FastQCFastQC Report
Sat 31 Dec 2022
SRR5849525.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849525.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170067
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT7610.4474707027230444RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC6450.3792622907442361No Hit
GCTCTAGGGGGGTTCTATGGAATCTTTTCCATGTGCATGTATTTGTATCCTT2280.13406480975145088No Hit
TAGTGCAGGGTAAGAGTTTTATTGGAAGAAAAAACACTGTCAGAGCTTACTG2150.12642076358141205No Hit
GGTTATGGGTAAGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGC2120.12465675292678768No Hit
TACTATGGGATTCTACATATTAATATCCCTGTGAAGTCATTACAACAGCTGT2080.12230473872062188No Hit
ATTAGCGGGCAGTGCAGCTCCACATTAACTCTACAGACCAAACCATTTGTAT1960.11524869610212446No Hit
ACGCTAGGGGGACATGGGTACCAATTGCTTTTGTAATATGGTAAAAATGTGA1950.114660692550583No Hit
AGAACAGGGCAAAGTGCTGGGATTACAGGCGTGAGCCACCATGCCTGGCCTA1930.11348468544750011No Hit
AAGCAAGGGTCTACGAAAGGGATGACACTGACACTGCTATTTTGAAACCTGG1920.11289668189595865No Hit
GTGCCTGGGAGACATGTATTTCTTGCACAGCTAATAGAAGACAAAGTTGAAA1910.11230867834441721No Hit
GCAACTGGGCAGGTGGTCCATCCACTTTGGCCTCCCAAAATGTTGGGATTAC1900.11172067479287574No Hit
ATAATTGGGAAGGCATTGAATCTCTAAAATGCTGTTCTCAATCATTGTCAGA1880.11054466768979285No Hit
GTTGCGGGAACAAAGAAGTTGTGAGTATATTATCTACATTTTAGAAAATTTT1880.11054466768979285No Hit
GTATGAGGGGTATCAGGATTAAATAATCTGAATTTCTGAAAGCTAGCCTAAG1880.11054466768979285No Hit
CTGGGGCCCGAGGGATTCAGATGCTAATGGGGACACAGGGGCCACTTGGTGT1860.10936866058670995No Hit
AATAGCAGGGGATTAAAAAGCGAATGGGTCCCGGAGCAACAGCTGGTGGTGC1850.1087806570351685No Hit
ATATGTGGGGAAATCTGAAATCTTTGTAAGATTATTCAGCTCTTGTGCTGGT1840.10819265348362703No Hit
GCATGGGGAATGGAAGAAGTCTTCTGTTGTATAGTAACAATTGGTCAGGTTG1840.10819265348362703No Hit
TTTAACGGGGTGGCACAGACTTTGAAGCCCAATTGTAAAACCAGTTCAGTTT1830.10760464993208559No Hit
GTGTGTGGGTTTATAGAAACAAAATAGCTACTATAACTCTATTATAGTATAT1810.10642864282900269No Hit
GCACTTGGGATCCATCCCAGAGGTAGTAACTAAGACCTGTTTTTAGACATTG1810.10642864282900269No Hit
GAACTTGGGGGGCTCACCCTCGGGGGTCTGGCCGTGAGCGAGCACCGGCTCA1800.10584063927746123No Hit
CGATACGGGAGGATATTTTCATTTATGATGGGTTTATCAGGAAGTAACCCCA1800.10584063927746123No Hit
CGTTCAGGGGAGACAGTTTTCTTCATTAGGCCTGAGAGGCAAAGGTGTGATA1800.10584063927746123No Hit
TCATATGGGATTCAGACTCCTGGCTATGTATTGCACATTGGCAAGTGCTCTG1780.10466463217437834No Hit
TAACTTGGGATTAATTTCAAGATAAAGTTTTAATTAGCTAAAACTTTTCATT1780.10466463217437834No Hit
TTTGGCGGGGGGTTTGAGGCTTAAACAAGCCAACATATGAATATATGTTTTG1780.10466463217437834No Hit
AGCTTGGGGTCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC1770.10407662862283687No Hit
AATGCAGGTCTGCCAAACAATAATAAAGCCAACTGGAAACGAATCGGAGCCA1760.10348862507129544No Hit
AATACCGGGGCAAAAAACAAAATTTGATGTTGAGAGGCTAACATAATTAGTG1760.10348862507129544No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1750.10290062151975397No Hit
TGATGCGGGAGGTGTGCTGTGGTGCACTGCAGTGATGTATTTGCATAACATT1750.10290062151975397No Hit
GCTATGGGGACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCAT1740.10231261796821253No Hit
CTTAAGGGTGAATAAATGTATGAGTCAATGAATGAGGTTGCAGAGTTATCAA1730.10172461441667108No Hit
AAGTAGGGGGATAAGAAAAGGAAGACAAATTTATGCTTATCTATAGTACAGA1720.10113661086512962No Hit
GCATTAGGGGCCACCTCCATTTATTTCCCCACTGCTCCTCCACTGACATCCT1710.10054860731358818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)