCAACTGGAAACGAATCGGAGCCA2010.1155484527429823No Hit
TACACCAGGGATCCGTGATTTCATCCTTATGTGAAAAAGGGCTTAGTACTGT1990.11439871689479342No Hit
GCATGGGGAATGGAAGAAGTCTTCTGTTGTATAGTAACAATTGGTCAGGTTG1990.11439871689479342No Hit
CAGCATTGATACCCAATGACAAACTATGGAGAACAAGCAAAGTATGCAGGCC1990.11439871689479342No Hit
GCAACTGGGCAGGTGGTCCATCCACTTTGGCCTCCCAAAATGTTGGGATTAC1980.11382384897069897No Hit
AAGTAGGGGGATAAGAAAAGGAAGACAAATTTATGCTTATCTATAGTACAGA1940.11152437727432123No Hit
GTTGCGGGAACAAAGAAGTTGTGAGTATATTATCTACATTTTAGAAAATTTT1940.11152437727432123No Hit
GCACACGGGGGTATCTAACTTTTAGCAAAGGAGGGTTGCAGAGCAGCTTAAA1920.11037464142613235No Hit
ATATGTGGGGAAATCTGAAATCTTTGTAAGATTATTCAGCTCTTGTGCTGGT1900.10922490557794347No Hit
CGTCTAGGGGGCTTCTCCATAGGCAGAAAACTAGCGTAGTTTTTTTATTAGA1900.10922490557794347No Hit
GCATTAGGGGTTAAAAGTCAAACATATTTGGTATTGCCAAGTCGACTGAGAT1900.10922490557794347No Hit
GTTGTTGGGACCTCCCACCCACTTGCAGTCTACTTTCTGTTTCTATGGATTT1900.10922490557794347No Hit
GCATCCGGGATCTGAATGTAAAAGGAATCAACGGCTAAATCAAGCTTAAAGT1880.10807516972975459No Hit
TCTAGTCGGGGAGTTCTGCGTTATAAAGCCTGGAGAAAGCTAAGCTTAGAAC1870.10750030180566016No Hit
TAACTTGGGATTAATTTCAAGATAAAGTTTTAATTAGCTAAAACTTTTCATT1860.10692543388156571No Hit
CCAGCCTGGGCAACAGAGCGAGACTCTTGTCTCAAGAAGAAGAAAAAAAGAA1860.10692543388156571No Hit
AGCACGGGTGAATGGGTAAACCAGTTATATATTTTGCTTTCACAGCATGAGA1860.10692543388156571No Hit
TTGTACGGGCACAACCCAAATATTCATTAGCCGATGAATGAATAAACAAAAT1850.10635056595747128No Hit
ACGACAGGGCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAAT1850.10635056595747128No Hit
AATATTGGGGTAATTTGCCTCAATAAAAGTTTTAAATAATACAATTTTATCC1840.10577569803337683No Hit
TAGTGCAGGGTAAGAGTTTTATTGGAAGAAAAAACACTGTCAGAGCTTACTG1830.1052008301092824No Hit
TGCCTGGGGAGAACAGATCTTTTACTTTGGCCGCTTGAAAAGCTAGTGTACC1830.1052008301092824No Hit
TGCTATAGGGGATCTCGATCAGGGACTTGTCCCAAATCAGAAACAATTGGCG1820.10462596218518795No Hit
CAAAACGGGTACCTGTTAGATTAAATTACCTTGAGTTCAAATAATATCAGTT1810.10405109426109352No Hit
TTGCATCGGGACCATAGAGGATTCCAGAAGTGTGATCATTGTGCCCTTCTCT1810.10405109426109352No Hit
GCTTCTGGGATTGAATTACTGACTTGAGATCTTTTTTAATGTAGGTATTTAT1810.10405109426109352No Hit
ACAGCGGGGGCCCAGCACAATATTTCTTAGCCTACAGTGATAACCAATTTGA1810.10405109426109352No Hit
ATAATTGGGAAGGCATTGAATCTCTAAAATGCTGTTCTCAATCATTGTCAGA1790.10290135841290464No Hit
GCTACAGGGGGAGTCTCCCAGCTGCCAGCCGACCCCGAACCCGCACTCCCGC1790.10290135841290464No Hit
GCACTTGGGATCCATCCCAGAGGTAGTAACTAAGACCTGTTTTTAGACATTG1790.10290135841290464No Hit
AAGCAAGGGTCTACGAAAGGGATGACACTGACACTGCTATTTTGAAACCTGG1790.10290135841290464No Hit
AATACCGGGGCAAAAAACAAAATTTGATGTTGAGAGGCTAACATAATTAGTG1790.10290135841290464No Hit
CATCCTGGGATATTTAGAGTTCCATTTCAGATTTCTTCTTTGCCACCCTTTT1780.1023264904888102No Hit
GCTTTTGGGGTTTCCTAGAAATTAGTGGTTTTGTTTGGGAATTAGAAAAATT1780.1023264904888102No Hit
TTCCCAGGGCAGTCTCTTGTCTCAGGCTCTTCACCTTGTTACCAATCCTCGT1770.10175162256471576No Hit
GCATGAGGGGAGGTTTACCACAACCTGAAGAATGTCATCAAGGAGAAATATG1770.10175162256471576No Hit
GCCTATGGGCAATTGAAGCAGCCCGTGGTACCATCACAGTATGCAGAGACTT1770.10175162256471576No Hit
TTATGAGGGGGGTAGATAGTTTTGTAGAAGACTAGAAATCAGTTTCTAAATG1760.10117675464062133No Hit
TCTCTTGGGGTAAAACTGCTCATCTAGCCATGGAGTTTACCTTCACCTCCAA1760.10117675464062133No Hit
ACCTTGGGGAATGTAATGCAGTAATTAATTGCAAATACCACTCAAACTTAAA1760.10117675464062133No Hit
TCTACCGGGCAAGGCTTCTCCTCCAGGTCTGACAAGAAAGGGCCAAAGACCA1750.10060188671652688No Hit
GTATGAGGGGTATCAGGATTAAATAATCTGAATTTCTGAAAGCTAGCCTAAG1750.10060188671652688No Hit
CCACCAGCGGCAGGCTTACAGGCGTGAGCCACTGTGCCCGGCTCACAGGGTA1740.10002701879243245No Hit
AGAACAGGGCAAAGTGCTGGGATTACAGGCGTGAGCCACCATGCCTGGCCTA1740.10002701879243245No Hit
CTGGGGCCCGAGGGATTCAGATGCTAATGGGGACACAGGGGCCACTTGGTGT1740.10002701879243245No Hit
GTGCCTGGGAGACATGTATTTCTTGCACAGCTAATAGAAGACAAAGTTGAAA1740.10002701879243245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)